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Methods]The total proteins of untreated and ethambutol treated mc2155 cells were separated by ampholyte pH gradients based two-dimensional gel electrophoresis (2-DE). The differential expression proteins were analyzed by using image analysis software.

方法]采用载体两性电解质pH梯度双向凝胶电泳技术分离乙胺丁醇处理前后耻垢分枝杆菌mc2155细胞的总蛋白质,用PDQuest 7.3.1图像分析软件比较分析,以识别差异表达的蛋白质。

Objective:To study the change and clinical significance of antithrombin Ⅲ and its clinical value in nephropathy syndrome patients before and after treatment.

目的 :研究肾病综合征患者在治疗前后抗凝血酶Ⅲ的变化及临床价值。方法:分正常对照组、肾病综合征治疗前及治疗后三组,应用全自动血凝仪测定AT -Ⅲ活性。

PRA serum samples of children patients with thalassemia after repetitive blood transfusion, five samples of AB blood grouping serum, and six samples of positive anticoagulation vein blood (10 mL) were used in the study.

反复输血地中海贫血患儿的群体反应性抗体血清样本。5份健康儿童AB分型血清样本。6份阳性抗凝静脉血样本10 mL。

Ta group: After retinal vein was occluded by the same method used as above, a venipuncture is performed in the locations away from the occluded site about 2/3 to 1 PD distance, a 20G needle tip was used to puncture the retinal vein and underlying retina and the Bruch s membrane.

2手术方法:局部麻醉,标准三切口扁平部玻璃体切除术,术中作玻璃体后界膜的剥离和切除。在四象限视网膜分枝静脉距视盘2一3PD选定的吻合部位,预做局部视网膜光凝,切开静脉管壁和其下的视网膜和Bruch's膜,气/液交换,12%CZF。

Based on hemagglutination specificity test,absorption and eluate test,and hemagglutination inhibition assay,it was showed to be specific only for the human H blood ...

杂交瘤细胞在液氮中贮存1年,分泌抗体能力不变。抗体经56℃保温30min及-20℃和37℃反复冻融试验,凝血活性保持稳定。可望能取代传统的抗H试剂而用于临床血型分型。

The reactor is shared in two parts, with different flocculation energy.

反应池分两部分,每部分的絮凝能量有所差别。

The effect of solids loading,dispersant content and pH on rheological properties of slurry and properties of green body with gelatine as coagulant are studied in this paper.

研究了以氧化铝为原料,以明胶为胶凝剂的注模成型工艺中固含量、分散剂、pH值等对浆料流变特性及素坯性能的影响。

Prepare 131I-labbelled anti-CD20 monoclonal antibody using lodogen iodine labelling;(2)Measure the immunity activation of labbelled antibody by means of both cell combine analysis and LDH cytotoxicity detection kit;(3)Evaluate the injury rate of tumor exposed to 131I-chimeric anti-CD20 monoclonal antibody in vitro. The MTT method and the experiment of cell growth control are used;(4)Record the apotosis of Daudi cell using gelose electrophoresis;(5)Learn the inhibition effect of radioactive medication on the marrow. Radiohnmunoimages are taken on various intervals after injection of the labeled antibodies to the nude mice. The distribution of radioactive medication, I31l-labeled antibodies, in the marrow tissue indicates the inhibition effect. Here a B cell non-hodgkin lymphoma model in nude mice is established by us;(6)28-day observation are done in 3 groups of nude mice model.

(1) 用lodogen标记法制备131I-国产人鼠嵌合抗CD20单抗;(2)采用细胞结合分析法和乳酸脱氢酶法细胞杀伤性实验测定131I标记后国产人鼠嵌合抗CD20单抗与靶细胞的免疫活和利结合能力;(3) MTT法和细胞生长抑制实验测定131I-国产人鼠嵌合抗CD20单抗的体外杀瘤活性;(4)用琼脂糖凝胶电泳法研究131I-国产人鼠嵌合抗CD20单抗致Daudi细胞凋亡;(5)建立荷人B细胞淋巴瘤移植瘤裸鼠模型,应用γ计数法检测注射到荷瘤裸鼠体内的131I-国产人鼠嵌合抗CD20单抗的组织分布情况,明确其靶向性;(6)将荷瘤裸鼠分3组进行放射免疫治疗,分别为阴性对照组、131I-国产人鼠嵌合抗CD20单抗组、国产人鼠嵌合抗CD20单抗组。

Methods: 127 cases of patients with steroid-induced osteonecrosis after Syndromes, and lipid testing, hemagglutination testing and test results contrast hemorheology.

对127例激素性骨坏死患者辨证分型后,与血脂检测、血凝检测以及血液流变学检测结果相对照。

Methods XbaI was used to digest the total DNA of Salmonella enteritidis strains isolated from the food poisoning samples.

对食物中毒中分离的肠炎沙门菌用XbaI酶切总DNA,用脉冲场凝胶电泳法进行分子分型,用BioNumerics软件对PFGE分型图谱进行电子化数据分析,绘出聚类分析图。

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Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克,果实色泽鲜艳,汁多味浓,甜酸适口,离核仁甜,清末时就驰名陕、甘两省,清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异:红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感,ED90值分别为47.65、71.67和29.17g/hm2;春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感,ED90值分别为96.91、114.20g/hm2;而番茄不敏感。

However, I have an idea.

不过,我有个主意。