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Two pairs of primers were designed based on the sequence of ovis aries preprochymosin cDNA reported in GenBank.The preprochymosin cDNA was obtained from toltal RNA isolated from the abomasum of 5-day-old Xinong Saanen Goat by RT-PCR method and then cloned successfully in E.coli.

参照GenBank登录的绵羊凝乳酶原前体cDNA序列设计引物,以新生5d的西农萨能羊皱胃组织总RNA为模版,通过RT-PCR方法获得凝乳酶原前体cDNA,克隆测序后进行序列比对。

Examples include trypsinogen and chymotrypsinogen, secrete d by the pancreas and converted by proteolysis in the small intestine into the active enzymes trypsin and chymotrypsin; and numerous coagulation factors.

这作用受不同的催化或受的活化型本身的催化。原细胞合成并贮存钝化型式的原。胰脏分泌的胰蛋白原及胰凝乳蛋白原在小肠内活化成胰蛋白及胰乳凝蛋白。

Examples include trypsinogen and chymotrypsinogen, secreted by the pancreas and converted by proteolysis in the small intestine into the active enzymes trypsin and chymotrypsin; and numerous coagulation factors.

原细胞合成并贮存钝化型式的原。胰脏分泌的胰蛋白原及胰凝乳蛋白原在小肠内活化成胰蛋白及胰乳凝蛋白。到中国译典经典版本中查找关于proteolysis的最新解释和例句。。。。

Lact acid bacteriaexopolysaccharides has some characteristics of improving yoghurt qualities,such asenhancing yoghurt viscosity and rheology,prohibiting whey loss,improving yoghurttaste and increasing clabber intensity.

乳酸菌胞外多糖具有明显改善酸乳的品质的特性,如增加酸乳的粘度、流变性、防止乳清析出、增强凝乳强度、改善酸乳口感等。

We compared the number of integral membrane proteins identified from the PM of human CNE1 cells by PAOT with that of an optimized trypsin/chymotrypsin method. More than double the total number of identified IMPs was obtained with PAOT, mainly contributed by the IMPs with positive grand average of hydropathicity scores.Part 2: We describe a procedure for digesting the hydrophilic domains in the absence of detergents and denaturants.

同时,我们以人类CNE1细胞系为材料,比较了PAOT与另外一种优化好的胰蛋白酶/胰凝乳蛋白酶酶解方法,发现PAOT鉴定到的整合膜蛋白是胰蛋白酶/胰凝乳蛋白酶酶解的2倍,并且这些整合膜蛋白的疏水值大部分都为正值。

The development of genetically engineered chymosin has been encouraged by shortages and fluctuations in cost of rennet from calves.

发展基因工程凝乳酶深受鼓舞短缺和价格波动的凝乳酶的牛犊。

For its production, milk is coagulated at 32-34 oC. The obtained curd is divided after 35 minutes.

制作奶酪时,奶在32-34℃下凝乳,约35分钟后凝乳完成,凝乳块随后被缓慢加热到48℃。

There is vegetable rennet available and many organic dairies produce cheese with it instead; however in aged cheese, the animal rennet also breaks down into other enzymatic patterns over time that it does not retain the slaughter thought-form associated with its origins.

有可用的植物性凝乳酶,许多有机乳制品店做乳酪时用它来代替动物性凝乳酶;但是在陈乳酪中,动物性凝乳酶随时间也分解为其它生化酶模式,不再保留与它来源相关的屠杀之思想形态。

In order to investigate the mechanism of coagulant milk, the paper studied temperature's impact on coagulant milk, prepare ginger protease crude extract, oleoresin and essential oil, and do experiments espectively.

为了探讨姜汁凝乳的机理,研究了温度对姜汁凝乳的影响,制备了生姜蛋白酶粗提物、姜油树脂和姜精油,并分别进行了凝乳实验。

ResultsRecombinant bovine prochymosin was not significant difference in molecular weight,immune characteristics, bioactivity and sensitivity of inhibitors comparison to nature bovine prochymosin.The milk-clotting activity of recombinant bovine prochymosin was 2×103 international milk-clotting unit per millilitre.

重组牛凝乳酶原与天然牛凝乳酶原比较,其分子量大小、免疫性质、生物活性和抑制剂敏感性没有发现显著差异,其凝乳活性可达2×103 IMCU/mL。

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