再聚合

Methods The total RNA was extracted from the brain of human embryo. The segment of open read frame of DOC-1 was amplified by RT-PCR and was further cloned into the vector, pMD18-T, and the prokaryotic expression vector, pGEX-4T-1, by turns, to produce the new construct pGEX-4T-1-DOC-1. After restriction enzymes digestion analysis and sequenced, pGEX-4T-1-DOC-1 was transformed into E.coli BL21（DE3）. GST-p12 recombinant protein was expressed under IPTG induction and purified by affinity column.

从人胎脑组织中提取总RNA，经逆转录-聚合酶链式反应扩增DOC-1的CDS序列，再通过基因重组技术将该基因片段依次克隆到pMD18-T和pGEX-4T-1载体中，构建融合表达载体pGEX-4T-1-DOC-1，经酶切、测序鉴定后，用该重组质粒转化E.coliBL21，用IPTG诱导表达，Glutathione Sepharose 4B柱亲和层析纯化重组蛋白。

For the first time ,AgBr organosol was obtained by using oleic acid to extract and modify the AgBr nanoparticles in hydrosol. And the optimum molar ratio of oleic acid to AgBr nanoparticles was found. AgBr nanoparticles, modified by oleic acid were mixed with MMA. After polymerization was carried out, the polymeric photochromic material was synthesized.

首次采用油酸萃取并包覆了水溶胶中的AgBr纳米粒子，制备了AgBr有机溶胶；并找到了萃取率最佳时的油酸与AgBr的摩尔比；再将油酸包覆的AgBr掺杂到甲基丙烯酸甲酯中，进行本体聚合，从而制备了AgBr光致变色有机玻璃。

By using ethanol precipitative pretreatment and rectification patent technology,the product 1,3-propanediol with purity of 99% had been harvested,the seperation yield of 1,3-propa...

采用发酵液醇沉预处理、再精馏的专利技术可分离得到纯度大于99%的1，3-丙二醇产品，分离收率大于85%，产品质量达到了聚对苯二甲酸丙二酯聚合反应的要求。

Currently, the Company has developed a 10 sodium borate to the main raw materials of "instant Boron efficient products - polymerization B", import boutique borax-based raw materials,"Shi-based products for Boron - strong boron and boron lot" and "Good buy" series of fertilizer, product selection of raw materials, scientific formula to meet the nutritional needs of crops.

目前，本公司已开发出以十硼酸钠为主原料的"高效速溶硼肥产品——聚合硼"、进口精品硼砂为主原料的"基施专用硼肥产品——强力硼、硼多多"及"好再买"系列微肥，产品精选原料，科学配方，能够满足作物的营养需求。

For most viruses,there is aneed for antimicrobials that target unique viral molecular properties.Acycloviris one such drug.It is activated into ahuman herpesvirusDNA polymerase inhibitor exclusively by HHV kinases and,thus,does not suppress other viruses.Here,we show that ACV suppresses HIV-1in HHV-coinfected human tissues,but not in HHV-free tissue or cell cultures.However,addition of HHV-6-infected cells renders these cultures sensitive to anti-HIV ACV activity.We hypothesized that such HIV suppression requires ACV phosphorylation by HHV kinases.Indeed,an ACV monophosphorylated prodrug bypasses the HHV requirement for HIV suppression.Furthermore,phosphorylated ACV directly inhibits HIV-1reverse transcriptase,terminating DNA chain elongation,and can trap RT at the termination site.These data suggest that ACV anti-HIV-1activity may contribute to the response of HIV/HHV-coinfected patients to ACV treatment and could guide strategies for the development of new HIV-1RT inhibitors.

对大多数病毒而言，都需要有针对其分子特性的靶向杀毒剂阿昔洛韦就是这样一种靶向药物在人疱疹病毒酶的特定作用下，阿昔洛韦被激活成为人疱疹病毒DNA聚合酶抑制剂，因此不能再抑制其它的病毒我们的研究发现阿昔洛韦在共感染人疱疹病毒的组织中可以抑制HIV-1，但在无人疱疹病毒感染的组织或细胞中无此作用然而，加入人疱疹病毒-6感染的细胞却使得其对抗HIV的阿昔洛韦变得敏感我们推测这种抑制作用依赖于人疱疹病毒酶导致的阿昔洛韦磷酸化实际上，单磷酸化的阿昔洛韦前体药物无需人疱疹病毒的参与即可抑制HIV此外，磷酸化的阿昔洛韦能直接抑制HIV-1逆转录酶，将其阻止在终止位点，从而终止DNA链的延长这些结果提示阿昔洛韦的抗HIV-1活性决定了艾滋病病毒/人疱疹病毒共感染的患者对阿昔洛韦的治疗反应，也有助于开发新的HIV-1逆转录酶抑制剂

The acetated and hydrogenated urushiol dimer has been separated,and studied by hetero-J resolved 2D-NMR,homo- and heteronuclearchemical shift correlation 2D-NMR and long-range coupled chemicalshift correlation 2D-NMR.The results show that the urushiol dimerare most diphenyl and alkyl phenyl ether structures.Furthermore,IR and MS data also has conformed this structures.

本文将酶催化预聚后的生漆，催化加氢后，乙酰酯化，再进行离心旋转薄层层析，分离得到二聚体主要组分，用异核J分辨谱，同核化学位移相关，异核化学位移相关，远程偶合异核化学位移相关等2D—NMR技术，全面研究了其结构，得到了联苯型和烷基芳醚型聚合结构的结论。

In this thesis, monomer with unsaturated double bond was firstly synthesized byreacting acryloyl chloride with bifunctional monomer. Such synthesized monomer ormonomer with initial unsaturated double bond was used to prepare linear polymericmaterials by free radical polymerization. A series of new degradable space polymerswith acylamino bond were subsequently synthesized using diisocyanate as thecross-linker.

本文首先通过丙烯酰氯和双官能团单体反应引入不饱和双键，或者直接使用具有不饱和双键的单体，通过自由基聚合得到含有活泼羟基或氨基的线型高分子聚合物，再用二异氰酸酯作为交联剂交联，从而合成了一系列基于酰胺键的新型可降解高分子体型聚合物。

The sodium sulphonate of ethoxylated acrylic modified rosin can be obtained from acrylic modified rosin after the reaction with ethylene oxide and then be sulphonated with chlorosulphonic acid.

以松香为原料，用丙烯酸进行改性后再与环氧乙烷聚合，然后用氯磺酸进行磺化处理制得丙烯酸改性松香聚氧乙烯醚磺酸钠阴离子表面活性剂。

Dimethylamino)ethyl methacrylate was polymerized using PS-CH_2Cl as macroinitiator and CuCl/ 2,2 '-Bipyridyl as catalyst in toluene to acquire amphiphilic brush-type copolymers of PS-g-PDMAEMA.

再以氯甲基化的聚苯乙烯(PS-CH_2Cl)为大分子引发剂，在CuCl和2，2′-联吡啶(2，2′-Bipyridyl，bpy)存在下，以甲苯为溶剂，进行甲基丙烯酸N，N-二甲胺基乙酯2-(Dimethylaminoethyl methacrylate，DMAEMA的ATRP聚合，得到两亲性的梳状共聚物PS-g-PDMAEMA。

Clustering results show that jihebai A combined with jihebai B first,and then combined with the Large White,with the Liaoning Black and Tanyu Black together; combined with Duroc and Landrace in turn.

聚类结果表明，冀合白A与冀合白B聚合在一起，然后与大白猪聚在一起，再与辽宁黑猪和丹育黑猪聚在一起；杜洛克、长白猪与他们依次聚在一起。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。