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Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

中文题名斜带石斑鱼生长激素分泌及其基因表达调控的研究副题名外文题名 Regulation of growth hormone and its gene expression in orange-spotted grouper,Epinephelus coioides 论文作者冉雪琴导师林浩然学科专业水生生物学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数140页关键词生长激素基因表达石斑鱼馆藏号BSLW /2003 /Q959 /39 应用分子克隆技术,构建了斜带石斑鱼生长激素(Growth hormone,GH)基因重组质粒,在大肠杆菌细胞中获得高效表达,亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原,免疫家兔制备特异性抗体,通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交/核糖核酸酶保护试验及化学发光检测技术,研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制,对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义

The seed production of Quercus wutaishanica is important for the regeneration and restoration of its population.

辽东栎种子产量对其种群更新和恢复具有重要意义

Audiological and radiological examination play an important role in early diagnosis and treatment.

听力学和影像学检查对早期诊断、及时治疗具有重要意义

It is of great significance of studying the genetic reassortment, evolution and its biological characteristics of this virus strain to prevent and control the pandemic.

研究该毒株的基因重配、进化历程及其生物学特性,将对防控此次流行具有重要意义

The change has an impact on a part of inhabitants who inhabited in employee dorm housing formerly, as a result, they rechoose residences which results in inhabitant reorganization and trip distribution in the city.

合理有效地预测城市居民出行分布规律对城市土地利用规划、城市交通规划、城市总体布局规划等都具有重要意义和参考价值。目前国内外在预测居民出行分布时普遍采用的模型主要有增长系数模型、重力模型、介入机会模型等。

Gastrointestinal medical informations which can refect the pathologic changes of GI dysmolities are crucial for early diagnose and prophylactic treatment of digestive system diseases.

胃肠道医学信息可反映胃肠道动力障碍性疾病生理病理改变情况,对消化系统疾病的早期诊疗及防止消化器官的恶性病变具有重要意义

Determined the formation mechanism and their time—space distributgion pattern, the following conclusion were drawn: the key element in establish a depositional system is the identification of exceptional reflectional stracture, and the identification of the palaeodrainagte pattern have extrordinary influence on the restoration of the depositional environment.

建立了侏罗—白垩系青山口组共5个时期的沉积体系分布图,指明了沉积体形成机制和时空展布特征,并认为:识别特殊反射结构是建立沉积体系的关键,判断古水系对恢复沉积环境具有重要意义

Studying the influence of milk protein pel-licles on demineralization and remineralization of tooth surface can further ensure the mechanisms of protection against dental caries of milk .

研究牛奶蛋白膜对牙表面矿化的影响,对于进一步理解牛奶的防龋作用具有重要意义

This thesis would contribute to rescan the status of our country's higher education and make effective countermeasure to promote the higher education international competitiveness.

对中国和印度的高等教育竞争力进行研究,从比较的角度发现问题,重新审视我国高等教育竞争力在国际上的地位,对科学制定提升高等教育国际竞争力的对策具有重要意义

The Jiangxi Province countryside area liver cancer prevention should put with emphasis is fostering the good life health custom, the maintenance open and bright and the optimistic disposition, advocates no smoking, stopping drinking, strengthens the exercise, the reasonable meals, enhance the organism resistively, to prevents the liver cancer the occurrence development also to have the vital significance.

江西省农村地区PHC的预防重点应放在养成良好的生活卫生习惯,保持开朗和乐观的性格,提倡戒烟,戒酒,加强锻炼,合理膳食,提高机体抵抗力,对预防PHC的发生发展也具有重要意义

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Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克,果实色泽鲜艳,汁多味浓,甜酸适口,离核仁甜,清末时就驰名陕、甘两省,清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异:红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感,ED90值分别为47.65、71.67和29.17g/hm2;春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感,ED90值分别为96.91、114.20g/hm2;而番茄不敏感。

However, I have an idea.

不过,我有个主意。