假细菌

Results The results showed that the average concentration of the bacteria, fungi and total microbes in the outdoor environment were 5.04×10^4, 3.75×10^3 and 5.41×10^4 cfu/m^3 respectively and the fungi accounted for 6.92% of the total. The preliminary identification showed that the dominant bacteria mainly were Micrococcus, Bacillus, Pseudomonas and Staphylococcus and the average percentage were 37.3%, 21.0%, 10.3% and 9.5% respectively. Of the five fungi the dominant ones were Aspergillum, Penicillium, Mucor and Rhizopu. The average percentage were 42.0%, 36.0%, 4.4% and 4.2% respectively. Conclusion The outdoor air of Hefei has been polluted by airborne microbes.

结果 合肥城区空气细菌总数均值为5.04×10^4cfu/立方公尺，真菌总数均值为3.75×10^3cfu/立方公尺，合计为5.41×10^4 cfu/立方公尺，其中真菌占6.92%；对不同采样点的空气细菌和真菌进行了初步鉴定，优势细菌属为微球菌属、芽孢杆菌属、假单胞菌属和葡萄球菌属，分别占总细菌的37.3%，21.0%，10.3%，9.5%；真菌共检出5属，其中优势菌属为曲霉属、青霉属、毛霉属、根霉属，分别占总真菌的42.0%，36.0 %，4.4%，4.2%。

In the condition of the above mentioned, field experiments were done. The results showed that:The process was the inoculation proportion of Candida tropicalis, Pseudo monas and Candida utilis as 1:2:2, inoculation 2%, 2.0% urea added in Jowar juice, pH 6.0, fermenting 72h at 30℃. The content of fulvic acid could reach more than 20%.5% mixture of Azotobacter chooroccum, Phospho bacteria and Silicate bacteria (2:1:2) were added to the product. The total number of bacteria reached 10 /ml.

研究结果表明：用热带假丝酵母、假单胞杆菌和产朊假丝酵母组合进行液体发酵，接种比例1∶2∶2，接种量2%，在甜高粱秸秆汁中加入2.0%的尿素，pH值为6.0，发酵温度为30℃，发酵72h，发酵产物中生化黄腐酸含量可达20%以上；将圆褐固氮菌、磷细菌、钾细菌以2∶1∶2的比例，按5%加入到发酵产物中，三种细菌总的活菌数可达10~8个/ml；生化黄腐酸复合生物液肥对各种作物的产量均有不同程度的提高，经济效益显著。

Go in all bacteriological examine 94 second, achieve positive result 79 second, rate of positive sending check is amounted to 84.04%, unplug before the canal every week 1 in all 64 bacteriological in examining, electronegative 8 second, occupy 12.5%, pure bacterium affects 52 second, 2 serious infection 1 second, bacterium, fungus mixes infection 3 second, the pathogenic bacteria inside spirit way is phyletic with change bacili of negative of orchid family name is given priority to, bacterium of verdigris holiday single born of the same parents is the first place.

结果首次细菌学检验有23例分离出致病菌，阳性率65.7%，其中革兰氏阴性杆菌17例，占73.9%。35例共行细菌学检验94例次，获得阳性结果79例次，送检阳性率达84.04%，拔管前每周1次共64次细菌学检验中，阴性8例次，占12.5%，单纯细菌感染52例次，二重感染1例次，细菌、真菌混合感染3例次，气道内致病菌种类以革兰氏阴性杆菌为主，铜绿假单胞菌为首位。

Consider to make clear in recent years, second acid is calcic - Bowman does not move bacillus clinical bacterium of single born of the same parents of the detached rate that go up and verdigris holiday comparatives, have the tendency that year after year rises, the fundamental condition pathogenic bacteria that already became the infection inside the courtyard [1] , have to a variety of antibacterial content be able to bear or endure higher property of a medicine [1, 2] , for this, we are monitored and statistic analysed my courtyard to came 1998 2004 7 years a second acid is calcic - the clinical infection condition that Bowman does not change bacillus, to 14 kinds of antibacterial content be able to bear or endure property of a medicine and be able to bear or endure medical tendency, sum up a report to be as follows now. 1 material and method all data all come from 1.1 basic materials to came on January 1, 1998 my courtyard outpatient service was mixed on December 31, 2004 the specimen sending check of inpatient, via clinical microbiology the lab is fostered and classics appraisal is second acerbity calcium - Bowman does not move bacillus to affect case of illness, with clinical and commonly used antibacterial at the same time content makes the kill that medical quick detects. 1.2 bacteria appraisal is mixed medical quick experiment uses live thing VITEK32 of Mei Li dust is full automatic the bacterial appraisal of bacterial analysis system and its form a complete set gets stuck and medical quick card undertakes detecting, 1998~2000 year use GNI appraisal gets stuck, 2001~2004 uses GNI+ card.

近年探究表明，乙酸钙-鲍曼不动杆菌临床上的分离率和铜绿假单胞菌相当，并有逐年上升的趋向，已成为院内感染的重要条件致病菌[1]，对多种抗菌药物有较高耐药性[1，2]，为此，我们监测并统计分析了我院1998年至2004年七年间乙酸钙-鲍曼不动杆菌的临床感染情况，对14种抗菌药物的耐药性及耐药趋向，现总结报道如下。1材料和方法1.1基本资料所有资料均来自于1998年1月1日至2004年12月31日我院门诊和住院病人的送检标本，经临床微生物学实验室培养并经鉴定为乙酸钙-鲍曼不动杆菌感染病例，同时以临床常用抗菌药物作药敏检测的结果。1.2细菌鉴定和药敏试验采用生物梅里埃VITEK32全自动细菌分析系统及其配套的细菌鉴定卡和药敏卡进行检测，1998~2000年使用GNI鉴定卡，2001~2004使用GNI+卡。

Results The total intestinal bacteria of Grass carp of the control group was 6.6×10^8 CFU/g ,and the dominant flora included Aeromonas, Pseudomonas and Enterobacter; After 10 d feeding on the concentration of 2.5%, 5.0% and 7.5% Chinese herb medicine compound, the total intestinal bacteria reduced to 3.0×l0^7 CFU/g, 5.0×10^6 CFU/g and 1.5×10^6 CFU/g respectively, and the dominant flora changed to F. indologenes P. alcaligenes and X. campestris.

结果 对照组鱼种肠道内的细菌总数为6.6×10^8CFU/g，其优势菌群为气单胞菌、假单胞菌和肠杆菌；投喂2.5%、5.0%和7.5%中草药复方药饵10d后，其肠道内细菌总数均有所减少，分别为3.0×10^7CFU/g、5.0×10^6CFU/g和1.5×10^7CFU/g，其优势菌群为吲哚黄杆菌、产碱假单胞菌和野油菜黄单胞菌。

The effects of removal of organic nitrogen by different ammonibacterium were compared and the scale to the quantity of organic nitrogen which consumed by ammonibacterium is the quantity of ammoniacal nitrogen which is produced.

结果表明，芽孢杆菌属、假单胞菌属为人工湿地中氨化细菌的优势菌属；氨化细菌-1、氨化细菌-2及氨化细菌-5对有机氮的去除效果相对较好，分别达到46.2%、49.4%和52.6%。

Results totally 287 strains were isolated from the 256 positive samples , and the gram-negative bacilli were 225(78.4%,), the gram-positive coccus were 41(14.3%), snd the monilia were 21(7.3%).the distributions of clinical bacteria were respiratory tract(63.4%),urinaryract(7.0%),secretion(includingwound .3%),blood(5.9%),stool(5.2%), pucture fluid(4.9%), and other sites(7.3%). of all isolating bacterium,from the first to the fifth were ps.aeruginosa(19.5%),k.pneumoniae(16.7%), e.coli(14.3%), a.baumannii(11.8%) and psemal (10.1%).resistant rates of methecillin-resistant s.aureus,methecillin-resistant coagulase-negative staphylococci and vancomycin-resistantwere 88.2%、70.0% and 11.1% respectively;the incidence of e.coli and k.penumoniae produce extended speutrum beta-lactamase were 68.6%和65.2%, 44.6% of ps.aeruginosa isolates were resistant to imipenem; the highest examining rate of 21 kinds of monilia was candida albicans (66.7％),resistant rate of candida albicans to fluconazole and amphotricin b was 51.3％ and 1.3%.

结果 在254份检出细菌阳性标本中共培养出287株细菌，其中革兰阴性杆菌225株（78.4％），革兰阳性球菌41株（14.3%），念珠菌21株（7.3%），检出菌来自呼吸道标本占63.4%，其他标本各占5%左右；细菌检出占构成比前三位的依次为铜绿假单胞菌19.5%、肺炎克雷伯菌16.7%、大肠埃希菌14.3%；耐甲氧西林金黄色葡萄球菌、耐甲氧西林凝固酶阴性葡萄球菌和耐万古霉素的肠球菌的发生率分别为88.2%、70.0%和11.1%；大肠埃希菌和肺炎克雷伯菌的超广谱β-内酰胺酶的检出率分别为68.6%和65.2%，铜绿假单胞菌对亚胺培南的耐药率为40.2%；白色念珠菌对氟康唑的耐药率为81.3％，对两性霉素的耐药率为3.2%。

Six bacteria and five mildews have been separated from 119 strains, wliich grew well on the culture media contained lignin. The result of taxonomic identification is that XI, X2 and X3 belong to Pseudomonas, X4 and X5 belong to Xanihomonas, X6 belongs to Mycoplana. A preliminary study has been carried out on the capacity of lignin degradation of the 6strains of bacteria, and strains X2 and X4 were chose to carry out the experiments. The most favorable temperature for the 2 strains is 30癈, and the pH is 7; After being treated by ultraviolet rays, activity of all the three kinds of lignin-degrading enzyme produced by the strains can be increased greatly, the bacteria increasing rate is remarkably improved.

从119个菌株中分离筛选出了6株细菌和5株霉菌，它们可以在含有木质素的培养基上良好生长：细菌分类学鉴定结果为X1、X2和X3：假单胞菌属，X4和X5：黄单胞杆菌属，X6：枝动杆菌属；对6株细菌降解木质素性能进行了初步的研究，选定X2和X4为实验用菌种，两株菌适宜的生长温度为30℃，最适初始pH值为7；经紫外线照射诱变法处理，可使它们产生的三种木素分解酶活性均有明显的提高，细菌增长速率比诱变前明显提高。

The number of autoterophic bacteria were significantly lower than the heterotrophic bacteria. Majority of 21 strains of heterotrophic bacteria that isolated from water body were Pseudomonas. Majority of 25 strains of heterotrophic bacteria isolated from sediment were bacillus, microccus and Pseudomonas.Three genus of autotrophic bacteria were sulphuret bacteria, nitrify bacteria and nitrosobacteria that were capable of oxidizing H2S, S, FeS, oxidizing NH3 to NO2 and NO2 to NO3 respectively.

试验稚鳖池水中共分离出21株异养细菌，其中优势菌为假单胞杆菌属细菌；底泥中共分离出25株异养细菌，其中优势菌为芽胞杆菌属细菌，微球菌属细菌假单胞菌属细菌，三种自养细菌分别为具有氧化硫化氢、元素硫或硫化亚铁的硫细菌；具有氧化氨为亚硝酸的亚硝化细菌和能将亚硝酸进一步转化成硝酸的硝化细菌。

By suspending cell culture from the callus of P. thugbergii and by fluorescent observation, a new method, which is easy and sensitive to observe cell activity and to test the phytotoxicity of various bacteria, was used. This method can be utilized to study the bacterial toxins. Cultured filtrates of some strains of the nematode-carrying bacteria were bioassayed for their toxicity to suspending cultured cells from P. thugbergii callus. Strong toxic effects were observed by the filtrates of Pseudomonas spp., P. fluorescence, and Sphingomonas spp., these bacteria species are coincide with those in the early stage of pine wilt disease.

本试验使用了黑松愈伤组织的细胞悬浮培养方法，用荧光方法观察各种细菌对植物细胞的毒性测定，这种方法具对细胞的活性观察容易、灵敏度高等特点，证明可用于细菌的毒素研究；对松材线虫携带的细菌的部分菌株，用其培养滤液对黑松愈伤组织的悬浮培养细胞的生物测定初步证明，松材线虫携带的细菌中以假单胞菌、荧光假单胞菌和少动鞘氨醇单胞菌的滤液对黑松细胞具有较强的毒性，这与松树发病早期出现的细菌种类相一致。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。