倒置法

Result: The nm-HAP-sol present the dispersed and uniformity needle. A431cells inhibition ratio was maximizing at the 5 day in the 400mg/L nm-HAP-sol group, the 200mg/L nm-HAP-sol group and the 100mg/L nm-HAP-sol group, inhibition ratio of the 400mg/L nm-HAP-sol group, the positive control group and the 50mg/L nm-HAP-sol group was respectively (77.89±6.29)%,(77.46±8.26)% and (1.23±0.15)%. It was detected that the number of A431 cells were persistently increasing in the 50mg/L nm-HAP-sol groups while decreasing in the 400mg/L nm-HAP-sol group, the positive control group and abidingly increasing for 4 and 3 days then decreasing in the 100mg/L and 200mg/L nm-HAP-sol group by inverted microscope.

结果：超声乳化法配制的HAP纳米溶胶呈均匀分散的针状；400mg/L、200mg/L和100mg/L溶胶组对A431细胞抑制率在第5天达到各组最大值，此时400mg/L溶胶组与阳性对照组的抑制率分别为（77.89±6.29）%和（77.46±8.26）%， 50mg/L溶胶组抑制率为（1.23±0.15）%；倒置显微镜下见50mg/L溶胶组细胞数目持续增加，聚集成簇，细胞呈现多角形或梭形，胞浆透亮，而100mg/L 和200mg/L溶胶组的细胞数目分别增加至第4天和第3天开始下降，400mg/L溶胶组和阳性对照组细胞数目持续减少，细胞体积缩小，漂浮细胞增多。

The cells were round before adherence,and anomalism fusiform,triangle and polygon when adhered,monocaryon,1-3 nucleole by invert phase contrast microscopy observation.Alkaline phosphatase staining and mineralized nodules staining were positive(≥98.06%).The osteoblastic activity was the highest on day 10,which was observed by MTT and morphological method.

倒置相差显微镜形态观察结果显示，未贴壁的细胞呈圆形，贴壁生长的细胞呈不规则梭形、三角形或多角形，单核，有1~3个核仁；碱性磷酸酶和钙化结节染色均呈阳性（阳性率≥98.06%）；MTT法结合形态学观察结果显示，成骨细胞培养至第10d时活性最强。

This paper presents a new measure to eliminate the ground current for fault location of power cable and analyses the basic principle of inversion bridge method.

在倒置电桥法测量电力电缆故障的基础上，提出了一种消除接地电流的新方法。

Methods Through digesting human skin with collagenase type Ⅱ, cells of human eccrine sweat gland were isolated.

通过酶消化法分离人皮肤汗腺，微量加样器在倒置显微镜屏视下吸取游离的汗腺组织移入Hank平衡盐溶液，经纯化后予以原代培养。

The trial practice in China points out that the subject which can start the procedure and deny the umpirage of the rule can only be national judicial organization, and also discusses the following aspects: the rule of honesty and credit, the rule of forbidding abusing rights, the rule of good situation and the rule personal and collective legitimate interest protected by law, the procedure of init...

我国采用民商法中诚实信用原则、禁止权利滥用原则、公序良俗原则以及国家、集体和个人合法权益受法律保护原则作为解决公司法人人格否认制度的法律适用问题。因此，有必要进一步对诉讼主体———原告、被告的提起和追加程序；举证责任倒置的原则；公司法人人格被否认后应由被否认人格的公司及其股东对债权人承担无限连带责任等等适用问题作出全面探讨。

Method8: The paPef, fOr the first time, studied the apoptosis or necrosis of K562cclls induced by bungarus multicinclus crude venom and several components frOmcrude venom by cation exchange chromatography\' through the quatitative analysis offlow cytometry as well as the qualitative analysis, such as inversed opticalmicroscopy, MTT method and fluorescent microscopy, and so on.

本卖验采用羧甲基纤维素C-52阳离子交换层析将银环蛇粗毒进行了若干毒素组分的分离纯化，并以流式细胞术定量分析为主，以倒置显微镜、MTT比色法、荧光显微镜等多种定性分析为辅，首次对银环蛇粗毒及其若干组分蛋白对人慢性粒细胞白血病K562肿瘤细胞的细胞杀伤作用和凋亡诱导情况进行了研究。

Methods Human PDLC cultured in vitro was collected and seeded on Col, HA, HA/Col scaffolds crosslinked by carbodiimide. The influences of scaffolds on cell adhesion and growth were observed by MTT assay. The growth of human PDLC on scaffolds was observed through inverted phase contrast microscope and scanning electron microscope.

将体外培养的人牙周膜细胞接种到碳化二亚胺交联的胶原、透明质酸及透明质酸/胶原支架上；MTT法检测支架对人牙周膜细胞黏附、生长的影响；并用倒置相差显微镜和扫描电镜观察形态变化。

The expression levels of Bcl-2 protein were assayed by Western-blot and cell proliferation was measured by MTT method.

通过倒置荧光显微镜和流式细胞仪观察细胞的转染效率，Western印迹法检测Bcl-2蛋白表达水平，MTT法测定细胞增殖情况。

MethodsHuman embryonic lens epithelial cells were cultured by the explanting culture methods. An inverted phase contrast microscope, electron microscope and immunocytochemistry were used to study the growth specialties of the human embryonic lens epithelial cells.

方法组织块贴壁法培养人胚晶状体上皮细胞，通过倒置相差显微镜、透射电子显微镜，采用免疫细胞化学法分析人胚晶状体上皮细胞的生长特性。

METHODS The killing effects of NDV-L on cancer cells and normal cell were observed by means of plaque assays, HA titer test and MTT test. The inducing effects of NDV-L on apoptosis were observed by means of Giemsa's dying test, phase contrast light microscopy, transmission electron microscope, DNA extraction, and flow cytometry.

通过噬斑形成单位、HA效价、MTT法等方法检测NDV -L对细胞的杀伤作用；通过Giemsa's染色法、倒置相差显微镜、透射电镜、DNA凝胶电泳、流式细胞仪检测NDV -L诱导肿瘤细胞凋亡的作用。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。