体外发育的

The three types of reconstructed embryos(reconstructed embryo of goat-rabbit, goat-bovine and goat-goat) were produced respectively by nuclear transfer using goat ear firbroblast cells as donors and rabbit, bovine and goat ooctyes as recipients, and the factors of cytoplast environments influencing cloned embryo development in vitro, cytoplast effect in nuclear transfer, the methods of transferring cloned embryo and the effects of pregnancy factor on implantation of cloned embryo were studied in this experiment, in order to supply basis for solving the difficulty of implantation of inter-species cloned embryo in recipient and improving the efficiency of cloning.

本试验以波尔山羊耳成纤维细胞为供体，分别以牛、羊、兔的卵母细胞为受体进行体细胞核移植，构建了山羊-兔、山羊-牛和山羊-山羊三种重构胚。研究了不同的胞质环境对重构胚体外发育的影响与核移植中的胞质效应，并对异种克隆胚胎采用了去透明带移植和配种后再移植的方法，探讨移植方法和妊娠因子对异种克隆胚胎在受体动物体内发育的影响，旨在为解决异种克隆胚移植不易着床发育的难题和为提高动物克隆效率提供理论依据。

The results shown as follow: 1. The study on mice denuded oocytesin vitro maturation, in vitro fertilizationand in vitro development. This part of paper is to discuss whether the remove of granulose cells can affect the mice oocytes maturation rates, fertilization rates and development rates in vitro.

一、去除卵丘细胞的小鼠卵母细胞的体外成熟、体外受精初步研究本实验探讨了不同成分明确的成熟培养液对去除卵丘细胞的小鼠卵母细胞的体外成熟、体外受精和胚胎的体外发育的影响。

Studies on mouse nuclear transfer with compacted morula blastomeres as donors In this study, C57BL/6 mouse morulae blastomeres and Kunming white mouse MⅡoocytes were used as donors and recipients, respectively, to investigate the effects of different sucrose concentration on the manipulation time of nuclear transfer, and the in vitro development of reconstructed embryos.

以致密桑椹胚卵裂球为供体克隆小鼠的研究该研究以C57BL/6 小鼠致密桑椹胚卵裂球为供体、昆明白小鼠MⅡ期卵母细胞为受体，研究了不同蔗糖浓度对核移植操作时间及重构胚体外发育的影响。

To understand the factors effecting on mammalian early embryo development, we carried out the study through 3 points of view, oocytes, zona and embryo culture in vitro.

本研究分别从卵母细胞、透明带以及胚胎体外培养三个角度来探讨影响哺乳动物早期胚胎体外发育的因素。

To optimize the in vitro culture system of mouse pronuclear zygote and to investigate the effect of FBS, PVA, hLIF on mouse zygote development in vitro, we did four experiments separately.

筛选最佳的小鼠胚胎体外发育培养体系，并进一步探讨不同浓度的FBS、PVA、hLIF对其体外发育的影响，从而为小鼠卵母细胞核移植后重构胚的体外培养提供支持。

Then we compared cleavage rate and blastocyst rate among different groups. After that we evaluated the quality of blastocysts by using ICM/TE (Inner cell mass/Trophectoderm cells) ratio via staining with propidium iodide and Hoechest333258, and analyzed the level of genome methylation and histone acetylation by immunofluorescence.

比较各组受精卵的卵裂率和囊胚发育率；并应用碘化丙啶和Hoechest333258对囊胚进行染色，利用ICM/TE值评价各组胚胎体外发育的质量；同时将囊胚进行免疫荧光染色，观察其基因组甲基化和组蛋白乙酰化的水平。

Tolerance threshold of 2-cell mouse embryos to ethanol was around 1.5% when these embryos were cultured in Whitten's medium with 0, 0.1%,0.5%,1.0%, 1.5%, 2.0%, 3.0%, 5.0% and 10.0% ethanol, respectively.

用含不同浓度乙醇的Whitten氏培养液对小鼠2细胞、4细胞、8细胞和桑椹期胚胎分别进行体外培养，研究了乙醇对小鼠不同发育时期胚胎体外发育的影响。

The Effect of Some Environmental Factors on the Development of Mouse Embryos In Vitro

不同环境因素对小鼠胚胎体外发育的影响

Objective To observe the effects of coculture of mouse embryo with endometrial epithelial cells on early embryonic development in vitro.

目的：采用子宫内膜上皮细胞与小鼠胚胎共培养，观察其对早期胚胎体外发育的影响。

Methods Mouse endometrial epithelial cells were isolated and cultured,then it was cocultured with mouse embryos fertilized in vitro.

目的：采用子宫内膜上皮细胞与小鼠胚胎共培养，观察其对早期胚胎体外发育的影响。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。