产生诱导作用的

In the latter generations of rice suspension cell, the activity of extracellular POD and the cell suspensibility curve raises at first and then declines. The activities of extracellular POD induced by exogenous H_2O_2, CaCl_2 and VK3 are different and the same concentration of VK3, CaCl_2, H_2O_2 can all inhibit extracellular POD activities, especially to the callus tissue subculturing for six times.

外源H_2O_2，CaCl_2与VK3诱导悬浮细胞所产生的胞外POD活性并不相同，相同浓度的VK3、CaCl_2和H_2O_2都抑制胞外POD活性，对继代六次的愈伤组织的悬浮细胞胞外POD活性影响最大，H_2O_2对POD的抑制作用更为强烈。H_2O_2随浓度和处理时间的增加，使胞外POD的活性减弱；处理6h、12h，胞外POD活性随着VK3和CaCl_2浓度的增加而增强，但处理48h胞外POD活性随浓度的增加而减弱。

The cooperation of 8-MOP/UVA could induced dermis of the non- lesion back skin of vulgar psoriatic patients and the cultured dermic fibroblasts produced large aging-associated mutations of mtDNA rapidly, it is feasible to establish human models in vivo and in vitro which could produced large aging-assoiated mutations of mtDNA rapidly for studies of the mechanisms of mutations of mtDNA in skin photoaging.

2.8-MOP/UVA可诱导寻常型银屑病患者背部非皮损区真皮组织及培养的成纤维细胞迅速产生光老化相关的mtDNA突变，建立迅速产生光老化相关mtDNA突变的体内外模型，利用该模型进一步研究mtDNA突变在光老化皮肤中的产生机制及其在皮肤光老化形成中的作用是可行的。

These results indiacated that tranferring macrophages combinedwith cytokines could be a new adoptive immunotherapy protocol foradvanced cancer,and rTNF used in vivo locally could induce andactivate macrophages to kill tumor cells.Monocytes when activatedunderwent a series of phenotypic and functional changes includingthe expression of IL-2R which may provide an important immunoregu-latory pathway.The presence of lectin-like molecules on thesurface of monocytes and tumor cells may bring theeffector/target cells together,thus facilitating the inductionof apoptosis in target cells by triggering the production ofcytolytic factors and the modification of target of targer cell surface anti-gens.(such as HLA-DR).

综合以上结果可以得出以下结论：转输巨噬细胞并结合细胞因子的联合应用是肿瘤继承性免疫中的又一新的、有潜力的方法；抗肿瘤细胞因子rTNF可通过激活巨噬细胞在体内抑制对其不敏感的肿瘤细胞的生长；人单核细胞在一定条件下激活后，可表达IL-2R，进而一方面增强其自身对IL-2的敏感性，另一方面在体内也具有调节T细胞功能的作用；单核细胞和肿瘤细胞上存在的凝集素类受体可促使两种细胞之间在凝集素介导下相互接近，并诱发单核细胞产生细胞毒因子，以及通过调节靶细胞上表面抗原的表达，促进诱导靶细胞的细胞凋亡。

Reactive oxygen species causing DNA oxidative damage comes from two kinds of ways:one is from cellular normal physiological metabolism;the other is from outer environment.Redox-sensitive green fluorescent protein was expressed in Saccharomyces cerevisiae.Recombinant cells were evaluated in monitoring the changes in the redox state of living cells when challenged with toxicologically relevant metal ions NaAsO_2 or Pb(NO_3)_2 by measuring emission intensity at 510 nm with a Hitachi F6500 fluorescence spectrophotometer,roGFP expressed in yeast responded not only to typical membrane-permeant oxidants H_2O_2 and reductants DTT,but also to toxicological metal ion-induced intracellular redox changes in a dose-dependent manner.Moreover,exposure of yeast cells to NaAsO_2 or Pb(NO_3)_2 at concentrations that induced redox changes reported by roGFP caused up to 2～3 fold increases in DNA mutation frequency.This mutagenic effect was largely caused by oxidative stress since blocking the production of hydryl radicals with thiourea significantly reduced the mutation rate as well as delayed the cell death.

本文将对氧化还原状态变化敏感的绿色荧光蛋白roGFP1-R12，在酵母细胞中实现了多拷贝强表达；荧光扫描经强氧化剂H_2O_2和还原剂DTT以及环境中重金属NaAsO_2或Pb(NO_3)_2处理后的酵母细胞悬液，测定510 nm处的荧光发射强度结果显示，表达的绿色荧光蛋白对氧化还原水平敏感，且在510 nm处的荧光强度与一定的重金属浓度呈正相关，即roGFP1-R12在510nm处的荧光发射值随重金属浓度的增高而增强，从而说明重金属对细胞的毒性在一定程度上很可能是通过破坏细胞内的氧化还原平衡发生作用；同时通过该绿色荧光蛋白对胞内氧化还原状态变化的响应情况可以来实时检测环境中的重金属；遗传学的点突变频率及致死率实验数据表明，重金属能导致菌体的点突变频率和致死率升高，且活性氧的清除剂巯基脲能明显降低这种点突变和致死率，说明由重金属引发的这种点突变和致死效应在很大程度上是依赖于重金属对细胞诱导产生的氧化胁迫。

The protection of AF－1 was observed with the measurement of transudatory rate of LDH ,the breaking rate of DNA and contents of LPO and SOD,Results:The intotoxincation of glutamate was decreased by AF－1 which reduced the transudatory rate of LDH,the breaking rate of DNA and the content of LPO,and increased the content of SOD in culture significantly.

通过测定乳酸脱氢酶漏出率、DNA断裂率、超氧化物歧化酶和脂质过氧化物，观察AF－1药物的保护作用。结果：高浓度的谷氨酸可增加培养神经细胞的LDH释放率和DNA断裂率，并诱导自由基产生。AF－1明显降低谷氨酸的神经细胞毒性，使LDH释放率及DNA断裂率明显降低，LPO含量明显下降，SOD含量明显升高。

Compared to the control, the Rosachinensis var floribunda treated with CaZ+and SA maintain higher levels of antioxidantenzyme activeties, inhibited the electrolytic leakage of membrane, and diminished thecontent of MDA in Rosa chinensis var floribunda under high temperature stress.

近年来，由于&温室效应&的作用，全球气候逐渐变暖，高温天气的出现同益频繁，高温对植物的生长和发育产生了许多不良的影响。所以研究高温胁迫对丰花月季生理效应和耐热性诱导具有现实意义，为丰花月季的引种栽培及生理效应提供参考。

XIAP expression could not be affected by the apoptotie inductions mediated by death receptor and mitochondria pathway in both cell lines.

通过对肾癌组织和细胞的研究，提示抗凋亡的XIAP在肾癌组织中呈肿瘤进展依赖性增高、在抗凋亡的肾癌细胞高表达，在肾癌细胞抗凋亡机制中发挥重要的作用，影响肾癌对凋亡信号的敏感性，使肾癌细胞获得生存发展的优势，从而促进肾癌的进展，并产生对诱导凋亡药物的抵抗；应用基因克隆和Tet-Off基因可控性表达系统技术构建的肾癌细胞XIAP稳定表达细胞系，对深入研究XIAP在肾癌抗凋亡中的机制，具有实用意义。

The stimulatory effect on the MMP-13 promoter was partially inhibited by mutation of the osteoblast-specific element 2 (OSE-2) binding site ⑸ Core binding factor-1 (Cbfa-1) expression peaked at 7 days ⑹ of differentiation, and was phosphorylated by PKG in itro.

免疫组织学检查揭示在17天大的embryo iNOS缺乏的老鼠MMP-13和Cfba-1表达水平均降低。Cfba-1 mRNA的沉默阻滞MMP-13的表达，不影响内源性的NO产生，肯定了他在MC3T3-E1细胞中NO诱导的MMP-13的表达中的作用。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。