两月一次的

In the model of tumor cells metastases with cell line A673, 10〓 tumor cells can be detected after only one ampliation with outer primers, which means we can find 1 tumor cells in 10〓 normal PBMC, and enlarged again with inner primers, 10〓 tumor cells can be detected, which means we can find 1 tumor cell in 10〓 normal PBMC. In the simulative model of tumor cells metastases with RNA, 10〓 tumor cell can be detected that is to say we can find 1 tumor cell in about 10〓 normal PBMC. In all 15 samples of 11 patients, 2 patients'EWS/FLI1 expressions were negative, no evidence of metastases was found followed up by 8 months and 30 months respectively. Semi-quantitative study was used to detect the expression level of EWS/FLI1 mRNA in the other 9 positive patients, and metastases occurred in 6 high-level expression patients in 2-24 months after the operation, no evidence of metastases in the other 3 low-level expression patients up to now.

应用A673细胞系建立的肿瘤转移模型，单独应用外引物进行一次扩增，可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；而在此基础上再次应用内引物扩增，则可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；在用RNA模拟的肿瘤细胞转移模型中，两次扩增可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞。11例患者的15份标本中，2例没有检测到EWS/FLI1 mRNA的表达，分别随访8个月和30个月，没有发现临床转移的证据；对其余9例阳性表达的病例进行半定量的研究，6例EWS/FLI1 mRNA高表达的病例，分别在术后2-24个月发生转移，而3例EWS/FLI1 mRNA低表达的病例，则至今没有发现转移的证据。

I am not the beekeeper of the family -- my wife and two of my kids handle that role -- but I've learned some surprising things in the past two or three years.

但在过去的两三年中我了解了蜜蜂身上一些让人惊讶的东西。夏季蜜蜂的寿命平均为六个月。想想看，那意味着每两个月蜂巢就会完成一次自我复制。

I feed brine shrimp flake at least once a day and usually frozen brine shrimp.

我至少每天投喂一次薄片和冰冻丰年虾，偶尔也会喂血虫和糠虾，一个月投喂两次活的丰年虾。

I handed him a cigaret in good time to ease the atmosphere and chatted with him intermittently. He said angrily this thing is purely scam, but the station arranged for him to engage in this, he has no other choice, next month he mustn't take this kind of business. Who sells the ads directs on his own. He continued to confide that it's alright to call someone for one or two times, if more times or they learn what matter it is, they are unwilling to cooperate. This involved him in a lot of gossip.

我适时的递上一支烟，缓和了下气氛，和他断续的聊着，他气愤说这个东西纯属骗子，台里安排他搞这个，没有办法，下个月他一定不接这种业务了，谁拉的广告谁自己来导播，他继续和我诉说着打电话给人家配合，一次两次还好，多了或者别人知道是个什么事情了，就不愿意配合，搞的他还听了好多闲话。

Personally think that the first time in his place before the days of the best things to stay if not used, because two can play much on the attack, he had little chance to moonset, especially in such a restricted Wudang martial skills and more, so he used Pegasus飞瀑second, if spent immediately, this time also on the cooling down, and whether or not he did not bite on the run are chasing him, and if the time spent immediately on a drop-off, this time the situation must be cut West on the use of cooling, if not cooled quickly to their own on a few fresh, so down, it is estimated that more hope to win big.

个人认为在他放第一次天外之前最好别用呆若，因为两个远攻的打能放到他月落的机会不多，尤其是武当这样受制技能多的门派，所以等他用完第二次天马飞瀑，就马上呆若，这个时候月落也该冷却了，不管他上没上当都追着他跑，等呆若时间一过就马上月落，这个时候要是斩情决冷却了就用西子，要是没冷却就赶紧给自己清新几下，这样下来，估计赢的希望就比较大了。

Noviciate Salary is RMB800 net per month and according to your performance to promote RMB100 per 2 month until you graduated from university.

实习期净工资为800元一个月，并且会根据你的表现每两个月提升工资一次，每次提升为200元，直到你从大学毕业。

Similar experiments, but using Chemical Release Modules, have been launched from Churchill, Manitoba. In 1980, Brian Whelan's "Project Waterhole" disrupted an aurora borealis, bringing it to a temporary halt. In February 1983, the chemical released into the ionosphere caused an aurora borealis over Churchill. In March 1989, two Black Brant X's and two Nike Orion rockets were launched over Canada, releasing barium at high altitudes and creating artificial clouds.

同样的但是采用化学释放模型的实验，在加拿大马尼托巴的丘吉尔被启动。1980年，布瑞安威兰的"水洞项目"中断了一次北极光，使之暂时停止。1983年2月，排放进电离层的化学物质引发了丘吉尔上空的北极光。1989年3月，两艘"黑雁X"和两艘"耐克奥瑞恩"火箭在加拿大上空被发射，在高纬度释放钡产生人工云。

Gangsters then find rich pickings in nobbling the participants. In November the European football authority, UEFA, launched a big inquiry involving 200 matches and teams from 11 countries. In February it banned a Bosnian referee for life and suspended two other officials.

这之后地痞流氓开始收买相关人员，同时给自己捞一大笔不义之财。11月，欧足当局——欧洲足球联盟，对来自11个国家的200支队伍及其之间的比赛进行了一次地毯式的调查。2月，一波斯尼亚籍裁判被终生禁止裁判工作，另有两名官员被停职。

It's the bloodiest attack in two months. Two car bombs ripped through a crowded Baghdad market.

这是两个月以来最残酷的一次袭击，两次汽车爆炸事件摧毁了巴格达一个繁华的市场，并造成至少88人死亡。

KEN NOGUCHI: You carry the garbage from eight thousand meters down to six thousand meters, again and again for two months, it's really hard especially when temperatures are up and there is one avalanche after another.

野口健：在两个月里，需要一次又一次地将垃圾从八千米的高度带到六千米的高度。当温度升高时，这个工作特别困难，因为雪崩一个接着一个。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。