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The composition of amino in feed matter and feed draff after degradation for sixteen in rumen compared, from which we found the content of vary of amino acids in peanut meal and Soybean meal had increasing trends after sixteen hours cultured in rumen. The increasing trends of leucine, isoleucine, threonine, phenylalanine were greater. The increase trends of lysine, methionine, arginine and histidine were smaller. The content of varies of amino acid in cotton seed meal matter had decreasing trend s after sixteen hours cultured in rumen, but decreasing value was small. The compositions of amino acid in lees and rape seed meal matter were steady after sixteen hours cultured in rumen.

通过把饲料原料中氨基酸的组成和瘤胃降解16h后饲料残渣的氨基酸组成比较发现各种蛋白质饲料瘤胃降解前后氨基酸组成均发生了一定的变化,总的来说,氨基酸浓度在降解后有上升趋势(p<0.05)的有亮氨酸、异亮氨酸、苯丙氨酸、缬氨酸、苏氨酸、丙氨酸和脯氨酸,氨基酸浓度在降解后有下降趋势(p<0.05)的有赖氨酸、精氨酸、谷氨酸和胱氨酸,氨基酸浓度在降解后差异不显著的有组氨酸、天门冬氨酸、甘氨酸,降解后无明显规律的有蛋氨酸、丝氨酸和酪氨酸。

The results showed:among the 7 isolates, five isolates of HY3、GY1-3、ZJ1-1、HP1、FC3 had same colony shape, irregular shape, liquidlike, slimy, opacity with smooth surface;the other two isolates had same shape, irregular shape, dry, opacity with coarse surface. By inoculating eucalyptus with the 7 isolates, the plants were infected apparently, and the young plants of eucalyptus in control experiment with tap water were not infected. By cultivating eucalyptus cuttings with the bacterial suspensions without EPS, the incidence of disease was very distinct,but compared with the former bacteria suspension,the incidence of disease has decreased at different degrees. By screening out two isolates of strong pathogenicity and two isolates of weak pathogenicity from the 7 isolates,making the bacterial suspensions with them to inoculate the young plants of eucalyptus, two treatments of cutlings and ramets with rats were set with 5 repetitions in every treatment, the results of data analysis showed: for the cutlings, the bacterial contents in upper and middle parts、upper and lower had significant difference;for ramets with roots, the bacterial contents in upper, middle parts, lower had significant difference between each other; For both the cutlings and ramets with roof, the bacterial contents in xylem and phloem had significant difference. The interaction between vertical and horizontal parts for the bacterial content had significant difference. For the two isolates of HY3 and 93B which were screened out at last,their activities of the cellulase were: 1.955ug/ and 1.288ug/ respectively, and had significant difference; the activities of pectase were: 1.325 ug/and 1.24ug/ respectively, and had no significant difference. The content of EPS extracted from the two isolates of HY3 and 93B was very different: 7.08x10-8ug/cell and 5.17x10-8ug/cell.

结果显示:7个菌株中,其中5个菌株HY3、GY1-3、ZJ1-1、HP1、FC3的菌落形态相同:不规则形状、流体、粘性、不透明、表面光滑;另外2个菌株93B、GN1菌落形态相同:不规则形状、干燥、不透明、表面粗糙;用7个菌株接种剪根桉树苗,发病情况非常明显,而自来水对照实验中桉树苗却不发病;无EPS菌悬液培养桉树剪根苗,发病率也很明显,但是相比原菌液,则发病率有不同程度的下降;从7个菌株中间筛选出来2个强致病性菌株和2个弱致病性菌株,用它们配制菌悬液培养桉树苗,设置剪根和不剪根两个处理,每个处理设置五个重复,数据分析结果显示:对于剪根苗,上部和中部、上部和下部的含菌量有显著的差异,中部和下部含菌量差异不显著;带根苗,上部、中部、下部含菌量彼此之间差异显著;不管是剪根苗还是带根苗,木质部和韧皮部含菌量之间的差异都非常显著;上中下与木韧交互作用中,含菌量差异非常显著;最后筛选出来的强弱2个菌株HY3和93B,它们的纤维素酶活性分别为:1.955ug/和1.288ug/,具有显著的差别;果胶酶的活性分别为:1.325 ug/和1.24ug/,没有显著的差别,而且HY3和93B两个菌株细胞分泌的胞外多糖的含量差异很显著,分别为:7.08×10-8ug/cell和5.17×10-8ug/cell。

Along with seawater stress strength upward, the ratio of root to shoot, Na+, Cl- and soluble sugar contents were increased significantly. Under the 10% and 20% seawater treatments, chlorophyll contents have no obvious difference compared with the control, but significantly decreased when the seawater stress further increased. Under the 10% seawater treatments, K+ contents in the shoot and root of Lactuca indica seedlings have no distinct difference compared with the control, but along with seawater stress strength upward, K+ content were markedly decreased by degrees. However, soluble protein content first increased then decreased with seawater concentration augment.

结果表明:苦荬菜幼苗地上部受海水胁迫较为显著,而根在30%海水以内与对照相比没有显著差异;根冠比随着海水比例的增加而提高;10%和20%海水处理下,叶绿素含量与对照相比差异不显著,但随着海水浓度的进一步增加,叶绿素含量显著下降;10%海水处理下,苦荬菜地上部分及根K+含量与对照差异不显著,高于10%海水处理,K+含量随海水比例的增加地上部和根部均逐渐显著降低;海水处理下,体内Na+和Cl-含量逐渐显著增加;地上部可溶性糖逐渐显著增加,而可溶性蛋白呈先升后降。

The increased amount of non--exchangeable NH4^+-N during the incubation indicated as the following tendency, namely, Eum-Orthic Anthrosols 〉 Los - Orthic Entisols 〉 Hap-Ustic Isohumisols 〉 Ust-Sandiic Entisols. Organic material influenced significantly the increased amount of non-exchangeable NH4^+-N (P was 0.0002, 0.0004 and 0.0003 when incubated at 20 d, 50 d and 60 d, respectively). NH4^+-N increased remarkably when Stlpa bungeana and Medicago sativa were added compared with no addition. The contents of non-exchangeable NH4^+-N increased significantly when added (NH4)2SO4 compared without addition at 20 d, 40 d and 60d of incubation and P was 0.0037, 0.0033 and 0.0027, respectively. It was the result that the NH4^+-N from (NH4)2 SO4 was fixed within the soil. The increased amount of non-exchangeable NH4^+-N increased significantly for different soils, on which different vegetation types grew before the collection of soil samples, when incubated at 20 d (P=0.0434), but not significantly at 40 d and 60d (P=0.7378 and 0.5375). The increased amount of non-exchangeable NH4^+-N in the soil, on which crop straw and nitrogen fertilizer had been incorporated for a long-term period, was larger than that of no addition, but it was not significantly different among these two fertilization models. Soil clay, total N and organic matter were positively correlated remarkably with the contents of non-exchangeable NH4^+-N, the increased amount of non-exchangeable NH4^+-N had no correlation with soil clay, whereas it had significantly positive correlation with total N and organic matter.

培养期间非交换性NH4^+-N的增加量均表现为以土垫旱耕人为土最大,其次是黄土正常新成土,简育干润均腐土和干湿砂质新成土较小;添加有机物料极显著影响培养期间的非交换性NH4^+-N增加量(培养20d、40d和60d时P分别为0.0002,0.004和0.0003),表现为紫花苜蓿和长芒草土壤非交换性NH4^+-N的增加量均极显著高于不添加有机物料的对照土壤;在培养20d、40d和60d时,加(NH4)2SO4土样非交换性NH4^+-N的增加量显著大于不加(NH4)2SO4土样(户分别为0.0037,0.0033和0.0027),这是土壤对(NH4)2SO4中NH4^+-N固定的必然结果;不同植被类型土壤培养20d时的非交换性NH4^+-N增加量差异显著(P=0.0434),培养40d和60d时差异不显著(p分别为0.7378和0.5375);长期秸秆和氮肥配施土壤非交换性NH4^+-N增加量大于不施肥对照土壤,但差异不显著土壤黏粒、全氮和有机质与培养0d、20d、40d和60d时土壤的非交换性NH4^+-N含量均呈极显著正相关;而非交换性NH4^+-N的增加量与粘粒无相关性,但与全氛和有机质呈显著正相关。

The fiber length has only little influence on the basic density within the growth rings, and significant correlation at 0.01 levels was found between the basic density and the fiber length among the different rings. Only slight negative correlation was found between the basic density and the fiber width within the growth rings, but significant positive correlation at 0.01 levels was indicated between the basic density and the fiber width among the growth rings, contrary to that of fiber length. It was demonstrated that significant positive correlations at 0.01 levels between the basic density and fiber double wall thickness, fiber length to width ratio and double wall thickness to diameter ratio, significant negative correlations at 0.01 levels between the basic density and fiber diameter and diameter to width ratio, only slight negative correlation between the basic density and fiber width both in the same growth rings and among the different growth rings. No significant correlation was found between the basic density and the vessel morphological features, nor was the tissue proportion in the same growth rings. But among the different rings, it was found there was significant positive correlation at 0.01 levels between the basic density and the fiber proportion among the different rings, and significant negative correlation at 0.01 levels between the basic density and vessel-elements proportion and ray proportion, only slight negative correlation between the basic density and the parenchym proportion. Significant or no significant negative correlation was found between the basic density and the microfibril angle in the same growth rings, but significant negative correlation was found between the basic density and the microfibril angle among the different growth rings.

生长轮内纤维长度对基本密度的影响不大,而在不同生长轮间纤维长度与基本密度达极显著正相关,纤维宽度与此相反,同一生长轮内纤维宽度与基本密度极显著负相关,不同生长轮间只有微弱负相关;基本密度与纤维双壁厚、长宽比、壁腔比在生长轮内和生长轮间均呈极显著正相关,而与胞腔直径、腔径比均呈极显著负相关,仅与纤维宽度呈微弱的负相关;导管形态对基本密度的影响不显著;同一生长轮内组织比量对基本密度的影响也不显著,但不同生长轮间基本密度与纤维比量呈极显著正相关,与导管比量和木射线比量呈极显著负相关,与轴向薄壁细胞比量仅呈不显著负相关;生长轮内基本密度与微纤丝角呈显著或不显著负相关,但在生长轮间这种负相关达到极显著水平。

The result shows that: a positive correlation is found between the amount of application of chemical fertilizer and some factors, such as farming hours, attitude towards risks, total family incomes and the proportion of agricultural labour and non-agriculturallabour. A negative correlation is found between the amount of application of chemical fertilizer and other factors, such as farmers' educational background, their cognition of chemical fertilizer, and the channel of information. The agricultural incomes and planting areas seem to be not notable inflecting factors.

研究结果表明:户主的从农时间、风险态度、农户家庭总收入、以及劳力与非农比例情况对农户施肥量成正向相关关系;户主的教育程度、农户对于化肥的认知情况、信息渠道、施肥方式、是否施用有机肥、农户对于化肥利用率的认知以及农户对于污染认知程度等因素对农户施肥量成负向相关关系;农业收入和种植面积的影响则是不显著的

Analysis of the relation between serotonin and its receptor in OFC and stress depression(n=8):(1) Microinjection of 5-HT into OFC significantly shortened immobility time, dejections have no great changes; though locomotor、 grooming activitives have little increased, they have no significant difference; rearing scores greatly reduced;(2)microinjection of pCPA into OFC significantly prolong immobility time, dejections have no great changes; locomotor、rearing activities are greatly reduced, but have no significant effect on grooming activity receding;(3)compared with the effect of 5-HT alone, microinjection of spiperone combined with 5-HT into OFC, significantly prolong immobility time and greatly reduced dejections; locomotor、 rearing、 grooming activities are all reduced, but have no significant differences.

眶额叶区5-羟色胺(5-hydroxytryptamine,5-HT)及其受体与应激性抑郁症的关系(n=8):(1)注射5-HT后,大鼠FST不动时间明显降低(P<0.05),粪便数量变化不显著(p>0.05),OFT水平运动、修饰得分虽均有升高,但无显著性差异(P>0.05),垂直活动显著降低(P<0.05);(2)注射色氨酸羟化酶抑制剂-对氯苯丙氨酸后,FST不动时间明显增加(P<0.01),粪便数量无显著性变化(p>0.05),OFT水平、垂直运动显著减少(P<0.05),修饰降低但无显著差异(P>0.05);(3)与5-HT组比较,先注射5-HT_(1A)受体拮抗剂后再注射5-HT,FST中的不动时间明显增加(P<0.01),粪便数量显著减少(p<0.01),但OFT水平、垂直、修饰活动均有所减少,却无显著性差异(P>0.05)。

Every community was stable relatively. Interspecific association was the loosest and stability was the highest in Q. wutaiensis community. But the insignificant positive association among species showed that Q. wutaiensis community has not reached the stable state, so we still should protect the local natural vegetation reasonably for a long time.

演替系列各群落都具有相对的稳定性,演替顶极辽东栎群落的种间联结性最弱,稳定性最高,但多物种间不显著的正相关说明该地区分布最广、面积最大的辽东栎群落可能尚未达到稳定状态,仍需要对该地区天然植被进行长期保护。

When aborted rats were injected with 10 IU/g IFN-γin group D, compared with group A, expression of TNF-αinmmuopositive substance in the nucleus pre-opticus medialis, nucleus supra-opticus, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus paraventricularis hypothalami, nucleus arcuatus hypothalami were highly increased, conversely, the ones in the nucleus pre-opticus suprachiasmaticus, neurohypophsis, adenohypophysis, ovary were obviously decreased, the change in the uterus was not remarkable; Compared with group B, expression of TNF-αin the arcuatus hypothalami was obviously enhanced, while in the nucleus pre-opticus suprachiasmaticus, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus paraventricularis hypothalami, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, neurohypophsis, pars intermedia, ovary, the results were on the contrary, the uterus had no significantly variation; Compared with group C, expression of TNF-αin the nucleus pre-opticus medialis, nucleus supra-opticus, nucleus paraventricularis hypothalami, nucleus arcuatus hypothalami, adenohypophysis and pars intermedia had an obviously risen trend, however, the variation in the nucleus pre-opticus suprachiasmaticus, nucleus periventricularis hypothalami, neurohypophsis, pars intermedia, follicle and corpus luteum was completely opposite, uterus had no obviously change.

外源腹腔注射10 IU/g IFN-γ后,同A组相比:D组TNF-α免疫阳性产物在下丘脑视前内侧核、视上核、视前大细胞核、室周核、室旁核、弓状核显著增高,在视交叉上核显著降低,在其它核团较A组无显著差异,在神经垂体、垂体前叶、卵巢中阳性产物表达均较A组显著降低,在子宫中的表达较A组变化不明显;同B组相比:D组TNF-α免疫阳性产物在弓状核显著增高,在视交叉上核、视前大细胞核、室周核、室旁核、腹内侧核、背内侧核、神经垂体、垂体中间部、卵巢各部阳性物质表达均显著降低,在子宫表达变化不显著;同C组相比:D组下丘脑视前内侧核、视上核、室旁核、弓状核、垂体前叶与中间部均显著增高,在视交叉上核、室周核、神经垂体、卵泡、黄体中均显著降低,子宫中表达变化不明显。

Results were shown as followings:(1) Sodium selenite at 0~2.5 μmol/L significantly increased the antioxidative capacity of L-02 cells without having remarkable impact on SMMC-7721 cells;(2) Sodium selenite at concentrations above significantly increased telomerase activity, hTERT gene expression and telomere length of L-02 cells without significant impact on SMMC-7721 cells;(3) Sodium selenite at higher concentrations (larger than 5 μmol/L) resulted in peroxidation of L-02 cells, while scutellarin significantly counteracted its effect;(4) Selenium-rich amino acids from silkworm pupas in the range of 0.5~2.5 μmol/L Se significantly inhibited SMMC-7721 cell growth, induced apoptosis and cell cycle change, and the generation of reactive oxygen species. In contrast, sodium selenite and selenomethionine only had weak impact on them at the same concentrations;(5) A new selenium-containing protein was found from selenium-rich silkworm pupas, which is worthy to be study further;(6) An expression vector containing ansense RNA of hTERT gene were constructed and used to transfect SMMC-7721 cells. They were observed to inhibit hepatoma cells.

结果如下:(1)0~2.5μmol/L亚硒酸钠显著性增强L-02细胞的抗氧化能力;而对SMMC-7721细胞的作用不显著;(2)该浓度硒显著性提高L-02细胞端粒酶活性、增强hTERT基因表达和延长细胞端粒长度;而对SMMC-7721细胞的作用均不显著;(3)高浓度硒(5μmol/L以上)显著性抑制L-02细胞生长、致细胞过氧化,灯盏花素能拮抗硒所致过氧化、降低硒毒性;(4)0.5~2.5μmol/L富硒蚕蛹氨基酸显著性抑制肝癌细胞SMMC-7721生长、导致细胞凋亡和周期改变、诱导细胞产生活性氧,同浓度亚硒酸钠和硒代蛋氨酸对其抑制不显著;(5)富硒蚕蛹蛋白经分离纯化和鉴定后发现存在一新含硒蛋白,其结构和功能有待研究;(6)通过已有的含hTERT基因质粒,成功构建hTERT反义RNA表达质粒,转染SMMC-7721细胞后对其生长具有抑制作用。

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Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克,果实色泽鲜艳,汁多味浓,甜酸适口,离核仁甜,清末时就驰名陕、甘两省,清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异:红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感,ED90值分别为47.65、71.67和29.17g/hm2;春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感,ED90值分别为96.91、114.20g/hm2;而番茄不敏感。

However, I have an idea.

不过,我有个主意。