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objective to explore the best method of nursing in laparoscopic operation of subtotal hysterectomy with pk scapel.methods ninety seven patients under going operation of laparoscopic subtotal hysterectomy with pk scapel were slected.the bleeding volume,operation time and methods of nursing including machine check,proceeding and maintenance of instruments.results bleeding volume was lessened significantly and good nursing during operation can improve safety and success rate of losh.conclusion good nursing with well knowledge of pk scapel,accurate proceeding and sterilization and skilled cooperation plays a key role in assurance of the success of losh.

摘 要］目的：探讨在腹腔镜下使用pk刀行子宫次全切除术配合的方法。方法：对97例使用pk刀在腹腔镜下行子宫次全切除术配合进行总结，主要是检查测试仪器功能，按操作程序密切配合手术，术后正确彻底的清洗、保养、消毒与灭菌。结果：在腹腔镜下应用pk刀切除子宫，出血量明显减少，组织损伤小，97例的手术中除1例粘连严重中转开腹外，其余手术均顺利完成，无并发症发生。结论：护士加强对pk刀的应用学习，正确掌握操作规程和灭菌方法，以及娴熟的腹腔镜配合技术是手术顺利进行的有力保证。［关键词］腹腔镜；pk刀；子宫次全切除术；护理配合

Had rarely or never used protective;(11) MiceGrayAuto had realized the automatic processing of video data from the sexual preference observation system of rats, and MiceGrayManu had accomplished the analysis of images of sexual behaviors of rats;(12) Peripheral given of high dose androgen to female rats before its brain sexual differentiation had caused masculinization and defemininization in female rats, therefore led to homosexuality, and based on the results, we had established the female rat homosexual model;(13) Peripheral given of estrogen to male rats before its brain sexual differentiation had little effects on the sexual preference and sexual behavior of adult rats;(14) Peripheral given of estrogen antagonist to male rats before its brain sexual differentiation had markedly reduced masculine sexual behavior and interfered male sexual preference, but had no effect on feminine sexual behavior, and based on such results, we had established the male rat homosexual model, however the model have to be improved for better sensitivity;(15) The volume of sexually dimorphism nucleus of the preoptic area of the high dose androgen-treated female rats was significantly greater, while on the other hand the volume of AVPV of which was significantly lesser, than the female rat control.

并且有17.79％的男性同性恋者在1年内与异性保持着性关系。但在与异性阴道性交时，绝大多数（92.51％）偶然或不使用安全套；(11)MiceGrayAuto软件实现了大鼠性偏爱视频数据自动处理功能，MiceGrayManu软件实验了大鼠性行为图象分析功能；(12)大脑性分化前高剂量的雄激素有促雌鼠雄性化和去雌性化的作用，并表现为同性性倾向，建立了雌鼠同性恋模型；(13)在雄鼠大脑性分化前外周给予雌激素对成年后的性偏爱和性行为未产生明显影响；(14)在雄鼠大脑性分化前外周给予雌激素拮抗剂明显削弱雄性性行为，阻碍了雄性性偏爱，但雌性行为无明显变化，雄鼠的同性恋模型还有待进一步调整和改进：(15)高剂量雄激素处理的雌鼠SDN-POA体积明显大于对照雌鼠组，而AVPV体积明显小于对照雌鼠组。

When the concentration of L-phenylalanine was 6 g/L,the optimum values of volume ratio of organic phase to aqueous phase,reaction temperature,pH value and liquid volume were 1∶1,30 ℃,4.0 and 20 mL/250 mL respectively. Under above optimum conditions,the content of 2-phenylethylalcohol reached 3.0 g/L 48 h later,which increased by about 50% than that in monophasic aqueous system.

当初始底物浓度为6 g/L、水和油酸的相体积比为1∶1、反应温度30 ℃和pH值4.0、装液量20 mL/250 mL 的条件下，转化48 h，产物浓度达到3.0 g/L，比单水相提高了50%。

When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added , kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2－4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L－1 after their number counted.

选取2～5代的小鼠胚胎成纤维细胞，待其至对数生长期倒掉或吸掉培养基，加入含10 mg/L丝裂霉素C的细胞全培养液5 mL，置37 ℃，体积分数0.05的CO2饱和湿度温箱中培养二三小时，在6孔板中加入0.1%明胶包被，放置30 min以上，倒掉或吸掉含丝裂霉素C的培养基，磷酸盐缓冲液清洗5遍，尽量除去丝裂霉素C，加入2 mL 0.05%的胰蛋白酶消化细胞，镜下观察，当细胞间出现裂隙，细胞变圆时（约2～4 min），立即加入等量的全培养液终止消化，并用吸管反复吹打，使之成为单细胞悬液，在细胞计数板中央放置专用的盖玻片，用玻璃虹吸管吸取细胞，让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液，至盖玻片下被液体充满为止。

Making inventory is a process which tracks down inventory, storage capacity, working gas volume and cushion gas volume in multicycle operation of underground gas storage. This process is a key step to study the UGS operation rule, analyze the gas loss, improve the operating efficiency, and reduce the cost.

地下储气库多周期运行盘库过程，是对气库库存量、库容量、工作气量以及气垫气量的全过程实施跟踪的过程，是研究气库运行规律、漏失分析以及进一步提高气库运行效率、降低运行成本的关键环节。

According to the character of condensate gas and the injected dry gas inside the UGS based on condensate gas reservoirs, the paper puts forward a mathematical model of multicycle working inventory for constant volume and weak waterbearing condensate gas reservoir by the mixed fluid density calculation method with molar volume balance and the gas equation of state.

为此，针对凝析气藏型地下储气库库内凝析气与注入干气混气的特点，从库存量的定义入手，利用摩尔体积权衡混合流体密度计算方法和气体状态方程，提出了定容和弱边底水凝析气藏型地下储气库多周期运行系列盘库计算的数学模型。

We hypothesized that infusion of 0.9% saline in conscious, chronically instrumented sheep with hyperdynamic bacteremic sepsis would be associated with less plasma volume expansion and greater interstitial fluid volume expansion than in conscious, nonseptic sheep.

我们常假设：给患因感染高活力细菌而导致脓毒症并长期被仪器监测的清醒的羊输注0.9％的生理盐水会比给清醒的没有患脓毒症的羊输注0.9％的生理盐水血容量的扩张少而组织间液的扩张大。

Produce trimellitic acid octyl three main export products and domestic production for enterprises supporting materials, production, domestic sales volume the first in the industry; trusted by the user as the quality of products, octyl sebacate export volume has .

所生产的偏苯三酸三辛酯主要与国内生产出口产品企业合作配套作原料，产量、销量居国内同行业之首；被用户誉为质量信得过产品，癸二酸二辛酯有批量出口。

The bezoar sterilization piece, the antibacterial sterilization piece, the bone fracture bruise capsule in the similar variety the national sales volume first, the wood uses up the capsule in osteoarthropathy medication the national sales volume first.

牛黄消炎片、抗菌消炎片、骨折挫伤胶囊在同类品种中全国销量第一，木竭胶囊在骨关节病用药中全国销量第一。

However, during borehole grouting pile construction of the present, round section piles are used universally. Generally speaking, the boreholes are drilled by a large diameter round piling bit. Because of the restriction of factors of the mud pumps, and drilling equipments, and drilling technique, it would result in such problems as fol lowing: a bigger cutting area and overlarge cuttings volume, and more hole bottom sediments, difficult cuttings discharging, and serverely repeat cuttings breaking, and using more concret grouting amount, and lower drilling speed, faster bit wearing, and longer working period, bigger material and labor ex haust, and higher engineering cost, unreasonable ratio of loading capacity to volume of pile foundation, and lower comprehensive economic benefits, and so forth.

然而，钻孔灌注桩施工中，目前最为普遍采用的是圆形断面桩基，一般采用大口径圆形基桩工程钻头，由于国内生产的泥浆泵、钻具及钻进方法等因素的限制，从而使钻孔破岩面积和体积过大，造成孔底沉渣过多，排渣困难且排浆量大，重复破碎问题突出，混凝土灌注量大，直接反映为钻进效率低、钻头磨损快、施工工期长、材料能耗和劳动消耗大、工程成本较高、桩基荷载能力与体积比不合理、综合经济效益不理想等，这些问题严重影响和制约着钻孔灌注桩施工技术的发展和进步。

These are places without aristocratic baggage; egalitarian places open to talent, self-improving, engaged in learning and innovation through networks that were at once competitive and cooperative.

这些地方没有贵族遗风作祟，而且对于那些有天分的人是开放的平等之地，这些人善于通过那些曾经很具有竞争力与合作精神的关系网进行自我提高以及学习创新。

Christine: You don't want to see me?

你不想见我？