查询词典 virus disease

There are three sections in this dissertation: 1 quantification of gene expression in chicken embryo fibroblasts and virus load in bursal Fabricius infected with infectious bursal disease virus, the recombination of infectious laryngotracheitis virus and pathotyping of Newcastle disease virus.

本论文包括三个部分：1传染性法氏囊病病毒对鸡成纤维细胞某些基因表达的影响和病毒在法氏囊与粪便中的定量研究；2传染性喉气管炎病毒的重组；3新城疫病毒的分型研究。

The protocol for the detections of all the AIV isolates, the H5-subtype and the optimization of reactions were studied with a H5-subtype reference isolate BSG1. A 229bp-band of AIV specific and a 380bp-band of H5 subtype specific were amplified individually with the designed primers of AIV specific and H5-subtype specific respectively by the developed RT-PCR technique from isolate BSG1. A double-PCR was also developed which can detect all the AIVs and identify the H5-subtype AIVs. The results of specificity experiment showed that there was no specific band could be amplified with the samples of Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, egg drop syndrome virus and infectious bursal disease virus.

结果应用通用引物可从BSG1毒株扩增到与预计大小相符的229bp AIV特异性片段，应用H5亚型鉴定引物则可从BSG1毒株扩增到与预计大小相符的380bp H5亚型特异性片段；在此基础上建立了可检测所有AIV毒株以及可同时进行H5亚型毒株鉴定的二重RT-PCR方法；检测方法的特异性试验结果表明，该方法对新城疫病毒、传染性支气管炎病毒、传染性喉气管炎病毒、产蛋下降综合症、传染性法氏囊病毒无交叉反应；检测方法的敏感性试验结果表明，通用引物扩增、H5亚型特异性引物扩增以及二重PCR扩增反应的组织样品总RNA的最低检出量分别为0.0217μg、0.0217μg和2.17μg；对20份临床样品检测中，有10份为AIV阳性，其中有5份为H5亚型病毒阳性。

this article be main to used for the chicken non- typical model epidemic disease in Hsin-cheng, the contagion method surname bag, geniality flue, contagion bronchitis, contagion enterogastrtis, breed with breath system comprehensive disease, small virus, foot-and-mouth disease virus, false rabies;The kidney disease of the marine products animal fish disease, the issue of blood openings disease, crazy visit a disease, the terrapin issue of blood bowel way be bad dead, turtle mumps, grass carp bleed far-gone virus the Wen be febrile to prevent°from.

本品主要用于鸡非典型新城疫，传染性法氏囊，温和性流行性感冒，传染性支气管炎，传染性胃肠炎，繁殖与呼吸系统综合症，细小病毒，口蹄疫病毒，伪狂犬病；水产动物鱼病肾脏病，出血性开口病，狂游病，鳖出血性肠道坏死，甲鱼腮腺炎，草鱼出血病等病毒性瘟热病的防止。

A new wheat virus disease was reported in Jiangsu province, China. Wheat plants infected showed chlorotic or yellow stripes which was continuous or discontinuous on the leaves, the diseased new leaf became yellow or seared and the spike was unseeded.

为了确诊在江苏小麦上新发生的一种病毒病害，采用ELISA、RT-PCR和序列测定等方法对其病原进行了精确鉴定，并对此病的田间发生流行规律进行了调查分析。

There were no other reports on cytopathological research on virus disease of pumpkin in China until now.

对南瓜病毒病的细胞病理研究国内尚未见同类报道。

In clean diluent condition, the median lethal concentration (MLC50) of CIO2 against bacteria and yeast was 9.4 and 8.4 ppm, respectively. In dirty diluent condition, the MLC50 of CIO2 against bacteria and yeasts was 39.9 and 24.3 ppm, respectively. The virucidal activity of CIO2 against 100 median embryo lethal dose (100 ELD50) of Newcastle disease virus, avian influenza virus, infectious bronchitis virus and infectious bursal disease virus was 1.2, 1.2, 2.5 and 1.2 ppm, respectively.

发现CIO2在乾净稀释液中对细菌和酵母菌的中间致死浓度(MLC50)分别为9.4和8.4ppm，CIO2在污浊稀释液中对细菌和酵母菌的MLC50分别为39.9和24.3ppm，CIO2之杀灭新城病病毒、H6N1型家禽流行性感冒病毒、传染性支气管炎病毒和传染性华氏囊炎病毒的浓度分别为1.2、1.2、2.5和1.2ppm。

The company established safe breeding model after research on abalone breeding procedure especially on diet selection and breeding to improve survival rate obviously. It conducted research on common diseases hazarding abalone breeding, especially on spherical virus disease with most harm.

通过对鲍鱼育苗过程研究，尤其是饵料筛选与培育，建立了安全育苗模式，明显提高了育苗成活率；调查了危害鲍鱼养殖和育苗过程中常见的病害，对危害性最大的球状病毒病进行了深入的研究，并提出了有效的防治措施。

The infectivity of TMV recovered by dialysis from incubation with anthraquinone glycosides in vitro has been reduced in contrast to infectivity of TMV control, it seems that anthraquinone glycosides have direct viricidal effect on TMV. The anthraquinone glycosides may interfere recognization of TMV to host receptor when applied before TMV inoculation. The anthraquinone glycosides can inhibit multiplication of TMV in infected leaf discs but did not inhibit infectivity of TMV-RNA in vitro. The control of tobacco mosaic virus disease of N.

总蒽醌甙对TMV混合，对粒体形态无破坏作用，但经透析，其抑制作用仍较高，表明总蒽醌甙与TMV结合能力强不易解离或对粒体具有直接毒害作用；总蒽醌甙预先接种可能影响病毒对侵染位点的识别，对叶圆片中的病毒具有抑制复制的作用，但在体外混合不影响TMV-RNA的侵染活性，可能与总蒽醌甙干扰病毒的衣壳装配过程或影响复制酶的活性有关。

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

High resistance to tomato late blight, gray mold, virus disease, resistance to nematodes.

高抗番茄晚疫病、灰霉病、病毒病，耐线虫病。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。