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Methods Aimed at vesicular stomatitis virus, sindbis virus, human immunodeficiency virus, polioviruses, pseudorabies virus,encephalon-yocarditis virus; validate data of viral inactivated on pasteurization of albumin, human rabies immunoglobulin with pH 4, human immunoglobulin and human hepatitis B immunoglobulin for intravenous injection with pH 4/pasteurization,treatment on human fibrinogen and human coagulation factor Ⅷ with solvent/detergent and vapor heating at 100 ℃30 min were systemic regularized respectively.The mean and standard deviation also coefficient of variation for virus survival titer in different time were stated, dynamics curve of virus inactivation were made and analyzed.

系统性整理针对水疱性口炎病毒、黄热病毒、脊髓灰质炎病毒、伪狂犬病毒、脑心肌炎病毒、人类免疫缺陷病毒，人血白蛋白采用巴氏消毒法，狂犬病人免疫球蛋白采用低pH常温孵放法，静注人免疫球蛋白、静注人乙肝免疫球蛋白采用低pH/巴氏消毒法；人纤维蛋白原、人凝血因子Ⅷ采用有机溶剂/去污剂与干热法灭活病毒的验证资料，统计3批样品多个取样点的残余病毒滴度均值、标准差与变异系数，制作灭活病毒动力曲线图并进行分析。

This paper divided the mix type virus into the mail-virus and worm virus, and then analyzed thoroughly the essential characteristics and spreading means of these three kinds of different viruses. According to characteristic of these viruses, this thesis put out a project of "Intelligent Macro Code Scanning Technique" to macro-virus, a project of "Base of Command Code Scanning Technique" for mail virus and a project of "File Head Check-Up Method" for worm virus to protect file of PE type.

根据这些病毒的不同特征和传播手段等特点，对宏病毒提出了一种"智能宏代码扫描技术"的方案，对邮件病毒提出了一种"基于命令式的代码扫描技术"的解决方案，对蠕虫病毒的解决则是以保护PE格式文件为重点提出了一种"文件头检测法"的解决方案。

Some key techniques related to the close and continuous process were investigated by the application of H9N2 avian influenza virus with Vero cells, such as the susceptibility of cell to influenza virus, virus production with cell microcarrier culture method, cell bead-to-bead transfer, virus production through bead-to-bead transfer, cell culture and virus production with serum free medium, metabolism analysis, and repetitiously intermittent bead-to-bead transfer of cell for virus production to simulate the close and continuous process.

通过使用Vero细胞增殖禽流感病毒H9N2，本文针对封闭连续工艺过程的一些关键技术开展研究，包括细胞对流感病毒敏感性分析、细胞微载体培养生产病毒工艺、细胞珠到珠转移、转移后细胞对病毒增殖敏感性验证、细胞无血清培养生产流感病毒、代谢分析、可模拟连续操作的多次间歇式珠到珠转移培养细胞生产流感病毒等方面。

Porcine hemoglobin polymer is made in imitation as the substtitute for blood product. Pseudorabies virus is chosen as the enveloped DNA model virus and the Porcine Parvovirus is chosen as the nonenveloped DNA model virus. Complete DNA sequences of these two kinds of virus are analyzed and specific fragments are selected as examinable targets. Establish a new method based on real time PCR combined with cell infection assay to evaluate virus clearance efficiency in blood product.A new method is established to remove the virus from blood product.

本研究以戊二醛聚合猪血红蛋白模拟血液制品，选取PRV、PPV分别作为血液制品中有包膜DNA指示病毒和无包膜DNA指示病毒，针对特定两种指示病毒PRV、PPV，进行了生物信息学分析，选取特定片段作为扩增靶点进行检测，建立了一种基于实时荧光定量PCR技术的检测方法，联合病毒的细胞感染力实验，用以评价血液制品中指示病毒的灭活去除效率。

The four experimental virus strains and the three collate virus strains were divided into two groups, and belonged two evolutionary branches. Although ADV-DL1, DL2 and DL3 were all belonged to the Da Lian separated strains, they had the ulterior genetic relationship. The nuclear sequences of the obtained experimental ADV virus strains, ADV virus collate virus strains and typify virus strains of other four Parvovirus generic were cladogram analyzed with the Clustal W method of software DNA Star.

结果表明，ADV-Utah与四个实验毒株的序列同源性较高，同源率为92.9-93.4％，ADV-G、ADV-SL3与实验毒株的同源性较低；四个实验毒株和三个参考毒株被分别划归为两个组，分属于两个进化分支；实验毒株中ADV-DL1与DL2和DL3虽然同为大连分离株，却表现出较远的亲缘关系。

Results The highest positive rates were found in respiratory syncytial virus (RSV,30.5%) and adenovirus (AdV,27.4%),and the other findings were as following: Coxsackie virus (CV,11.3%), EB virus (EBV,6.5%),echovirus (ECHOV,4.8%),parainfluenza virus (PIV,1.6%), influenza virus B (IVB,1.6%) and influenza virus A (IVA,0%).

结果 呼吸道合胞病毒、腺病毒阳性率最高，分别为30.5%、27.4%，其次为柯萨奇病毒、EB病毒，分别为11.3%、6.5%，埃可病毒为4.8%，副流感病毒及流感病毒B均为1.6%，流感病毒A为0%。

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

Partial DNA-A sequence comparison with other geminiviruses showed that G7 were infected with 5 begomoviruses, these viruses were Papaya leaf curl China virus, Ageratum yellow vein china virus, Tomato leaf curl China virus, Euphorbia leaf curl virus and Tobacco leaf curl Yunnan virus.

得到部分G7的DNA一A序列，经NCBI检索和序列比较分析发现了5种菜豆金色叶病毒属病毒的部分DNA序列，分别是中国番木瓜曲叶病毒、中国胜红蓟黄脉病毒、中国番茄曲叶病毒、一品红曲叶病毒、云南烟草曲叶病毒。

Annual the tall hair that Spring Festival long holiday is virus period, during virus uses the Spring Festival, people each other sends a blessing, scanty to virus at the space that be on guard, send the link that contains virus or document through QQ or MSN, and once the user is clicked,the file is met toxic, toxic hind virus can send same instant message to all and online good friend, create instant news report user interlink infection.

每年春节长假都是病毒的高发期，病毒利用春节期间人们互发祝福，对病毒疏于防范的空隙，通过QQ或MSN发送带有病毒的链接或文件，而一旦用户点击此类链接或文件就会中毒，中毒后病毒会向所有在线好友发送同样的即时消息，造成即时通讯用户连环感染。

Biology technique is based on appearance symptom on sweet potato or Ipomoea Setosa after they are infected to identify virus. But it need long period and is apt to be limited by season. Based on coat protein of virus, serology detects virus during peculiar immunology reaction of antibody and antigen. But it can't detect virus without CP and preparing antibody need more time. The molecular biology method is based on nucleic acid to detect virus. This technique has high sensitivity, strong veracity, wide suitability etc.

生物学方法是依据病毒侵染后在甘薯或指示植物上的外观症状进行识别，但检测速度慢，易受季节限制；血清学技术是利用病毒外壳蛋白的抗原性，据特异性的抗体抗原免疫学反应检测病毒存在，但制备抗血清需时间长，对含量少和无蛋白外壳的病毒无法检测；分子生物学方法是以核酸为基础，通过检测病毒核酸来证实病毒的存在，该技术具有灵敏度高、特异性强、适应范围广等特点。

Panorama Many people are dancing in a large sitting room.

在一个大客厅里，许多人在跳舞。

We fully understand how a microwave oven cooks food, it is first necessary to talk ...

我们充分理解一个微波炉煮的食物，这是首先要谈。。。