查询词典 vector analysis

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

Using the total RNA of merozoites of Eimeria acervulina isolated from Guangdong Pro- vince as template,a partial segment of 3-1E gene was amplified by RT-PCR. The gene fragment 682 bp inlength was cloned into pGEM-T Easy vector,and the recombinant plasmid was identified by PCR,restric-tion enzyme analysis and sequencing. The homology analysis revealed that the nucleotide homology be-tween the 3-1E gene of the E.

根据GenBank中登录的堆型艾美球虫3-1E基因序列，设计了3条引物，以广东株堆型艾美球虫裂殖子总RNA为模板，利用反转录-聚合酶链反应扩增获得了3-1E基因部分片段，将这一片段克隆至pGEM-T Easy载体中，经PCR、限制性内切酶分析和克隆片段的序列测定、比较，证实了克隆片段的可靠性。

Mainly, the article divides into six contents: system development background and method of exploitation, demand analysis, system analysis, system design, system implementation and conclusion. In demand analysis, has established first with the example model, and comes the detailed description through the active chart to use the example. Through the sequence chart and the VOPC chart, I completed the definition of boundary, control and data entity of the fore-platform in system analysis. The system design mainly has carried on the detailed design to kind of and the database.

在需求分析中，先建立了用例模型，通过文字和活动图来详细的描述用例，确定了前台子系统的各个功能模块；系统分析中，使用序列图和VOPC图完成了对系统运作过程中涉及的界面、控制、数据实体的定义；系统设计主要主要对类及数据库进行了详细的设计；系统实施中，对前台子系统的物理部署、实施环境进行描述，包括系统的主要界面和部分代码。

According to different types of ambiguity, five means is used to resolve the ambiguity, that is: analytic hierarchy analysis, transformation, analysis of semantic feature, semantic orientation analysis and contextual analysis.

根据不同的歧义类型，用五种方法对歧义进行化解：层次分析法，变换分析法，语义特征分析法，语义指向分析法和语境分析法。

NMath Stats contains a data table class with functions for computing descriptive statistics, such as mean, variance, standard deviation, percentile, median, quartiles, geometric mean, harmonic mean, RMS, kurtosis, skewness, and many more; PDF, CDF, inverse CDF, and random variable moments for a variety of probability distributions, including normal, Poisson, chi-square, gamma, beta, Student's t, F, binomial, and negative binomial; Combinatorial functions, such as factorial, log factorial, binomial coefficient, and log binomial; Multiple linear regression; Basic hypothesis tests, such as z-test, t-test, and F-test, with calculation of p-values, critical values, and confidence intervals; One-way and two-way analysis of variance and analysis of variance with repeated measures; Multivariate statistical analyses, including principal component analysis and hierarchical cluster analysis.

nmath统计包含一个数据表的阶层与职能计算描述性统计，如平均，方差，标准差，百分位，中位数， 25 ％，几何平均数，调和的意思是，有效值，峭度，偏度，还有更多的； PDF格式，民防部队，逆民防部队，并随机变量矩的各种概率分布，包括正常，泊松，卡方检定，伽玛，测试版，学生的吨，男，二项式，并负二项分布；组合的功能，例如阶乘，日志阶乘，二项式系数，并登入二项式；多元线性回归；基本假设测试，如的Z测试， t检验， F检验，计算p值，临界值，和置信区间；之一，双向和双方法方差分析和方差分析与反复的措施；多元统计分析，包括主成分分析和聚类分析。

At the time of the risk analysis, analyze break-even analysis and sensitivity analysis, and further analyze the sensitivity analysis of the sales revenue respectively and total investment.

在进行风险分析时，分别进行了盈亏平衡分析和敏感性分析，在敏感性分析中分别对销售收入和总投资进行了分析。

①Location of WD gene in Ch inese: Using pairwise linkage analysis and multipoint linkage analysis method, w e constructed a genetic map of DNA markers within D13q14.2-3 which refined the location of WD gene by restriction fragment length polymorphism and microsatellite polymorphism analysis;②Screen for mutations of WD gene in Chinese people: we detected the structure of 21 exons of WD ge ne in 45 patients from 39 pedigrees by PCR-SSCP(Single strand conformation poly morphism) and PCR-DNA sequencing technology, found a new mutation in exon 5 and nuclcotide sequence analysis showed it is a T insertion. We also conformed the Arg778Leu in exon 8, the highest frequence mutation point in Chinese people, wit h mutation rate 22.8%in total;③Carrier detection and presymptomatic diagnosi s of WD: Based on DNA recombination technology, we peformed successfully the gen e diagnosis in all individuals of 79 families with WD and built up a helpful spe cific enzyme cut method (PCR-Msp1) to detect the carrier and presympomatic patients in Chinese pe ople with WD.

①WD的基因定位研究：通过RFLP及微卫星多态性分析，应用两位点及多位点连锁软件，建立了中国人WD基因在D13q14.2-3区域的精细遗传连锁图谱，从而首次对中国人WD基因进行了精确定位；②WD基因突变研究：应用PCR-SSCP及DNA测序技术，对39个家系45名WD患者进行该致病基因的21个外显子突变筛选，发现WD基因5号外显子存在新的T插入突变，并证实中国人WD基因的突变热点为8号外显子，突变形式为Arg778Leu，其频率为22.8%；③WD的症状前诊断和杂合子检出：应用DNA重组技术对79个家系进行基因诊断，成功地进行了WD的症状前诊断和杂合子检出，并建立了WD的基因筛选的PCR-Msp 1酶切方法。

This course will mainly discuss on time-domain analysis and frequency-domain analysis of determined signal, description and characteristic of linear time-invariant system, as well as time-domain analysis and transformation domain analysis of signal via linear time-invariant system.

本课程主要讨论确定性信号的时域和频域分析，线性时不变系统的描述与特性，以及信号通过线性时不变系统的时域分析与变换域分析。

Based on the analysis of different methods for appraising the port resource economic effect, the data envelopment analysis and modified data envelopment analysis models are introduced to make a comprehensive appraisal of economic effects of 12 Chinese container terminal companies, and the comparative analysis of the relative effective values obtained by DEA and MDEA models is made.

在分析港口资源经济效益评价方法的基础上，引入DEA及MDEA模型，对我国12家集装箱码头公司的经济效益进行了综合评价，并对它们分别得出的相对有效值进行比较分析。

In this research, the SOLO (Structure of the Observed Learning Outcome) Taxonomy presented by Australian scholars Biggs and Collis (1982) was adopted, and students studying the fifth and sixth grades in Taipei area were chosen as research targets.This study was primarily implemented with a quantitative analysis and supported by a qualitative analysis. In order to carry out the research, a questionnaire had been designed with criteria suggested by Jones et al.(2000), including "describing data","organizing and reducing data","representing data" and "analyzing and interpreting data." This questionnaire consisted of open-ended questions, and after analyzed with qualitative methods, the responses were classified and scored based on standards set in SOLO Taxonomy. Then the quantitative method "Partial Credit Analysis" from Rasch model was adopted in examining the reliability, validity and consistency of outcomes from the qualitative analysis.

提出的统计思维架构－「描述资料」、「组织及简化资料」、「呈现资料」、「分析资料」作为评量试题的向度，题型为开放性问答题，在区分学童的答题表现方面，采用质的分析，参照SOLO（Structure of the Observed Learning Outcome）分类法的分类标准，将学童不同的答题反应进行分类，给予不同的分数，最后再采用Rasch model中的部份给分模式进行量化分析，以量化数据佐证质性分析的信度与效度，以及不同给分的吻合度。

"The operators will suffer a temporary decrease in the number of consumers and short message services as people may not like having to provide their ID cards for a phone number," Xu Junqi, vice-dean with the Policy-Making Institute of the Telecommunications Research Centre under MII, said yesterday.

信息产业部下属的通信决策研究院副主任徐俊其昨天说：&实行手机实名制后，人们可能不太情愿用身份证注册手机号，所以通信运营商会暂时面临用户减少和短信用户减少的问题。&

I don't believe in make-believe!

我不相信这些虚构的故事。