查询词典 vector addition

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

The total proteins from TGIF transfectant and vector control cells were separated by 2D electrophorosis. 760±41 and 680±38 spots were detected in TGIF transfectant and vector control cells respectively. Locus repeat analysis of protein spots showed that the mean deviation in IEF direction in TGIF transfectant and vector control cells were 0.864±0.123 mm and 0.843±0.115 mm respectively whereas the mean deviation in SDS-PAGE direction in these two cells were 0.812±0.109 mm and 1. 125±0.123 mm respectively. So the well-resolved and reproducible 2DE patterns from TGIF transfectant and vector control cells were established.

通过双向凝胶电泳分别分离TGIF转染细胞和PcDNA3.1空白质粒转染细胞的总蛋白质，测得两组细胞的蛋白质斑点数分别为760±41和680±38个；位置重复性分析表明TGIF转染细胞在等电聚焦方向上的平均偏差为0.864±0.123mm，在垂直板SDS-PAGE电泳方向上的平均位置偏差为0.812±0.109mm；PcDNA3.1空白质粒转染细胞在IEF方向上的平均偏差为0.843±0.115mm，在垂直板SDS-PAGE电泳方向上的平均位置偏差为1.125±0.123mm。

The System inset remote sense vector data and GIS vector data in drainage area into client programs programmed, realizing the application of remote sense vector data and GIS vector data in water resources management of Xinjiang Ma-na-si River irrigation area, and realizing the integrated management of the texts and maps of the water resources survey data, and spatial analysis and map edit functions of GIS as well. The system, which breaks away the platform of MapInfo, can run independently.

该系统将流域中的遥感栅格数据与GIS矢量数据集成嵌入到所编制的客户程序中，实现遥感数据和GIS数据在新疆玛纳斯河流域水资源管理中的应用，和水资源调查数据图文一体化的管理，同时具备了GIS的空间分析和图形编辑等功能，该系统能脱离Mapinfo软件平台独立运行。

Is a collection of some of the attributes of all things; vector is also value addition to the amount of direction at the same time; vector space, also known as linear space, is composed of many components of the vector and in line with the specific rules of operation of the collection.

集合是具有某种属性的事物的全体；向量是除了具有数值还同时具有方向的量；向量空间也叫线性空间，是由许多向量组成的并且符合某些特定运算的规则的集合。

If physical quantity is vector quantity it must obey the addition rule of vector quantity, that is to say, when two vector quantities is added, its result is not influenced by adding order.

物理量又分为标量、矢量和张量，若物理量是矢量，它应服从矢量的加法法则，即两个矢量相加时，相加次序不影响它的结果，由A→+B→=B→+A→所得到的最终结果相同。

The results show that the grain size is refined by the addition of Nd or Dy, especially combined addition is better than single addition. With the addition of 0.2 % Nd and 0.6% Dy, the grain size is reduced from 150 μm to 60 μm. Combined addition with two rare earths elements makes the tensile strength, yield strength and elongation of ZK10 alloy increase to 191.0 MPa, 69.0 MPa, and 16.6%, increasing by 89%, 43%, 232% respectively, compared with ZK10 alloy without RE element.

结果表明：ZK10镁合金中分别加入Nd和Dy均能细化晶粒，且Nd和Dy同时加入的效果优于单一加入的效果，当同时加入质量分数为0.2% Nd和0.6% Dy时，合金的平均晶粒尺寸由原来的150 μm细化至60 μm，使ZK10合金的抗拉强度和屈服强度分别提高至191.0 MPa和69.0 MPa，伸长率达到16.6%，与未加入稀土的ZK10合金相比分别提高89%，43%和232%。

To obtain a sparseness of support vector for least squares support vector regression, an algorithm called vector base learning is proposed in this paper.

使最小二乘支持向量机的解具有稀疏性，本文提出一种稀疏解算法——矢量基学习。

First, commonly used methods of flight segment volume forecasting are systematically elaborated, Then theory basis of support vector machine and its principle are introduced, support vector machine model is inferred, the support vector machines algorithm is studied.

首先，系统地介绍了航空运量预测中常用的预测方法，并对几种常用方法的特点进行了分析比较。

In the fourth chapter, firstly it introduces the Boolean functions algebraic expressions of the 2-value clock-controlled stop-and-go generator and Gunther generator. It reveals the balanced property of the two kinds of Boolean functions, and studies the Walsh cycle spectrum and the autocorrelation function. It also obtains the coincidence rate of their output sequences with affine sum of some bits of input sequences, and analyzes their ability of resisting the best affine approximation cryptanalysis and differential cryptanalysis. Secondly, we properly present a new definition of the Best Affine Approximation, namely BAA on the Boolean vector functions, followed by the spectral characteristic of such defined BAA attacks through using the decomposition formula of the union distribution for random variables. A lower bound of such BAA attacks is proposed. Finally, we also study the spectral characteristic of the second kind of nonlinearity of Boolean vector functions, followed by a higher bound of such nonlinearity. Furthermore, the limited relationship between the second kind of nonlinearity of Boolean vector functions and the linear structure of the linear combination of every component is analyzed.

在第四章中，首先给出了2值密钥流"停走生成器"和"衮特生成器"中实际存在的布尔函数的代数表示，揭示了这两类布尔函数的平衡性，随后研究了它们的Walsh循环谱和自相关函数等，得到了它们的输出序列与输入序列中的某些bit的仿射项的符合率，分析了它们抵抗最佳仿射逼近攻击和差分攻击的能力；其次，我们合理地给出了布尔向量函数最佳仿射逼近的新定义，利用布尔随机变量联合分布的分解式考察了相应的谱特征，并给出了布尔向量函数与其最佳仿射逼近的符合率的一个下界；最后，我们还考察了布尔向量函数第二类非线性度的谱特征，给出了布尔向量函数第二类非线性度的一个上界，并揭示了布尔向量函数第二类非线性度与其各个分量的线性和的线性结构之间存在的制约关系。

Using the theories of probability, algebra and spectral theory comprehensively, we investigate some related characteristics of logic functions in cryptography: Firstly, we introduce m order generalized s - correlation immunity of Boolean vector functions and prove that the higher order generalized ε- correlation immunity can guarantee the lower order generalized ε- correlation immunity. Then by applying decomposition formula of joint distribution of Boolean random vectors, we give a spectrum criterion of m order generalized e - correlation immunity of Boolean vector functions. Furthermore, we show that the algebraic degree of m order generalized e - correlation immune Boolean vector functions is not restricted by the correlation immune orders.

本文主要运用概率论的思想和方法，并结合代数学和频谱理论的相关知识，对密码学中逻辑函数的有关性质进行了研究，主要包括以下三个方面的内容：首先，对布尔向量函数的相关免疫性进行了拓展，给出了k维布尔向量函数m阶广义ε-相关免疫的概念，证明了布尔向量函数的高阶广义ε-相关免疫性蕴含低阶广义ε-相关免疫性，并根据布尔随机向量联合分布分解式得到了布尔向量函数m阶广义ε-相关免疫的一个谱判别条件，还说明了m阶广义ε-相关免疫布尔向量函数的代数次数不受相关免疫阶数的制约。

That snake Alaric named Archbishop...

庇护。。。那个毒如蛇蝎的Alaric被命名为大教主。。。

You have done lots of magical things to be amazed at.

你做了很多神奇的事物感到惊讶。