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But it demands the higher computational accuracy. Time-dependent LU-SGS method and close coupled full implicit finite volume scheme are used. The AUSMPW+ based on the three-order MUSCL difference scheme is used for the inviscid operator, and the maximum eigenvalue splitting method is used to convert matrix operation to algebraic operation in order to shorten the computing time.

运用时间相关的LU-SGS方法，采用强耦合的全隐式有限体积进行离散，其中无粘对流项采用三阶MUSCL型差分的AUSMPW+格式，并应用最大特征值分裂，将矩阵运算转化为代数运算，大大缩短了计算时间。

Giemsa staining was used to observe the growth of C.andersoni,and transmission electron microscope was used to observe the developmental stages of C.andersoni.MTT assay was used to evaluate the toxicity of nitazoxanid and ginkgoic acids to HCT-8 cells.C.andersoni parasites growing in HCT-8 cells were treated with different concentrations of NTZ and GAs,and the copies of COWP gene were determined by Real-time PCR.

安氏隐孢子虫卵囊感染人结肠癌(HCT-8)细胞，Real-time PCR技术测定RPMI-1640培养液中分别加入不同浓度胎牛血清(fetal calf serum，FCS)、葡萄糖(glucose，GLU)、维生素C(ascorbic acid，ASC)、叶酸(folic acid，FOLIC)、泛酸钙(calcium pantothenate，CAPAN)和胰岛素(insulin，INSULIN)后虫体的增殖效果。

The author collected data by using comprehensive assessment and pencil-talking from January 8 to February 15, 2007 and identified chronic pain, impaired tissue integrity, and death anxiety of the case problems. For chronic pain, sleeping pillow with cold water, massage lymph and acupoints of head and neck, music and aroma therapy were used to release symptoms of the case pain. For impaired tissue integrity, during wound care, normal saline and furacin gauzes were used to wet-packing to release wound bleeding, and tree oil aroma put into the covering gauze to release the smelling, and used duoderm and low-allergic tapes to protect the skin around the wound.

笔者於2007年1月8日至2月15日期间藉由整体性护理评估及笔谈方式收集资料，确立个案有慢性疼痛、组织完整性受损、死亡焦虑等问题；针对慢性疼痛问题，采睡凉水枕、头颈部淋巴及穴位按摩、音乐及精油辅助疗法等措施来缓解个案的疼痛症状；针对组织完整性受损问题，於伤口护理时，选择生理食盐水湿纱和furacin纱布进行伤口湿敷，以缓解伤口出血，并於外层敷料加入茶树精油缓解伤口恶臭，同时运用人工皮及低过敏胶带来保护伤口周围皮肤。

Although many researchers have studied the synthesis of allyl phenyl ether by phase-transfer catalysis, no studies have used it in membrane reactor up to now. The purpose of this paper is to combine the studies of membrane reactor and phase-transfer catalysis. The membrane was used to stabilize the aqueous/organic interface at a fixed position, also the quaternary ammonium ion in the membrane was used as the catalyst to partake in the reaction system directly, and so it can also separate the product and reactants at the same time. A theoretical model is also presented.

中文摘要对於利用相间转移触媒催化技术来合成丙烯基苯基醚的反应，已有不少专家学者研究过，而至今还无在薄膜反应器中进行此反应之研究，本研究则是要结合薄膜反应器与相间转移触媒两者之特性，固定水油两相之界面，并且利用薄膜上的四级铵离子为触媒，直接在反应系统中参与反应，并同时分离产物及反应物，进而推导出相关之理论模型。

METHODS: Switch mechanism at 5′end of mRNA template technology, SMART technology, was used to construct the libraries. The purified Poly+RNA was used as template, Powerscript reverse transcriptase was used to transcribe and 5′oligo as a short extended template was added to the 5′ end of mRNA to enrich the fulllength cDNAs.

运用mRNA 5′末端的模板转换方法，即SMART技术，以纯化的Poly+RNA为模板，用Powerscript逆转录酶进行转录，并在mRNA 的5′末端添加一段5′ oligo作为延伸后的模板，从而富集全长 cDNA。

METHODS: Switch mechanism at 5′end of mRNA template technology, SMART technology, was used to construct the libraries. purified Poly+RNA was used as template, Powerscript reverse transcriptase was used to transcribe and 5′oligo as a short extended template was added to the 5′ end of mRNA to enrich the fulllength cDNAs.

运用mRNA 5′末端的模板转换方法，即SMART技术，以纯化的Poly+RNA为模板，用Powerscript逆转录酶进行转录，并在mRNA 的5′末端添加一段5′ oligo作为延伸后的模板，从而富集全长 cDNA。

METHODS摘要: Switch mechanism at 5′end of mRNA template technology, SMART technology, was used to construct the libraries. The purified Poly+RNA was used as template, Powerscript reverse transcriptase was used to transcribe and 5′oligo as a short extended template was added to the 5′ end of mRNA to enrich the fulllength cDNAs.

方法摘要：运用mRNA 5′末端的模板转换方法，即SMART技术，以纯化的Poly+RNA为模板，用Powerscript逆转录酶进行转录，并在mRNA 的5′末端添加一段5′ oligo作为延伸后的模板，从而富集全长 cDNA。

Methods: One-step trizol had used to get the total RNA of the four animal brain, then PEGS000 to purify the small RNA from the total RNA, after the ligation ofpoly on the 3\' and the adaptor on the 5\', the oligo primer will be used to reverse translation the small RNA, and then used the specific primers to amplification the small RNA sequences. After the gel purition recovery, the small RNA will be ligated to the T-vector, and be transformed to the TOP 10 E. coli, at last, we picked up the masc. clones for sequencing.

通过Trizol一步法提取四种动物脑中的总RNA，接着用PEG纯化其中的小分子RNA，再经过poly连尾反应在小RNA的3'端连接上一段poly，然后在5'端连上adaptor，用Oligoprimer将连接好的小RNA进行反转录，用根据adaptor和Oligoprimer设计的引物来扩增小RNA，扩增后的小RNA进行胶回收后连接到T载体上，转化TOP10大肠杆菌后挑取阳性克隆进行测序，得到的序列再经过生物信息学的分析后确定是否为miRNA。

Chorus oh, you never turned around to see the frowns on the jumpers and the clowns when they did their tricks for you you never understood that it ain't no good you shouldn't let other people get your kicks for you you used to ride on your chrome horse with your diplomat who carried on his shoulder a siamese cat ain't it hard when you discovered that he really wasn't where it's at after he took from you everything he could steal princess on a steeple and all the pretty people they're all drinking, thinking that they've got it made exchanging all precious gifts you better take your diamond ring, you better pawn it babe you used to be so amused at napoleon in rags and the language that he used go to him he calls you, you can't refuse when you ain't got nothing, you got nothing to loose you're invisible now, you've got not secret to conceal Like A Rolling Stone

没了回家的方向、也没人认识像个流浪汉高傲的王子，和所有那些漂亮的人们他们都喝着酒，琢磨着怎么能淘换到更值钱的玩意儿但是你呢，你得把你的钻戒举起来，把它当了人都有倒霉的时候，看着戏里走背字儿的拿破仑你不是曾经还挺得意吗？现在轮到你了，拿破仑在招呼你了，你无路可退了当你一无所有的时候，你不用担心会失去什么了现在你已经透明了，已经没啥可隐瞒的了孤身一人的滋味你觉得怎么样？没了回家的方向、也没人认识像个流浪汉。

In order to disclosure the reason why the electrocatalysis activeness and selectivity droped after being used several hundred hours continuously, the x-ray diffraction, scanning electron microscopy and energy dispersive spectroscope have been used to characterize the surface condition variations of Monel metal. The results indicated: Monel metal belongs to the Cu-Ni alloy around the electrolysis experiment.And after being used for 600hr, the surface layer element composition and geometry distribution of the alloy changed, namely in the alloy the Cu element /which superficial free enthalpy was smaller,"concentrated" to the alloy surface,and formed one kind of marble structure microcrystallite.Thus the electrode surface becamed rough a

为了揭示蒙乃尔合金阴极在连续使用数百个小时后电催化活性剂选择性下降的原因，采用X射线衍射仪、电子扫描电镜和电子能谱分析仪对电解实验所用的蒙乃尔阴极材料在电解实验前后的表面状态进行了表征，结果表明：所使用的蒙乃尔合金阴极材料在电解实验前后均属于Cu-Ni合金，使用600hr后，由于表面层元素组成和几何排布发生了变化，即合金中表面自由焓较小的Cu元素向合金表面&富集&，形成一种大理石结构的微晶，表面粗糙、不均一，这是造成其对硝基苯电还原的催化活性及选择性随着使用时间延长而逐渐下降的主要原因，同时也为此类电极的修饰改进提供了方向。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。