查询词典 truncated sequence

These results suggested that the function of each function domain in HIV 1 LTR exhibited distinct characteristics on gag gene expression in vaccinia virus:(1) The function of intact LTR on gag gene expression varied with the upstream poxvirus promoter;(2) NR sequence neither decreased nor increased the Gag protein exprssion level;(3) Enhancer sequence might not be recognized by recombinant vaccinia virus expression system;(4) TAR sequence enhanced Gag protein expression effectively;(5) U5 region and its downstream non translated sequence had little effect on

免疫印迹和免疫酶试验检测均表明，6株重组病毒的Gag蛋白表达量因LTR不同而有明显差异，表明HIV1的LTR及其下游基因置于痘病毒启动子控制下，在痘苗病毒中表达时有下述特点：（1）不同的痘苗病毒启动子与全长LTR相互作用，对gag基因表达有显著不同的调控效果；（2）NR序列对Gag蛋白表达没有明显影响；（3）EN序列不能被重组痘苗病毒表达系统识别；（4）TAR序列可提高Gag蛋白的表达量；（5）U5区及下游非翻译序列不影响Gag蛋白的表达。

Sequence analysis revealed that nucleotides sequence of 28 kD protein gene varied among different isolates of wheat yellow mosaic virus.28 kD protein genes of the Luotian and Huangchuan isolates comprised 762 nucleotidesand were less 3 nt than others,located at the site of 326,327 and 336 nt,respectively.The different isolates shared homologies ranging from 92.1% to 96.9% in nucleotide sequence and 89.8% to 97.3% in amino acid sequence.

序列分析结果表明，小麦黄花叶病毒不同分离物的28kD蛋白基因的核苷酸序列存在一定的差异：湖北罗田和河南潢川分离物在第326，327和336位较四川雅安、江苏扬州及日本分离物缺少3个核苷酸，前两者核苷酸长度为762nt，后三者为765nt；不同分离物核苷酸序列同源性从92 1%到96 9%，相应的氨基酸序列同源性从89 8%到97 3%。

The characteristics of sequence development, source reservoir - seal assemblage and types of hydrocarbon reservoir for each sequence were discussed in detail. The dark colored shale of deep lacustrine facies in the transgressive system tract of SⅡ sequence deposited around the maximum flooding surface is the main source rock in this area. The shale of shallow lacustrine facies in the TST of SⅢ sequence around the MFS is the most important regional seal rock.

结果表明，该区古近系可划分为SⅠ，SⅡ，SⅢ和SⅣ4个层序，分别对应于房身泡组、沙三段、沙一段沙二段和东营组。SⅡ层序水进体系域最大湖泛期沉积的半深湖深湖相暗色泥岩是研究区的主力烃源岩系；SⅢ层序水进体系域最大湖泛面附近的浅湖相泥岩是最重要的区域盖层。

An image display apparatus includes a display having data holding function, a vertical drive circuit sequentially and selectively scanning matrix form display elements, and a horizontal drive circuit writing a voltage among binary voltage preliminarily assigned depending upon the digital data of the image signal. The horizontal drive circuit and the vertical drive circuit are operated for performing selective scan of respective display element for at least m times in one frame period. The vertical drive circuit is constituted of n number of sequence circuits and logic operation circuits for outputs of the sequence circuits, where n is smaller than m, a period from inputting to the sequence circuit to outputting from the final stage being less than or equal to half of one frame period, and at least one of the sequence circuits being used with selectively inputting a plurality of inputs.

提供了一种图象显示器，其具有显示部，在矩阵上排列的象素内保有数据保持的功能，根据保持的数据进行显示；垂直驱动电路，对构成显示部的矩阵状显示器以每行按顺序作选择性扫描；以及水平驱动电路，对于由垂直驱动电路选择的行显示器件，根据应显示的图象信号数字数据，从预先分配的二进制电压中写入电压，并利用水平、垂直驱动电路，与应显示的图象信号同步，在1帧期间至少m次对各显示象素进行选择性操作，以此进行多色调显示，垂直驱动电路由满足n＜m的n个顺序电路和其输出的逻辑运算电路组成，顺序电路的输入从最后级输出为止的期间为1帧期间的1/2以下，并且，n个顺序电路的至少一个的输入是切换多个输入系统而使用。

Truncated fragments of the amplified sequence were then subcloned into the 5＇of eukaryotic expression vectors with luciferase or chloramphenicol acetyltransferase gene as reporter genes.

将它们进行删切后在荧光酶和氯霉素乙酰基转移酶报告基因真核表达载体的上游构建了18个亚克隆并分别转染Jurkat细胞。

Methods:The whole mature protein coding sequence of three truncated dystrophin gene was amplified by RT-PCR method applied to human muscle cDNA. The fragment was inserted into prokaryotic expression vector pET28a plasmid.

以人肌肉组织cDNA为模板，采用RT-PCR扩增三个截短的肌营养不良蛋白cDNA，分别克隆入原核表达载体pET28a中，经限制性内切酶双酶切及DNA序列分析鉴定目的基因。

Using of matrix assisted laser desorption/ionization time of flight mass spectrometry coupled with carboxypeptidase Y digestion for the analysis of C terminal peptide ladder by direct determination of the mixture of truncated peptide, the sequence of C terminus can be read out simplely from the mass differences between adjacent peptide peaks on the mass spectrum.

用羧肽酶Y水解蛋白质和多肽，结合飞行时间质谱测定酶解后缩短肽的质量，根据相邻两肽峰的质量差别读出蛋白质和多肽的C端序列，在pmol水平下测定了人促肾上腺皮质激素片段以及人血管紧张肽片段，其中人促肾上腺皮质激素片段测到了C端 15个氨基酸残基的顺序

Methods Amplify the cDNA sequence encoding truncated HCV gene, with a part of carboxyl-terminus deleted, by PCR. Synthesize the mimic epitope at E2 region of HCV and seven T or Th cell epitope genes of NS3-NS5 respectively. Bind HCV core gene with a part of carboxyl-terminus deleted to the synthetic epitope gene by PCR, then clone into eukaryotic expression vector pcDNA3.1 and transiently transfect COS7 cells.

用PCR方法扩增核心区羧基端部分缺失的基因片段；分别合成HCV E2区模拟表位和NS3~NS5 7个T或Th细胞表位基因；PCR方法将羧基端部分缺失的HCV核心区基因与合成的表位基因串联，克隆入真核表达载体pcDNA3.1，并通过脂质体瞬时转染COS7细胞。

There are three features in the minibasins induced by gravitational sliding on the slope: the firstly, feature is surface of unconformity developed by onlap on minibasin margins or surface of unconformity truncated by mass transport-complex are sequence boundary in the study area; the secondly , accommodation of gravity flow is controlled by up lift rate and sedimentation rate , and styles of filling in the minibasins are dominated by the interrelationship between the two factors; and the third , there are two stacking patterns that consists of upward of turbidite fan-hemipelagic drape mud or mass-transport complexes-turbidite fan-hemipelagic drape mud.

认为在深水陆坡重力滑动作用所形成的微盆地内，层序边界表现为微盆地边缘上超不整合面或重力流对下伏地层的侵蚀不整合；逆冲构造隆升速率与沉积速率共同控制了重力流可容空间和沉积充填样式；沉积垂向演化以块状搬运复合体一浊积扇－深海披覆泥或浊积扇－深海披覆泥为特征。

Second, residues in the reactive site of the inhibitor were replaced by the consensus substrate recognition sequence of furin, namely, Arg at P_1, Lys at P_2, Arg at P_4 and Arg at P_6. In addition, the P_7 residue Asp was substituted with Ala to avoid possible electrostatic interference with furin inhibition. Finally, the extra N and C terminal residues beyond the doubly conjugated disulfide loops were further truncated. However, all resultant synthetic peptides were found to be temporary inhibitors of furin and kexin during a prolonged incubation, with the scissile peptide bond between P1 and P'1 cleaved to different extents by the enzymes.

高活性、低分子量的furin酶抑制剂有可能发展成为新型的抗细菌内毒素和抗病毒感染的药物，我们以绿豆胰蛋白酶抑制剂Lys片段长链22肽为模板，设计了一系列能抑制furin和kexin活性的突变体，采用如下三种方法逐步优化：一、去除Lys片段长链三对二硫键中的一对，用Ser替换Cys，减少可能存在的二硫键错误配对；二、抑制剂的活性中心根据furin底物的专一性逐步改变，即P1、P2、P4和P6位分别变为Arg、Lys、Arg和Arg，同时为了避免P7 Asp和P6 Arg之间可能存在的相互作用，将P7位Asp替换为Ala；三、将两对二硫键形成的双环的外面5个氨基酸残基去除。

The French are not suggesting a new EU treaty, but they have plenty of other wheezes.

法国人并不是在建议签署一个新的欧盟条约，但他们有许多其他的阴谋。

This letter was written by my brother.

这封信是我弟弟写的。