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MethodsThe D. bacteria - E. coli shuttle expression vector pZT17 was constructed based on plasmids of pUE30, pGBM5 and pKatCAT. Then pZT17 with lux+ from Photinus pyralis was used to transform into D. grandis and E.coli. The recombinant strains were induced separately.

以质粒pUE30、pGBM5及pKatCAT为基础，构建抗辐射菌属一大肠杆菌间的穿梭载体，将groEL启动子和荧光素酶基因lux+插入到构建的穿梭载体中得到穿梭表达载体，并将该载体转化大肠杆菌诱导荧光素酶基因的表达。

On the base of practical transformer substation main connection, electric components and system circuit model are first formed in the paper; analyze vacuum arc characteristic and medium resume are analyzed, then, according to vacuum chamber physical process of switching AC arc, resume process are divided into three stages: sheath development, neutral particles diffusion and static characteristic, with that, calculating model of medium resume strength is erected and applied in the computing of vacuum circuit breaker interrupting transform overvoltage; In the paper, Saber software is used to simulate a whole switching process; As follows, overvoltage characteristic of vacuum circuit breaker switching no load and load transformers, kinds of influence factors are analyzed in detail; At last, vacuum circuit breaker switching capacitance group overvoltage formative causes and electrode refining for lessening reignition are discuss.

本文针对中低压变电站的实际主接线建立各电气元件及系统的电路模型；在真空电弧的特点及间隙的介质恢复特性上，依据真空断路器灭弧室分断电弧的物理过程，将真空电弧熄灭后的恢复过程分为鞘层发展、中性粒子扩散和静态特性三个阶段，建立了相应的介质恢复强度的计算模型，应用到切除变压器操作过电压的计算中；采用Saber仿真软件模拟计算真空断路器切除变压器操作过电压；并分别对真空断路器切除空载及负载变压器操作过电压的特点与影响操作过电压的各种因素做了详细的分析；最后简要分析了真空断路器开断电容器组过电压的形成原因及为减少重燃的电极老炼措施。

On the base of practical transformer substation main connection,electriccomponents and system circuit model are first formed in the paper;analyze vacuum arccharacteristic and medium resume are analyzed,then,according to vacuum chamberphysical process of switching AC arc,resume process are divided into three stages:sheathdevelopment,neutral particles diffusion and static characteristic,with that,calculatingmodel of medium resume strength is erected and applied in the computing of vacuumcircuit breaker interrupting transform overvoltage;In the paper,Saber software is used tosimulate a whole switching process;As follows,overvoltage characteristic of vacuumcircuit breaker switching no load and load transformers,kinds of influence factors areanalyzed in detail;At last,vacuum circuit breaker switching capacitance groupovervoltage formative causes and electrode refining for lessening reignition are discuss.

本文针对中低压变电站的实际主接线建立各电气元件及系统的电路模型；在真空电弧的特点及间隙的介质恢复特性上，依据真空断路器灭弧室分断电弧的物理过程，将真空电弧熄灭后的恢复过程分为鞘层发展、中性粒子扩散和静态特性三个阶段，建立了相应的介质恢复强度的计算模型，应用到切除变压器作过电压的计算中；采用Saber仿真软件模拟计算真空断路器切除变压器作过电压；并分别对真空断路器切除空载及负载变压器作过电压的特点与影响作过电压的各种因素做了详细的分析；最后简要分析了真空断路器开断电容器组过电压的形成原因及为减少重燃的电极老炼措施。

On the base of practical transformer substation main connection,electriccomponents and system circuit model are first formed in the paper;analyze vacuum arccharacteristic and medium resume are analyzed,then,according to vacuum chamberphysical process of switching AC arc,resume process are divided into three stages:sheathdevelopment,neutral particles diffusion and static characteristic,with that,calculatingmodel of medium resume strength is erected and applied in the computing of vacuumcircuit breaker interrupting transform overvoltage;In the paper,Saber software is used tosimulate a whole switching process;As follows,overvoltage characteristic of vacuumcircuit breaker switching no load and load transformers,kinds of influence factors areanalyzed in detail;At last,vacuum circuit breaker switching capacitance groupovervoltage formative causes and electrode refining for lessening reignition are discuss.

本文针对中低压变电站的实际主接线建立各电气元件及系统的电路模型；在真空电弧的特点及间隙的介质恢复特性上，依据真空断路器灭弧室分断电弧的物理过程，将真空电弧熄灭后的恢复过程分为鞘层发展、中性粒子扩散和静态特性三个阶段，建立了相应的介质恢复强度的计算模型，应用到切除变压器操作过电压的计算中；采用Saber仿真软件模拟计算真空断路器切除变压器操作过电压；并分别对真空断路器切除空载及负载变压器操作过电压的特点与影响操作过电压的各种因素做了详细的分析；最后简要分析了真空断路器开断电容器组过电压的形成原因及为减少重燃的电极老炼措施。

DHCC Constance in the chemistry belongs to the onlighted Cyclopentanoperhydrophenanthrene when it reacted together with the acid Constance it could transform into the fluorescence phenol.we did the flouric determination for the DHCC, in which treated by the ethanol-sulfuric acid or acetic anhydride-methylene chloride-sulfuric acid, acquired standard curve in the 0-40μg/ml presented the liner relet...

DHCC类物质在化学上属于不发光的环戎烷多氢菲类，但与酸性物质一起反应时，可转化为产生荧光的酚类。用乙醇硫—酸或乙酸酐—二氯甲烷—硫酸处理后的DHCC进行荧光检测，获得标准曲线0—40μg/ml范围内呈线性关系，检出的最高灵敏度可达300ng/ml。该方法可用于分离后的DHCC及单一种DHCC代谢物的定性及定量分析。

Alchemists made resultless efforts to transform one metal into another.

炼金术士企图把一种金属变成另一金属，这完全是徒劳的。

Alchemists made resultless efforts to transform one metal into another.

炼金术士试图把一种金属变为另一种金属，这完全是徒劳。

When you can match the shape of the bumps with the shape of your turns, you'll transform bump bashing into ballet.-Michael Rogan, Heavenly, Calif.

如果你可以将你需要做的急转弯的方式和雪包的类型完美的协调起来，那么对于你来说，在不同的雪包间穿行就和跳芭蕾一样轻灵自由。

Microorganism not only is a kind of hydrocarbon- generating components participating in sedimentary organic matter formation directly, but also can impel "humic" organic matter to transform into "sapropel" organic matter, so as to improve organic type and raise hydrocarbon-generating potential of source rocks.

微生物不仅本身能做为生烃成分，直接参与沉积有机质形成，而且还可使&腐殖型&有机质转向&腐泥化&，改善有机质类型，提高源岩的生烃潜力。

But only if his worthless assistants could transform them into something more than the shambling wrecks he saw here!

但是在那之前，他这群没用的助手们必须将他们变成比他现在看到的这种呆滞的傀儡更好的东西。

Don not attempt to do something which you can not to do.

不要企图做那些办不到的事情。

The expression of CTGF and TNF-αweredetected by immunochemistry and the number of Clara Cells was calculated.

光镜下观察肺组织的病理变化，采用免疫组化染色观察肺组织中结缔组织生长因子和肿瘤坏死因子-α的表达和Clara细胞的数量。