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After administration of L-N〓-nitro-Arginine, a specific antagonist of NOS, it was found that the basal and stimulated NO release of blood vessels (aorta, internal carotid, renal and mensenteric artery) was decreased further. The increase of coronary blood flow stimulated by Ach was also reduced further, while the platelet aggregation degree and erythrocyte aggregation index increased significantly. Pathological examination indicated that aortic endothelium was destroyed, the blood vessels in cerebral, cardiac and renal tissues were hypertrophied further and some were almost occluded. The above fin dings resulted in SHR feeded with L-NNA having high incidence of stroke rate and high degree of renal cirrhosis when compared to control SHR. The infarct and hemorrhagic focuci in cerebral tissues, infarct focuci in cardiac tissues, atrophy and hyalinedegeneration of renal glomeruli and degeneration even necrosis of renal tubuli in renal tissues were al so found. The increase ratio of brain and heart to body weight and decrease ratio of kidney to body wei ght further demonstrated that the cerebral, cardiac and renal injuries were aggravated.

在上述结果的基础上进一步给予SHR NOS特异性拮抗剂左旋亚硝基精氨酸后，血管(胸主、颈内、肾及肠系膜动脉)NO基础释放及激动剂刺激释放进一步减少，Ach刺激冠脉流量的增加和胸主动脉内膜NOS活性也进一步减少，血小板聚集程度及红细胞聚集指数显著上升，病理切片显示胸主动脉内皮细胞破坏明显，脑心肾组织内的血管进一步增厚甚至几乎阻塞，导致饲L-NNA的SHR缺血性及出血性脑卒中发生率显著高于对照SHR，脑体重比显著升高；心肌内出现明显的缺血梗塞疤痕灶，心体重比显著升高；肾小球明显萎缩、玻璃样变性，肾小管也变性甚至坏死，使肾硬化程度显著增加呈颗粒性固缩肾，肾体重比也显著减少。

Methods: Forty-four specimens of renal tissues were harvested from urolithic patients,control tissues were harvested from non-urolithic patients (6 specimens) and renal tumorpatients performing nephrectomy (21 specimens). Immunohistochemical technique wasestablished to study the location of VKORC1 in renal tissues. Western blot technique wasused to detect the differential expression of VKORC1 protein in renal tissues.

方法收集肾脏皮质组织标本71例，其中草酸钙结石组44例，对照组27例(肾盂输尿管交界处狭窄和输尿管结核组6例，皮质厚度小于1.0cm；肾肿瘤皮质组21例，皮质厚度大于1.0cm)，应用免疫组织化学法检测VKORC1在组织中的表达部位，应用免疫印迹蛋白半定量法，检测各组VKORC1蛋白的表达，并分析其表达与肾脏积水及皮质厚度等因素的关系。

There are distinctdifferences in the distribution and metabolism in the rat tissues after ivadministration of the two epimers of GL. After iv administration,α-GL istarget to the liver, and can be efficiently converted to more glycyrrheticacid thanβ-GL, and it nearly has no accumulation in the tissues.The distribution ofβ-GL in rat tissues appears to be wide throughout thebody, and its metabolism is slower than that ofα-GL to accumulating insome tissues.

结果显示α-GL与β-GL在大鼠体内的组织分布存在显著性差异，大鼠尾静脉注射α-GL后在体内呈肝分布特异性，转化成甘草次酸的速率高于β-GL，无组织蓄积；而β-GL在体内分布广泛，代谢较慢，有蓄积的可能。

Between P14 and P21,CIAPIN1 immunoreaction in the brain,heart and liverbecame much lower. However,between P21 and P28,CIAPIN1 immunoreactionin the heart,brain,liver and skeletal muscle became much lower,while with thekidney development,CIAPIN1 immunoreaction in the kidney became higher. Invarious tissues from adult mouse,CIAPIN1 immunoreaction could be seen incardiac muscle cell,brain,hepatocyte,epithelium of renal tubule,skeletal muscle,lung tissue,gastric mucosa and gland,acinus lienalis.2. Distribution of CIAPIN1 in normal fetal and adult human tissuesTo reveal the possible physiological role of CIAPIN1,we examined theexpression and distribution of CIAPIN1 in fetal and adult human tissues usingimmunohistochemistry. We found that CIAPIN1 was ubiquitously distributed infetal and adult tissues,and was localized in both the cytoplasm and the nucleus.

然而，在3个月大的成年鼠中，CIAPIN1阳性反应物在心、脑、肝和肾小管中的表达强度要低于P28小鼠；但CIAPIN1阳性反应物在成年鼠骨骼肌中较P28小鼠高。2、CIAPIN1蛋白在人5个月胚胎及成人多器官组织内的表达在人5月胚胎多器官组织中，CIAPIN1阳性反应物见于心脏、胆囊单层柱状上皮和粘膜、结肠粘膜、小肠粘膜和绒毛、肝脏、直肠腺体、胃粘膜、肾上腺束状带、甲状腺滤泡、脾索、胸腺小叶间隔、皮肤真皮层和汗腺、睾丸白膜和间质、脑组织内神经元和神经胶质、肺小支气管和肺泡、骨骼肌、肾脏皮髓质和肾小管、子宫内膜、胰腺腺泡和胰岛、卵巢、输卵管粘膜等绝大多数组织细胞。

The fluence and frequency of laser was controlled precisely, which could melt and destroy the adiposis tissue without damage to the adjacent tissues.The histological study of treated adiposis tissues,post-operative reaction and clinical results were observed and studied.Results Local adipose tissues were melted and liquefied effectively by the laser.

方法对35名面颈部及上臂局部脂肪堆积的患者进行了激光融脂术，使用Nd：YAG激光经光纤导入皮下脂肪层，精确控制激光的输出能量和频率，利用激光的热作用融解脂肪组织，观察脂肪组织的组织学变化、术后反应及临床效果。

Allantois and surrounding tissues of 8.5 day post coitum embryos, the hindhut and surrounding tissues of 9.5 dpc and 10.5 dpc embryos, the gonadal ridges and surrounding tissues of 11.5 dpc and 12.5 dpc embryos were collected and digested with 0.25%pancreatin-0.02%EDTA, then the cells were cultured in the plastic petridishes which are pretreated with 0.1% gelatine.

实验方法：取胚龄8.5 d胎鼠尾端尿囊及周围组织，取胚龄9.5~10.5 d胎鼠后肠及周围组织，取胚龄11.5 d，12.5 d胎鼠生殖嵴及周围组织，分别经0.25%胰酶-0.02%EDTA消化并接种于0.1%明胶包被的培养皿中。

Materials and Methods:236 pathology-confirmed breast lesions(155 malignanty,81 benignancy) and 221 disease-free contralateral breast tissues were examined using EPI-DWIb values=0,1000 s/mm~2TR/TE=8400ms/93.8ms; FOV=30×30cm;matrix=128×128;ASSET=2,slice thickness=4mm.DCE-MR imaging was also performed for accurate location of the lesion using VIBRANT sequence.The mean ADC values of malignant and benign lesions and the disease-flee contralateral breast tissues were calculated from the ADC map for each patient.One-way analysis of variance was applied to compare the mean ADCs of malignant lesions with benign lesions and disease-flee contralateral breast tissues.The threshold ADC value for malignant lesion was determined using a receiver operating characteristic(ROC curve analysis.Each lesion was classified into two major types:mass and non-mass lesion.The diagnostic performance of DWI was compared in breast mass and non-mass lesions.Results:155 malignant and 81 benign lesions were confirmed in 236 lesions.

材料与方法：搜集术前行乳腺MR检查并经穿刺或手术病理证实的236例乳腺病变，以对侧正常乳腺腺体作为正常对照，采用横轴位的平面回波-扩散加权成像序列，TR=8400ms，TE=93.8ms，层厚=4mm，激励次数=2：视野=30×30cm，矩阵=128×128，ASSET=2，b值=0和1000s/mm~2；参考动态增强图像准确定位，测量病变区和对侧正常乳腺腺体的ADC值，应用t检验比较良、恶性病变及正常腺体ADC值的差异，采用接收者工作特征曲线(receiver operating characteristic curve，ROC)确定良恶性病变的ADC界值；根据BI-RADS MRI将乳腺病变按照形态学表现分为肿块性病变和非肿块性病变两组，比较ADC值在肿块和非肿块性病变中定性诊断的效能。

RESULTS: The collapse was obvious at weeks 2 and 6 after modeling, with only few granulation tissues and fibrous tissues. No chondrocytes were observed. At weeks 12 and 18, fibrous tissues were found at the defect site, and bone tissue and cartilage proliferation was observed at the bottom and margin. However, it was greatly different from normal cartilage. Moreover, there was still no chondrocytes.

结果：实验兔后腿关节的股骨髁关节面的关节软骨缺损后，在制模后2，6周缺损部位凹陷明显，只有少许肉芽组织和纤维组织生长填充，未见软骨细胞爬行生长，12，18周见缺损部位以纤维组织增生为主，底部及边缘可见骨组织和软骨增生，但是镜下形态仍与正常软骨组织区别较大，表面仍未见软骨细胞爬行生长。

Methods Fifty pairs of fresh colorectal cancer and homologous normal tissues were genotyped with Identifiler Kit and the mutations generated in cancer tissues were determined. The mutation rates, the numbers of locus matched without identical allele (A0), 1 identical allele (A1), or 2 identical alleles (A2) and the number of total identical alleles IA(subscript n were calculated. Frequency distributions of A0, A1, A2 and IA were compared among CR-N group, unrelated individual pairs and full sibling pairs. Discrimination functions were established for individual identification from tumor tissues with discriminatory analysis.

用Identifiler系统对50对新鲜结直肠癌组织及其身源正常组织组进行STR分型，计算CR-N组中变异STR基因座及全不同基因座数(A0)、半相同基因座数(A1)、全相同基因座数(A2)和共有等位基因数IA(下标 n，比较CR-N组、无关个体对组和全同胞对组中上述参数的分布差异，通过判别分析建立判别函数。

He expression rate of TGF-β1 in human suprarenal epithelioma tissues was significantly higher than that in the corresponding tumor- adjacent tissues (32.56% vs. 0%). TGF-β1 was expressed in grade and staage Ⅰ or Ⅱ suprarenal epithelioma tissues.

GF-β1在肾透明细胞癌组织中的表达阳性率为32.56%(14/43)，而癌旁组织均无表达；TGF-β1表达阳性的肾透明细胞癌病理分级和临床分期均处于Ⅰ或Ⅱ级。

The system can be widely used in belt weighing and batching process control,etc.

介绍了基于智能模糊控制的嵌入式配料系统的设计方法，对模糊PID控制过程和系统调零作了重点的讨论，同时给出了PID控制的程序流程图和系统定长调零子程序。

"I dunno," she answered,"but if you show me aroun', I'll recognize it from the lighthouse just outside the porthole."

"我不知道，"她回答说，"如果你能带我转转，我能认出来的，因为灯塔就在舷窗外。"