查询词典 tissue culture

Using the tissue cultured bulblet of narcissus cultivar Fortissimo as tested materials, with 1/2MS as the basic medium, 4 conch, of NAA and IBA including 1.0, 0.5, 0.1 and 0.05 mg/L were set up to study the effects of hormone on rooting induction of tissue culture seedlings in narcissus.

方法］以水仙栽培品种Fortissimo的组织培养小鳞茎为试验材料，以1/2MS为基本培养基，设计1.0、0.5、0.1，0.05mg/L 4个NAA、IBA浓度，研究激素对水仙组培苗生根诱导的影响。

Lolium perenne ; tissue culture ; callus tissue ; carbon source ; auxin

多年生黑麦草；组织培养；愈伤组织；碳源；生长激素

Hemerocallis fulva ; tissue culture ; callus tissue

大花萱草；组织培养；愈伤组织

Ournal of Applied Phycology publishes original articles relevant to practical uses of algae, including fundamental research, development of techniques and commercial applications. The scope includes algal biotechnology and genetic engineering, tissue culture, culture collections, commercially useful micro-algae and their products, marine culture, algal inoculation and soil fertility, pollution and fouling, monitoring, toxicity tests, toxic compounds and antibiotics.

应用藻类学杂志》发表与藻类实际应用相关的原始文献，包括藻类学基础研究，技术开发和商业应用，范围涉及藻类生物技术，遗传工程，组织培养，培养物收集，适用于商业性利用的微藻及其产物，海水培养，藻类接种和土壤肥力、污染和发臭，监测，毒性试验，有毒化合物，抗菌素。

Suitable control content of culture medium can benefit growth, proliferation rate of Williams B6 seedling, save spending of production material. The content of 30ml per bottle show the highest proliferation rate and the higher usage of culture medium, the contrary result will appear when preponderate over the content of 30ml per bottle, but the increase weight of single seedling. For the losing of medium by sterilization, seedling SO2 resistance and other effects, it's considered the content of 25~30ml per bottle is fit for production of tissue culture seedling.

适宜控制培养基用量，能稳定威廉斯B6香蕉组培苗生长量、繁殖率，减少原料投入。30ml的用量其增殖率最高、培养基利用率较高，超过30ml的用量其增殖率相对下降，培养基利用率降低，但单苗增重量最大，综合考虑培养基损失、蕉苗耐SO2性等因素，认为培养基用量应控制在25~30ml/瓶。

According to the target culture, tissue culture can be divided into organ, cell suspension and protoplast culture.

据所培养的对象，组织培养可分为器官、细胞悬浮和原生质培养。

For the bud directly differentiation , the suitable culture medium was MS+BA 2mg/L +NAA 0.2mg/L or MS+TDZ0.01mg/L +NAA0.2 mg/L, of which the induction rate was 88% and 100% respectively. An efficient tissue culture system has been developed with the bud of mature seed of Aesculus hippocastanum L. as explants . Buds were induced from 2cm high young plantlet cultured on MS medium supplemented with 0.6 mg/L 6-BA plus 0.1mg/L or 0.4~0.6mg/L ZT plus 0.1 mg/L NAA for 15 d, and the induction rate was 100%, the mean No. of buds was 35.7; The combination of MS+0.2mg/L 6-BA +0.1mg/L NAA + 10mg/L AD was the suitable culture medium for elongation of the buds.

以欧洲七叶树成熟种子的胚芽进行离体培养和快速繁殖，结果表明：高约2cm的无菌苗在MS+0.6mg／L 6-BA+0.1 mg／L NAA或MS+0.4～0.6mg／L ZT+0.1 mg／L NAA培养基上培养15 d左右可诱导出不定芽，分化频率为100％，平均每株产芽35.7个；MS+0.2mg／L 6-BA+0.1mg／L NAA+10mg／L AD培养基有利于芽伸长；生根培养基为1／2MS+0.4 mg／L NAA+0.2mg／L IBA时，生根率可达75％。

The phenomenon of vitrification could restore significantly after culture 2 or 3 generations in the culture medium without hormone. The differentiation ability of protocorm restored and the state of plant growth was consistent with the formal protocorm. It could continue applying to the manufacture of tissue culture.

玻璃化类原球茎在无植物生长调节剂培养基中经2~3代恢复培养后，数量下降到0.84%，玻璃化现象可得到明显恢复，恢复后的类原球茎的分化能力和植株生长状态与正常类原球茎一致，无变异现象发生，可继续应用于组培生产。

METHODS: Using tissue culture technique, the maximal non-toxic concentration of luteolin in swine testicle cell culture was measured, and the inhibitory effect of luteolin on TGEV was measured by cytophathogenic effect observation and crystal violet measurement. In addition, the degrees of inhibition of the luteolin on CPE of virus in cell culture were observed by changing sequence of adding the vires and the drag. RESULTS : The maximal non-toxic concentration of luteolin was 873.39 μmol/L.

采用细胞培养法，通过结晶紫摄入量测定结合细胞病变作用，观察木犀草素在不同的加药方式下，对感染TGEV的猪睾丸细胞的保护作用、结果：TGEV病毒先吸附ST细胞再加入药液，先与TGEV病毒孵育再接种于卯细胞，或药液先与细胞孵育再用病毒感染等不同方式下，木犀草素在0.84-54.50μmaol／L浓度范围内，均能明显地提高细胞的存活数。

The research on the tissue culture and cell suspension culture showed that thesuitable culture medium for inducing bud was MS supplemented with 1.5mg/L 6-BAand 0.1mg/L NAA, and for the generation-continuing multiplication was MSsupplemented with 1.5mg/L 6-BA and 0.2mg/L NAA. The rooting medium was1/2MS supplemented with 0.5mg/L IBA and the rooting rate was 45.0%. Plantletsurvival after transfer to sand was 52.5%.The induction rate of calli was66.7%～86.6% and the optimum medium was MS medium with 0.5mg/L 6-BA and2.0mg/L 2,4-D. The calli became smallest partical size, friable and had gooddispersion ability after 3 times successive transfer culture, the optimum medium wasMS medium with 0.2mg/L 6-BA and 2.0mg/L 2,4-D. Culturing these particles on sixkinds of MS liquid media with different hormone contents, the optimum medium wasselected basing on he change of the density of single-cell, the density of cellaggrefate and the mass of cell.

对蒜头果进行的组织培养与细胞悬浮培养研究结果表明：MS+6-BA1.5mg／L+NAA0.1mg／L+蔗糖3％激素组合能够较好地诱导芽的初始分化和增殖，适宜的芽苗继代增殖培养基为MS+6-BA1.5mg／L+NAA0.2mg／L+蔗糖3％；采用1／2MS+IBA0.5mg／L+蔗糖3％为生根培养基，生根率为45.0％；移栽到河沙的生根苗成活率为52.5％；愈伤组织诱导率为66.7％～86.6％，其中以MS+6-BA0.5mg／L+2，4-D2.0mg／L+蔗糖3％的培养基最佳，其诱导出的愈伤组织具有较强的增殖能力和较好的脆散结构，最佳继代培养基为MS+6-BA0.2mg／L+2，4-D2.0mg／L+蔗糖3％，且培养基中的6-BA与2，4-D浓度的比值越小，愈伤组织生长越快，结构越脆散，增殖率越高；将继代后的愈伤组织转入6种含不同激素浓度组合的MS液体培养基中进行振荡培养，在综合分析各培养基的单细胞密度，细胞团块密度，细胞生物量增长率等指标后，初步筛选出MS+6-BA0.2mg／L+2，4-D2.0mg／L培养基为较好的液体培养基。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。