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DNA were abstracted by means of phenol chloroform, amplification and cataphoresis test of polymerase chain reaction of target DNA,primer sequence of gene segment amplification of 5-hydroxytryptamine (5-HT) 2A receptor gene T102C: 5'-TCT GCT ACA AGT TCT GGC TT-3', 5 '-CTG CAG CTT TTT CTC TAG GG-3'; Reaction system of 50 μL, DNA 0.5 μg,Primer 50 pmol,TagDNA enzyme 2U were added with dNTP to the final concentration of 200 μmol/L.

应用常规苯酚氯仿法抽提DNA，目的DNA的聚合酶链反应扩增及电泳检测，5-羟色胺2A受体基因T102C基因片断扩增的引物序列：5'-TCT GCT ACA AGT TCT GGC TT-3'。5'-CTG CAG CTY TTT CTC TAGGG-3'；采用50 μL反应体系，DNA 0.5 μg，引物50 pmol，TagDNA酶2U加dNTP至终浓度200μmol/L。

Admin / July 8th, 2009 / No Comments Posted in Cenacle , Marian , Prayers , Reflection , Sharing / Tags: no tag

耶稣向她说：『我也不定你的罪；去罢！从今以后，不要再犯罪了！』若8：2-11

The recombiant protein with GST tag was soluble. SjMRLC showed similar transcription level in all stages including sporocyst, miracidium, egg, cercaria, schistosomulum, female worm, male worm, and paired adults.

结果 构建pGEX 4T-3/SjMRLC重组质粒，测序表明插入的外源基因片段正确，诱导表达获得带有GST标签的可溶性蛋白（Mr 49 000）；虫期转录特异性分析显示该基因在日本血吸虫各个虫期均有转录，且水平一致。

He will also wear an electronic tag and live under vitual house arrest in his chalet in the Swiss Alps.

另外他将佩戴一个电子标签，并且实际上被软禁在他在瑞士阿尔卑斯山的木屋中。

But the chalkboard look does tack on another 20 Gs to its price tag.

但黑板一样的外观也在它的价格标签上增加了 2 万块钱。

If Warner Chappell's worth is pegged at between $1.2-1.5 billion, the stated $1.5 billion value of BMG Music Publishing would seem inflated, with the real price tag somewhere south of $1 billion.

业内人士估算，作为全球第二大音乐公司和第三大音乐发行公司，Sony BMG和BMG音乐发行合计价值在35亿至40亿美元之间，而GBL的这部分股权则应在60亿美元上下。

In February 2000, the following flow charts and recording sheets were improved and put into use in 2001 by the former General Administration of Inspection and Quarantine: the whole CIQ residue monitoring work flow chart, CIQ residue monitoring sampling flow chart, CIQ residue monitoringsampling sheet, CIQ residue monitoring sampling tag, CIQ residue monitoring delivery sheet, CIQ residue monitoring testing results report sheet and the result statistics table.

2000年2月原国家出入境检验检疫局制定并修订完善了《中国动物及动物源食品残留物质监控计划》的工作流程图、CIQ残留监控取样流程图、CIQ残留监控取样单、CIQ残留监控样品标签、CIQ残留监控送样单、CIQ残留监控检测结果报告单、CIQ残留监控结果统计报表，并在实施2001年度残留监控计划中启用。

In fact, these seem to come free when you check it's price tag.

事实上，这似乎是免费的，当你检查它的代价。

This custom gamer tag shows COD4 with a soldier in chemical warfare gear.

这种风俗游戏标签显示COD4与一名士兵在化学战装备。

It was just Tag, you know, like Cher or..

只是叫泰格而已，你知道的就像雪儿或是

The small blue capacitor has very high chances to go fully shorted compare to electrolytic capacitor which is very rare.

小蓝电容器具有很高的机会去充分地缩短了比较电解电容器，这是非常罕见的。

In the Italian campaigns of 1513 and 1515, when the Swiss won the victories of Novara and Marignan, he acted as army chaplain.

在意大利的运动， 1513年和1515年，当瑞士赢得了胜利，诺瓦拉和marignan ，他担任军队的牧师。