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The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

But one day our priest, Father Ivan, came and drank up all my liquor at one sitting; and the Vyestnik Evropi went to the priest's daughters; as in the summer, especially at the haymaking, I did not succeed in getting to my bed at all, and slept in the sledge in the barn, or somewhere in the forester's lodge, what chance was there of reading?

然而一天，我们的牧师伊凡神父来了，一口气喝完了我所有的酒，Vyestnik Evropi也到牧师的女儿们手里去了。夏季，特别是割晒牧草的时候，我根本连床都挨不到，有时睡在谷仓的雪撬上，有时睡在某个森林人的小屋里，哪还有看书的机会？

But one day our priest, Father Ivan, came and drank up all my liquor at one sitting; and the Vyestnik Evropi went to the priest's daughters; as in the summer, especially at the haymaking, I did not succeed in getting to my bed at all, and slept in the sledge in the barn, or somewhere in the forester's lodge, what chance was there of reading Little by little I moved downstairs, began dining in the servants' kitchen, and of my former luxury nothing is left but the servants who were in my father's service, and whom it would be painful to turn away.

可是一天，我们的牧师伊凡神父来了，一口气喝完了我所有的酒，Vyestnik Evropi也到牧师的女儿们手里去了。夏季，特别是割晒牧草的时候，我根本连床都挨不到，有时睡在谷仓的雪撬上，有时睡在某个森林人的小屋里，哪还有看书的机会？慢慢地我搬到楼下来了，开始在仆人的厨房里吃饭，除了我服侍父亲的仆人，解雇他们会令他们痛苦万分，我之前的奢侈荡然无存。

But one day our priest, FatIT Ivan, arrived and drank up all my liquor at one sitting; and the Vyestnik Evropi go to the priest's daughters; as in the summer, especially at the haymaking, I did not succeed in getting to my bed at all, and slept in the sledge in the barn, or somewITe in the forester's lodge, what chance were tITe of reading?

可是一天，咱们的牧师伊凡神父来了，一口气喝完了我所有的酒，Vyestnik Evropi也到牧师的女儿们手里去了。夏季，特别是割晒牧草的时间，我根本连床都挨不到，有时睡在谷仓的雪撬上，有时睡在某个森林人的小屋里，哪还有看书的机遇？

This is not just a popcorn movie, it shapes up the look of modern audiences in pint sized ways and is loved by all who watch because of its brilliantly constructed humour and heartbreakingly dramatic scenes, the movie with nearly every movie genre squeezed in, weather it be a comedy, or a drama, or a romance, or a war movie, or an epic, or a fantasy, or whatever, you don't have to be a fan of a particular genre to love this movie, I am a lover of all sorts of genres, from westerns to samurai movies, but this movie got to me mostly because I found it to be the best feel good movie I can remember, so when you are pinned with a choice of this movie against any other feel good classics, its hard to turn down Forrest Gump, which succeeds in areas where other comedy dramas have failed to ignite, but Forrest Gump doesn't just succeed in one area in particular, its succeeds on quite a few, and they are so noticeable in the final draft, that this is instantly re watchable.

这不仅是爆米花电影，它的形状了期待的现代观众在一品脱大小的途径和被爱是所有与会人士观赏，因为它精辟地兴建的幽默感和heartbreakingly戏剧性的场面，电影几乎每部电影体裁挤在，它天气预报是一个喜剧，还是一台戏，或浪漫，或战争电影或一部史诗，或幻想，或不管，你不须风扇某一流派，以热爱这部电影，我是一个情人各种流派，从西部来的武士电影，但这部电影让我吓到主要是因为我发现这应该是最精彩的电影，我可以请记住，所以当你寄托与选择这部电影针对其它任何感觉很好经典，其强硬拒绝阿甘正传，其中成功的地方其他喜剧电视剧未能点燃，但阿甘正传不只是成功的一个领域，尤其是它的成功上也有不少的，他们是如此显着最后草案，这是即刻重新值得一看的。

Staffordshire University MBA programme, which is focus on accountancy learning, provides a professional accountant theory, Analysis and prepare Cash Flow, Analysis Balance Sheet, Pay Back Period, Risk Management and etc; apply all these in the real business, very useful to use in the company and give us a whole mirror what we learned and how we used in the business; provides a holistic understanding of the theory and practice of management and the up-to-date knowledge and skills needed to succeed in the real business world.

英国斯坦福厦大学工商管理课程包括：会计学，经济管理，统计学，战略管理，世界市场营销，调查方法，销售技巧，运作管理等等。斯坦福厦大学的MBA课程侧重于财务管理，熟练掌握了会计的基本技巧和实践应用，能熟练应用资产管理，财务月分析报表，成本分析，风险分析，现金流量，资本回收期等。

In this paper,we chosed the Piranha package LED as lighting sourse,and designed the reflector cavity and pillow lens,and built the 3D model of tail lamp in the structure software Catia,and obtained the optical simulation results of tail lamp in the optical simulation software TracePro,and we succeed in developing a LED tail lamp in last;After the testing in automotive lamp center,it was proved that our lamp achieved the national standard of automotive lamps.

摘 要：采用食人鱼系列的发光二极管(light em itting d iodes，简称LED)作光源，进行枕型透镜与反射腔的设计，在结构软件Catia中建立了一款后位灯的三维模型，并利用光学软件T raceP ro进行光学效果的仿真，开发出了一款LED后位灯。样品经汽车灯具测试中心检测，性能满足国家标准要求。

In the artificial rearing larval honeybee method, 1-day-old larvae were reared in laboratory and 0.1 - 100 ppm pyriproxyfen was added in larval food. A dose respondent effect was found in the larval development of the treated larvae. Results showed that 0.1 ppm pyriproxyfen reduced the eclosion rate of the reared larvae conspicuously, and 1 ppm pyriproxyfen reduced that 84.6% of eclosion honeybee appeared the condition of deformed wings. The 10 ppm pyriproxyfen treatment caused 53.8% melanic death in the pupal stage, and no honeybees succeed in eclosion.

在室内饲养幼虫方面，分别添加0.1-100 ppm百利普芬於幼虫食物中，结果显示0.1 ppm百利普芬即会显著降低饲养幼虫的羽化率，而且添加1 ppm百利普芬会有84.6%羽化蜜蜂出现残翅现象；添加10 ppm百利普芬则会造成53.8%蜜蜂在蛹期黑化而死亡，而且无蜜蜂羽化成功；100 ppm百利普芬则有50%蜜蜂在幼虫期死亡，50%蜜蜂在蛹期死亡，亦无蜜蜂成功羽化。

The Lynn White debate, which begun when White called for a rethinking of Christianity or its replacement by a nonWestern religion, has proved an obstacle to dealing with the environmental crisis The defense of Christianity that followed inhibited Asian and comparative environmental ethics in the West and did not produce environmentally useful results Whenever the relationship of religion and the environment was raised, the discussion quickly shifted to the issue of Christian responsibility for the environmental crisis The problem with the debate is that in many important ways, the White thesis is correct: Christianity is indeed responsible to a significant degree in that Christianity desacralized nature and it supported policies politically in the Middle Ages that encouraged now harmful human population growth and promoted science and technology, much of which has proved to be environmentally problematic There was nevertheless a second tradition of which Saint Francis of Assisi was a part that was environmentally friendly and that could be useful in rethinking Christianity environmentally It is however unlikely that Christianity will prove useful in the development of an environmental ethic in nonWestern countries or that nonWestern religions will succeed in producing an environmental ethic in the West It is therefore important that religious scholars move beyond assigning blame for the environmental crisis and find ways for the major world religions to help develop environmental ethics as best they can within their own cultural spheres

林恩怀特争论始于怀特关于对基督教进行重新思考或者由非西方宗教取而代之的呼吁。林恩怀特争论已经被证明成为处理环境危机的一个障碍。由此而起的对基督教的辩护制约了亚洲和其他比较环境伦理学在西方的发展，没有产生对环境有益的结果。无论何时，当人们谈及宗教和环境的关系的时候，讨论都会迅速转向有关基督教对环境危机责任的讨论。这一争论的关键在于，怀特的论文在很多方面是对的：基督教的确在相当大的程度上造成了自然的世俗化，并且在中世纪，基督教在政治上对现在看来有害的人口增长的政策提供了支持，促进了科学和技术的发展，很多诸如此类的政策已经被证明对环境是有害的。但是，基督教还存在另一种对环境友好的传统，Assisi城的&圣芳济&运动就是其中一例，这种传统也许对于从环境角度重新认识基督教是有益的。但是，对于非西方国家环境伦理的发展，基督教将很难能够有所作为，非西方宗教也不大可能成功地在西方产生出某种环境伦理。因此，对于研究宗教的学者们而言，重要的是要走出谁应该为环境危机受到指责的争论，找到世界各主要宗教如何在其各自的文化范围内对发展环境伦理提供最佳帮助的方法。

What in hell's success if it isn't right there in your Stevenson sonnet, which outranks Henley's 'Apparition,' in that 'Love-cycle,' in those sea-poems? It is not in what you succeed in doing that you get your joy, but in the doing of it.

成功！你的成功已经在你的《爱情组诗种为斯蒂文森写的那首十四行诗里了，已经在你那些海洋诗里了。

Methods: Five patients with parkinsonism or dystonia were assigned to general anesthesia using an modified endotracheal tube.

本实验依照人体实验之相关规定进行，五位患有帕金森氏症或肌张力异常的病人接受神经立体定位手术。

If you can benefit from this book, it is our honour.

如果您能从本书获益，这将是我们的荣幸。