查询词典 substitution method

On the other hand,the Inverse PCR combined with a tail-to-tail primer design requires T4 DNA ligase enzyme to ligate PCR products,which makes the positive rate relatively low.We aim to find out an easy and effective method to perform gene deletion, insertion and substitution mutation.

近几年，有文献报道使用基于Quikchange原理的方法来制作DNA片段的插入、替代以及删除突变，但由于QCM-PCR的低产量，难以产生阳性克隆。

METHOD Dofetilide was synthesized by steps of reaction (substitution ,reduction, methylsulfonylation and methylammoniation, etc) from p-nitrophenol and p-amino phenethyl alcohol.

以对硝基苯酚为起始原料，经取代、还原、甲磺酰化等反应制得 4甲磺酰苯胺(5 )；以对氨基苯乙醇为起始原料，经甲磺酰化、甲氨化等反应制得 4 甲磺酰苯胺(8)，再与5经取代反应制得多非利特。

In order to improve the electrochemical performance of LiCoO2, the majorities of researchers are working on the adjustment and substitution of the lay element, in order to increase the extract and deintercalated quantity and overcharge-resistant ability, the method pillaring in the interbed is brought forward for strengthening the structure stability.

目前为了改善LiCoO2的电化学性能，多数研究者进行的是层板元素的调控和替换，但实际效果仍不甚理想，我们采取层间柱撑金属离子的办法来提高材料的结构稳定性，以增加LiCoO2材料的锂离子可脱嵌量以及耐过充电压。

In order to improve the electrochemical performance of LiCoO2, the majorities of researchers are working on the adjustment and substitution of the lay element, in order to increase the extract and deintercalated quantity and overcharge-resistant ability, the method pillaring in the interbed is brought forward for strengthening the structure stability.

目前为了改善LiCoO2的电化学性能，多数研究者进(来源：8272A27BC论文网www.abclunwen.com)行的是层板元素的调控和替换，但实际效果仍不甚理想，我们采取层间柱撑金属离子的办法来提高材料的结构稳定性，以增加LiCoO2材料的锂离子可脱嵌量以及耐过充电压。

A single crystal complex [Ni2(H2O)2]2DMSO(Hqina=quinaldinic acid, DMSO=dimethyl sulfoxide) was synthesized at room temperature by the solvent substitution method, which belongs to monoclinic system with space group C2/m.

在常温下，通过溶剂置换法制备了[Ni2(H2O)2]2DMSO单晶，其中qina为喹哪啶酸根，DMSO为二甲亚砜。

Compared with repatch technique and intracellular solution substitution techniques used abroad, this method operates more easily and can produce more reliable results.

此法和二次钳压技术及国外介绍的微插管电极内液置换法相比，更加简便易行，结果更可靠。

Carboxymethyl starch was prepared from cornstarch by using a dry method,and the molecular structure,granule configuration and crystallizability of the carboxymethyl starches with different substitution degree were investigated by using ultrared spectrograph,electro scanning microscope and x-ray crystallograph.

张慧，董海洲，侯汉学，刘传富，干法羧甲基化反应对玉米淀粉颗粒性质影响的研究以玉米淀粉为原料，干法制备羧甲基淀粉，并利用红外光谱仪、扫描电子显微镜和X-射线衍射仪对不同取代度的羧甲基淀粉的分子结构、颗粒形貌及结晶性进行了研究。

Methods: The target compounds were designed and synthesized by inletting different substitution amino groups using acetyl fragment as the connecting segment.Born method was used for preliminary pharmacological test in vitro.

以乙酰基为连接片段，引入不同的取代氨基，设计合成一系列化合物；体外药理实验参考Born方法进行。

The thermokintics of nucleophilic substitution reactionsof benzoyl chloride and oxidation reaction of isopropanol by NBS have been studiedby using the mathematical models of the thermokinetic time-parameter method forsimple and consecutive first order reaction, respectively. The kinetic mechanisms ofstudied reactions have been discussed reasonably.

利用简单和连续一级反应的时间参量法的数学模型，分别研究了苯甲酰氯亲核取代反应和NBS氧化异丙醇反应的热动力学，对反应的机理进行了合理的解释和讨论。

The wild grass, bush community, artificial mixed forest were regarded and natural secondary forest were regarded as the research subject of different stages in the process of ecological construction aimed at soil and water conservation around Napahai Lake in the northwest of Yunnan Province. The change of those community structures was studied by using spatiotemporal substitution method.

在滇西北高原香格里拉县纳帕海周边区域，以荒草丛、自然恢复灌丛、自然恢复高山松次生林以及人工混交林等四种生态系统类型为研究对象，采用时空互代法，视它们为水土保持生态修复过程中不同阶段，研究不同生态系统的群落结构变化规律。

If you were not , and OS X just booted normally, have no fear.

如果你没有，和OS X刚刚启动通常，没有恐惧。

I only want to preserve our all .

我只想保存好我们的一切