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Methods TASMCs were stimulated with LPS in the presence or absence of CCK-8 and/or PKC inhibitor chelerythrine, morphine,-OR mRNA expression was analyzed by RT-PCR 16h after stimulation.

培养大鼠TASMCs，在加入或不加入CCK-8和/或CHE、吗啡的情况下，用LPS刺激细胞16h，用半定量RT-PCR检测κ阿片受体mRNA的表达。

The chondroblast cultured with compound porous materials PDLLA and PDLLA/chitosan. The aim are: 1 To examine the proliferation ability and potential chondroblast differentiation; 2 To find an ideal condition stimulated BMSC differentiate into chondroblast; 3 To examine the chondroblast proliferation in porous scaffolds and to explore the interaction between cells and materials; 4 To examine the release of cytokine in vitro.

目的在于：（1）通过骨髓基质干细胞的原代分离、培养及成软骨诱导，观察原代细胞扩增能力及其向成软骨方向分化的潜能；（2）寻求骨髓基质干细胞向成软骨方向分化的理想诱导条件；（3）观察经成软骨诱导的MSC在PDLLA、PDLLA／壳聚糖多孔载体上三维的增殖状况，探求细胞与材料间的相互作用；（4）观察经成软骨诱导的MSC在体外三维培养系统中细胞因子的释放状况，了解维持诱导细胞软骨表型的合适条件及作用机制。

The aim are:lTo examine the proliferation ability and potential chondroblast differentiation ;2To find an ideal condition stimulated BMSC differentiate into chondroblast;3To examine the chondroblast proliferation in porous scaffolds and to explore the interaction between cells and materials;4To examine the release of cytokine in vitro.

取健康成人略后上棘处骨髓 5ml，经 percoll液分离后密度梯度离心，10'砌l密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心 5分钟，10V加 l接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质QCM）中的蛋白多糖，免疫细胞化学染色检测ECM中＊胶原的蛋白合成，RT干CR鉴定诱导细胞＊胶原mRNA的表达。

Nerve endings are stimulated by capsaicin and release CGRP which has positive chronotropic response to cardiac muscle cells.

神经末梢受到辣椒素的刺激后，神经递质CGRP的释放对心肌细胞具有正性变时反应。5。

We conclude that baicalein, wogonin and chrysin show a suppressive effect on iNOS gene expression in macrophages stimulated with LPS and the sequence of their suppressive effect intensity is chrysin, baicalein and wogonin seperately, and that it may be an important foundation for the combination therapy of TCM and Western medicine in the treatment of endotoxin-shock syndrome.

这些的发现，显示baicalein 、wogonin 和chrysin对脂多醣诱导小鼠巨噬细胞株生成一氧化氮有抑制的作用，而以。chrysin抑制作用最强，其次为baicalein 、wogonin，此结果可作为未来中西医整合治疗败血症之主要依据。

In contrast, treatment with baicalein (1OOμM), wogonin (1OOμM) and chrysin (1OμM) increased the formation of TNF-α in macrophages stimulated by LPS (0.1μg/ml).

但相对地，在以脂多醣(0.1μg/ml)诱导巨噬细胞中，以baicalein(100μM)、wogonin(100μM)和chrysin(10μM)处理后，却会增加TNF-α的生成。

Selecting the optimum carbon sources, nitrogen sources and inorganic salt to get the optimum liquid medium recipe by the orthogonal test in three factors and four levers; Isolated toad gastrocnemius stimulated by pulse electricity was regarded as fatigue model and Clitocybe maxima mycelia was compared with Ringer solution to see what influence they had on muscle contract ability.

采用深层培养方法，筛选出较优的碳源、氮源、无机盐，设计三因素四水平的正交试验，筛选大杯伞的最适培养基；采用脉冲式电流直接刺激蟾蜍离体腓肠肌作为疲劳模型，观察大杯伞菌丝体和任氏液对蟾蜍腓肠肌收缩能力的影响。

Methods: The cells were treated with the different concentrations of Cobaltous chloride. The lactic acid dehydrogenase from the supernatant of the cells was detected by a biochemical analysis. The cells were co-transfected with a pGL2-eNOS-p vector while stimulated with Cobaltous chloride at the different concentrations and disposing time, and the transcription activity of human eNOS promoter was determined through using a double luciferase reporter gene system.

用含不同浓度氯化钴的培养基培养细胞，检测细胞培养上清中的乳酸脱氢酶含量；将已构建的pGL2-eNOS-p质粒转染HUVEC-12细胞，利用双荧光素酶报告基因技术检测在不同浓度氯化钴和不同作用时间下的eNOS启动子转录活性。

Further, with a semi-quantitative RT-PCR technique, the temporal expression of UuMAPKKK-like in U. unicinctus coelomic fluid cells was measured after stimulated by sulfide. The mRNA transcript of UuMAPKKK-like was low in the control and short time stressed (2 h, 6 h) groups, up-regulated gradually after 12h stimulation, and then reached its maximum level at 48h.

进一步采用RT-PCR技术对其在硫化物刺激前后的表达进行了检测，结果显示该基因在对照和应激后2h、6h的个体中表达较弱；刺激12h后表达量增高，并随应激时间增加，呈明显上调趋势。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

I want to be a big fish in a small pond.

我要做一条在小池塘里的大鱼。

While the software functioned well, we did not feel the same about their customer support!

虽然该软件运行良好，我们没有同样的感觉他们的客户支持！