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Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

In order to investigate the effect of tumor necrosis factor α on the activation of astrocyte, the oligodeoxynucleotide was used to inhibit the production of TNF α from astrocytes stimulated by Coriaria Lactone.

为进一步探讨 TNF-α在星形胶质细胞激活中的作用，用 TNF-α反义寡核苷酸抑制由马桑内酯(coriaria lac-tone，CL )诱导的星形胶质细胞 TNF-α的产生，观察 NFκBp6 5核转位和 IκBα降解的变化。

Callus derived from petiole explants of Panax japomcus var. major produced three cyanidin glycosides as major anthocyanin pigments in darkness. Anthocyanin synthesis was remarkably stimulated by 2,4-dichlorophenoloxyacetic acid in combination with kinetin in MS medium. The pigment content of the callus reached up to 34. 14 mg/g fw.

从珠子参(panax japonicus var.major)叶柄诱导的愈伤组织在暗培养条件下主要形成3种花色甙色素，且均以矢车菊素为甙元。2，4-二氯苯氧乙酸和激动素的组合能明显地促进色素的合成，色素含量可达34.14mg/g fw。

Callus deried from petiole explants of Panax japomcus ar. major produced three cyanidin glycosides as major anthocyanin pigments in darkness. Anthocyanin synthesis was remarkably stimulated by 2,4-dichlorophenoloxyacetic acid in combination with kinetin in MS medium. The pigment content of the callus reached up to 34. 14 mg/g fw.

从珠子参(panax japonicus ar.major)叶柄诱导的愈伤组织在暗培养条件下主要形成3种花色甙色素，且均以矢车菊素为甙元。2，4-二氯苯氧乙酸和激动素的组合能明显地促进色素的合成，色素含量可达34.14mg/g fw。

Methods The Cynomorium polysaccharide and Cynomorium water extract were extracted and prepared chewable tablets. Wistar rats were randomly divided into normal control, aging model, VitE, Cynomorium polysaccharide chewable tablets and Cynomorium water extract chewable tablets groups. Dgalactose was injected to establish the aging model. After drug intervention, the proliferation activity of rat spleen lymphocytes stimulated by ConA was detected by MTT assay and the spleen index was measured. The pathological morphological changes of spleen were observed. The activity of superoxide dismutase, the content of malondialdehyde and nitric oxide in serum of rats were detected.

提取并制备锁阳多糖、水提取物咀嚼片；Wistar大鼠随机分为空白对照组、衰老模型组、维生素E对照组、锁阳多糖组、水提物组，以D半乳糖建立衰老大鼠模型，药物干预后，MTT法检测各组大鼠脾淋巴细胞对ConA刺激的增殖能力，检测各组大鼠脾脏指数，观察脾组织的病理形态学变化；并检测各组大鼠血清中超氧化物歧化酶活性、丙二醛及一氧化氮的含量。

Total cytoplastic mRNA of PBMC stimulated by anti-CD3 mAb at continuous time points was isolated and reversed to cDNA.

在共培养的12h，IL-2mRNA的含量显著上升并达到峰值14000copies／ml，随着培养时间的延长快速下降。

The scientific study of law stimulated by the Decretum encouraged the papacy to resolve disputed points and supply needed legislation, thus inaugurating the ius novum.

科学学法刺激所decretum鼓励教宗以解决争议点，并提供所需的法例，从而开创了学生联合会novum超过下个世纪的数以千计的教皇decretals发行，并逐步搜集五个

The results indicated that the effects of combination of NAA (0-0.2 mg/L) and 6-BA (0.1 mg/L) can effectively dedifferentiate the vegetative tissue of Hetao apple-pear, and stimulated callus formation or directed growth of leave buds.

试验利用NAA、2，4-D和6-BA对苹果梨叶片进行组织脱分化研究。结果表明，0~0.2 mg/L的NAA与0.1mg/L的6-BA组合可有效促进叶片组织脱分化，形成愈伤组织或直接诱导叶芽的萌发。

However, at the beginning of PVR process, stimulated and activated by the initiative factors, RPE begin to dislocate from Bruchs membrane , migrate, proliferate, dedifferentiate, transdifferentiate and secrete provisinal extracellular matrix proteins, followed by membrane formation and contraction on both surfaces of the neuroretina and in the vitreous body, resulted in tractional retinal detachment.

PVR发生后，RPE受到始动因素的刺激，开始活化，脱离原位，去分化，移行、增生、发生表型转化等，并分泌细胞外基质(extracellular matrix， ECM)，最终在视网膜前后表面和玻璃体内形成具有收缩能力的增生膜，造成牵拉性视网膜脱离。

EGF remarkably stimulated the dermal sheath fibroblasts, dermal Tibroblasts and demal papilla cells. Effection on the dermal sheath fibroblasts was the strongest. bFGF, GM-CSF, insulin and hydrocortisone had not effective on them.

EGF对真皮鞘细胞、真皮成纤维细胞和毛乳头细胞有显著的促生长作用，其中对真皮鞘细胞的作用最强，而bFGF、GM-CSF、胰岛素+氢化可的松对三种细胞没有明显的作用。

This paper designs the communication system of radio telescope FAST.

针对500m口径球面射电望远镜FAST分布式控制系统的特点完成了其通信系统的设计。

Political sheep's step-by-step a large number of fossils unearthed in the county soil Ma Ali after rural village in the middle of sallow pelitic siltstone.

步氏和政羊化石大量出土于广河县阿力麻土乡后山村，产于灰黄色泥质粉砂岩中。