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ML cord blood was collected sterilely, and then anticoagulated with ethylene diamine tetraacetic acid.

实验评估：造模后4周，5只模型观察大鼠制作脑切片，行苏木精-伊红染色镜检。

ICR fetus mice were sterilely obtained to culture mouse embryonic fibroblasts by tissue pancreatin digestion method.

无菌条件下取出ICR胎鼠，组织块胰酶消化法分离培养小鼠胚胎成纤维细胞，接种在0.1%明胶包被的6孔板中即为饲养层。

METHODS: Fasting venous blood of participants in two groups was harvested sterilely at 6 months and 1 year after test group restoration, serum nickel and chromium content were determined using graphite furnace atomic absorption spectrometry.

无菌抽取对照组健康者及实验组患者修复后0.5年，1年空腹静脉血，采用石墨炉原子吸收光谱法测定血清中镍、铬元素的含量。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。收获的NASC传代培养，用流式细胞仪检测细胞的免疫表型。

Five male Wistar rats of 4－5 weeks old were executed by cutting off the heads to sterilely obtain the muscle group in hind limbs, remove the fat and the fascia, so as to prepare for single cell suspension.

选取4～5周龄Wistar雄性大鼠5只，脱颈处死无菌切取后腿肌群，剪除脂肪和筋膜，制备单细胞悬液。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum. The acquired NASCs were subcultured for passage.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。

METHODS: Mouse bilateral femur was sterilely isolated. BMSCs were harvested by the Percoll density gradient centrifugation, and purified and proliferated by the adherent method.

无菌分离小鼠双侧股骨，Percoll密度梯度离心法分离骨髓间充质干细胞，再用贴壁筛选法纯化扩增。

On basis of mention , we investigate the ability of isolation, purification and expansion of adult rat mesenchymal stem cells to nerve cells in vitro.MethodsBone marrow mononuclear cells from adult rat femur and tibia were obtained sterilely by Percoll ( 1. 073 103 g/L ) gradient centrifugation .

根据MSCs与其它细胞的密度不同而采用Percoll(1.073×10~3g／L)分离液将其分离，此方法分离MSCs所获得的细胞种类较单一，Percoll分离液将骨髓细胞分为三层，取含MSCs的低密度层进行培养。

Methods Human umbilical cord blood samples were obtained sterilely from healthy parturients. MSCs were isolated by lymphocyte separation medium (density 1.077g/ml), MSCs were obtained by adhere culture. The surface antigen expression of MSCs was detected by flow cytometry.

将无菌条件下采集健康产妇脐带血，用相对密度为1.077g/ml的人淋巴细胞分离液分离脐血单个核细胞，利用间充质干细胞贴壁生长的特性获得MSCs，流式细胞仪检测其表面抗原标志。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。