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The present invention relates to a process for rapid solution synthesis of a peptide, the process comprising repetitive cycles of steps-: a coupling step, using an excess of an activated carboxylic component to acylate an amino component, a quenching step in which a scavenger is used to remove residual activated carboxylic functions, wherein the scavenger may also be used for deprotection of the growing peptide, one or more aqueous extractions and optionally, a separate deprotection step, followed by one or more aqueous extractions, characterised in that the process comprises at least one step, referred to as step, in which an amine or a thiol comprising a free anion or a latent anion is used as a scavenger of residual activated carboxylic functions.

本发明涉及快速溶液合成肽的方法,该方法包括步骤-的重复循环:偶联步骤,使用过量的活化羧基成分酰化氨基成分,淬灭步骤,在该步骤中使用清除剂除去残余的活化羧基官能团,其中该清除剂还可以用于使生长中的肽去保护,一或多个水抽提和任选地,独立的去保护步骤,之后进行一或多次水抽提,其特征在于该方法包括至少一个步骤,称作步骤,在该步骤中包含自由阴离子或潜阴离子的胺或硫醇用作残余活化羧基官能团的清除剂。

During these steps, some digits are selected for specific roles (e.g., first addend) in the addition operation, while the others are held in working memory, but not used in the current operation.

在这些心算步骤中,每个时刻只有一些数字被特殊地选择用于加法运算的被加数,而其他的数字则仅仅保存在工作记忆中并不用于当前操作。

Use the NetShell command to clear, delete or refresh the Address Resolution Protocol cache by following the following steps.

使用的NetShell命令来清除,删除或刷新地址解析协议缓存按照下列步骤。

One of the most important steps that you will need to take when it comes to becoming adept at great parenting is that of controlling your anger.

成为一对好的父母的一个最重要的,也是必经的过程,就是学会控制好你的怒气。

Cell adhesion is one of important steps in the multi-step process of gastric cancer pathogenesis.

细胞黏附是胃癌发病的多阶式机理中的重要一步。

To ensure close adhibition of connecting board, screwing should start from center to outside, that is, screwing from enlarged center to skirting area. To avoid coating's influence on tensile force, screwing work should be finished on one day. And to decrease prestress' difference of high-tension bolt, bolt screwing runs in two steps: primary screwing and final screwing. For large-scale joint with great amount of bolts demands a second screwing.

为使被连接板叠密贴紧,应从螺栓群中央顺序向外施拧,即从节点中刚变大的中央按顺序向下受约束的边缘施拧,为防止高强度螺栓连接副的表面处理涂层发生变化影响拉力,应在当天终拧完毕,为减少先拧后拧的高强度螺栓预拉力的差别,其拧紧必须分为初拧和终拧两步进行,对于大型节点,螺栓数量较多,则需要增加一道复拧工序。

A two-steps numerical method has been used to deal with this process. In every computation time-step, two phases are used: the first one is the adiabatic expansion and cracking phase; the second one is the phase of absorbing laser energy.

采用两步数值计算方法处理等离子体微滴的爆炸过程,即在每一个数值计算时间步长内,将爆炸过程分为两步,第一步处理微滴的绝热膨胀及裂解过程;第二步处理微滴对激光脉冲能量的吸收过程。

Nelder and Mead also resolved the question of what to do if none of the steps tried bring acceptable improvement by adding a shrink step: when all else fails, reduce the lengths of the edges adjacent to the current best vertex by half, as is also illustrated in Fig.

Nelder 和 Mead同时也解决了如果增加一个缩小步长没有一个尝试步长是可接受的改进怎么办的问题:如果都失败,将与当前最好顶点相连的边减半,正如图3所示。

He made no reply to this adjuration; only plodding doggedly down the wooden steps and halting before an apartment which from that halt and the superior quality of its furniture I conjectured to be the best one.

他对于这个恳求没有答理,只是固执地、沉重缓慢地走下木梯,在一间屋子的门口停下来。从他那停步不前和屋里家具的上等质料看来,我猜这是最好的一间了。

As an admin, you need to identify the important steps and points in doing that.

作为管理员,您需要确定的重要步骤,而且在这样做点。

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When I started coding, I had problems with the ASF file writer.

当我开始编码,我曾与ASF文件作家的问题。

I know that in one of the schools the boys have risen up in the classroom and enlisted in a mass.

如果你们也和他们一样,是集体自愿的话,你们的老师将是多么地光荣啊。

But in monopolizing industry, because of limit of industrial policy and financial policy, the development of non-state owned economy has come under serious suppression.

但在垄断性产业上,由于产业政策、金融政策上的限制,民营经济的发展受到了较为严重的抑制。