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Fig 1 Negative control Female umbilical cord sample has no Y-specific signal after in situ hybridization with the biotinylated Y-repeated sequence DNA probe PY3.4 Fig 2 Positive control Flow-sorted male umbilical cord cells hybridized to Y-specific DNA probe PY3.4,Every cell contains a Y-specific signal Fig 3 Fetal cells sorted from maternal blood Flow-sorted cells from a pregnant woman at 20 weeks of gestation hybridized to Y-specific DNA probe PY3.4,containing a Y-specific signal Fig 4 The result of polymerase chain reaction Lane 1:male umbilical cord NRBCs sorted by FITC-conjugated anti-monoclonal glycophorin A;Lane 2:sample of 2;Lane 3:male umbilical cord NRBCs sorted by FITC-conjugated anti-CD36;Lanes 4-5:samples of 1 and 3;Lane 6:50 cells of male;Lane 7:5 cells of male;Lane 8:200ng male DNA(positive+control);Lane 9:nonpregnant female DNA(negative+control);Lane 10:ΦX174 HaeⅢ Maker,271bp:amplified band of Y-specific gene SRY;383bp:amplified band of human β-globin gene
图1 阴性对照女性脐带血标本,经与生物素标记的Y-染色体重复序列DNA探针原位杂交后未见Y-染色体特异信号图2 阳性对照分选的男性脐血细胞经与Y-特异DNA探针杂交后,每一细胞都含有Y-染色体特异信号图3 母体外周血中分选的胎儿细胞从一位妊娠20周的孕妇外周血中分选出的细胞经与Y-特异DNA探针杂交细胞中含有Y-染色体特异信号图4 聚合酶链反应结果 1:GPA-FITC单抗分选男胎脐血NRBCs;2:2号标本;3:CD36-FITC单抗分选男胎脐血NRBCs;4、5:1、3号标本;6:50个男性细胞标本;7:5个男性细胞标本;8:200ng男性DNA;9:未孕女性DNA;10:ΦX174 HaeⅢ标准,271bp:为SRY基因扩增带;383bp:为人β-珠蛋白基因扩增带内参照
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The protocol for the detections of all the AIV isolates, the H5-subtype and the optimization of reactions were studied with a H5-subtype reference isolate BSG1. A 229bp-band of AIV specific and a 380bp-band of H5 subtype specific were amplified individually with the designed primers of AIV specific and H5-subtype specific respectively by the developed RT-PCR technique from isolate BSG1. A double-PCR was also developed which can detect all the AIVs and identify the H5-subtype AIVs. The results of specificity experiment showed that there was no specific band could be amplified with the samples of Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, egg drop syndrome virus and infectious bursal disease virus.
结果应用通用引物可从BSG1毒株扩增到与预计大小相符的229bp AIV特异性片段,应用H5亚型鉴定引物则可从BSG1毒株扩增到与预计大小相符的380bp H5亚型特异性片段;在此基础上建立了可检测所有AIV毒株以及可同时进行H5亚型毒株鉴定的二重RT-PCR方法;检测方法的特异性试验结果表明,该方法对新城疫病毒、传染性支气管炎病毒、传染性喉气管炎病毒、产蛋下降综合症、传染性法氏囊病毒无交叉反应;检测方法的敏感性试验结果表明,通用引物扩增、H5亚型特异性引物扩增以及二重PCR扩增反应的组织样品总RNA的最低检出量分别为0.0217μg、0.0217μg和2.17μg;对20份临床样品检测中,有10份为AIV阳性,其中有5份为H5亚型病毒阳性。
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The narrow sense of POBS refers to the prohibition to the specific person\'s competitive specific behavior who has specific relational with the specific business,and refers to an oblige side is authorized to request a special person who has a specific civil legal relationship with him not to carry on a competition behavior aiming at him.
狭义的竞业禁止指对与特定营业具有特定关系的特定人所为的竞争性特定行为的禁止,是指权利人一方有权要求与其有特定民事法律关系的特定人不得为针对自己的竞争性行为。
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This paper is divided into seven chapters specific research project analysis and control, tender offers analysis and decision-making of specific methods, and cost-plus bids methods, and through analysis of specific cases to find out the cost of the project analysis and the integration of the tender offer specific strategies to identify cost-effective ways of increasing and concrete methods.
本论文分七个章节具体研究工程项目的分析与控制,投标报价的分析决策与具体方法,及成本加利润的投标报价方法,并通过具体案例分析寻找出项目成本分析与投标报价相结合的具体策略,从中找出降低成本增大效益的途径和具体方法。
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With reference to the experience of specific practice on strategic alliance in typical domestic and overseas port like New Jersey Port in New York, port of Kaohsiung, Keelung and Taizhong, major ports in Yangtze River Delta, the possible modes of Rizhao Port strategic alliance were proposed. Based on the previous analysis, specific design on the supply chain management system of Rizhao Port viewing from strategic alliance was given in this dissertation, which mainly covered two aspects. On the one hand, based on the overall review of common design process, structure design and selection of partnership of supply chain management, and with consideration of the development trend of Rizhao Port, the double-sector style supply chain structure mode of Rizhao Port was put forward based on strategic alliance. On the other hand, in order to make it more clear on the specific process and contents of this kind of supply chain structure, the specific implementation of five supply chain for Rizhao Port in which capital was taken as the hub were proposed, named as, the coal supply chain based on norm regular ship transport mode, the ironstone supply chain based on cost optimization, the crude oil transport supply chain with the aim of extending petrifaction industrial chain, the timber transport supply chain based on circulating economy concept, and the farming transport supply chain based on futures theory.
在前文分析的基础上,论文重点论述了基于战略战略联盟的日照港口供应链管理的总体框架构建和具体规划设计两方面:一方面,在对供应链管理一般构建流程、结构设计、供需均衡及合作伙伴选择等方面进行全面总结基础上,并结合日照港口的发展趋势,推出了基于纵向战略联盟的日照港口集团式双扇形供应链结构模型;另一方面,为进一步理清日照港口双扇形供应链结构中相关供应链的具体流程及内容,特提出了以资本为纽带日照港口五大供应链的具体设计,即基于准班轮运输模式的日照港煤炭供应链,基于成本最优化日照港口铁矿石供应链,以延伸石化产业链为目标的日照港原油运输供应链,基于循环经济理念的日照港木材运输供应链,基于期货理论的农业定单式日照港农业运输供应链。
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Based on the model, the metabolic bioreaction rates such as the specific substrate consumption rate, the specific growth rate, the specific acetyl-CoA formation rate as well as the specific oxygen uptake rate are obtained.
利用该模型可以解得比碳源消耗速率、比生长速率、比乙酰辅酶A生成速率、比氧消耗速率等关键的反应速率。
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aeroallergen specific ige antibody in the serum of 625 patients with allergic rhinitis were positive. total ige in serum was positive in 413 cases, accounting for 66.1%. the positive rate of total ige was less than that of aeroallergen specific ige. the positive rate of aeroallergen specific ige in order were mite mixture (84.32%), cockroach (19.04%), cypress (15.20%), cat-dog epithelia (13.12%), tree mixture (7.20%), ragweed (5.12%), humulus lupulus (1.76%), mugwort (1.76%), mould mixture (1.44%). in the two groups, the positive rate of aeroallergen specific ige in order were similar.
625例变应性鼻炎患者中吸入性变应原特异性ige阳性率依次为尘螨84.32%、蟑螂19.04%、柏树15.20%、动物毛皮屑组合13.12%、树木花粉组合7.20%、矮豚草5.12%,葎草1.76%、蒿草1.76%、霉菌组合1.44%;总ige阳性者413例(66.1%)明显低于特异性ige阳性率;两组中最常见的变应原相似;未成年组屋尘螨的阳性率及敏感程度明显高于成年组(p 0.01)。
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Experimental animals exhibited specific antibodies that were detectable 15 days after the first injection and increased steadily, the IgG2a-specific antibody titers of DNA groups were higher than IgG1-specific antibody titers, and the recombinant protein groups were contrary. The DNA vaccines elicited a T-cell-proliferative responses as well as IFN-γ production upon restimulation with the specific antigens using ELISPOT assay; the ratio of CD4+/CD8+ were lower than that of the control mice.
本研究成功构建了布鲁氏菌基因工程疫苗,在国内尚属首次,利用流式细胞仪的分型技术和ELISPOT法评价布鲁氏菌疫苗的细胞免疫指标将促进我国兽用疫苗及相关产品的实验室质控标准方法的建立和发展,为他们的临床应用奠定基础。
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The limitations of two kinds of life tables, time-specific life table and age-specific life table were analysed and resolved. The limitation of time-specific life table could be solved by adding the survival rate of immature stages.In age-specific life table, the durations of each stage and the whole generation could be calculated by a formulary detruded from the life table, and rmcould be got from the generation duration.
针对特定时间生命表和特定年龄生命表两种编制方法中存在的不足进行了分析,并提出了可行的解决办法,即在特定时间生命表中导入幼期存活率,在特定年龄生命表中根据推导出的历期公式计算各个虫龄的历期,得到世代平均历期T,进而得到rm值。
- 相关中文对照歌词
- Father Sky
- One Specific Thing
- Bring It Back
- Stop Lyin'
- Scenster
- Talking Winning
- Invisible Ink
- The General Specific
- The Ruler And The Killer
- Turn Back
- 推荐网络例句
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It is like a mustard seed, which a man took and planted in his garden.
好像一粒芥菜种,有人拿去种在园子里,长大成树,天上的飞鸟宿在他的枝上。
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The increase in narcissism was stronger for women than for men in both datasets.
在自我陶醉的增加是更有力的比这两个数据集男性。
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This human is in bardo with earth ' s counsels at this time.
这个人目前正处于中阴身中,和地球的法律顾问会呆在一起。