查询词典 southern

PCR, Southern hybridization and SOD activity assay displayed that exogenous gene was integrated in five maize genomes, exogenous protein was over-expressed in partial plants and the activity of MnSOD of the transformed plants is more than 3 times of the untransformed control.

PCR、Southern杂交和SOD酶活性电泳检测表明，5个玉米基因组中已整合外源基因，部分转基因植株过量表达了外源蛋白，转基因株系中的MnSOD酶活性是未转基因对照植株的3倍以上。

Molecular analysis and disease-resistance test The regenerated kanamycin resistant plants were analyzed by PCR andPCR-Southern reaction with the untransformed plants as negative control, plasmidDNA as positive control.

转基因植株分子检测及抗病性鉴定以未转化植株为阴性对照，质粒DNA为阳性对照，nptⅡ基因和chit42基因的特异引物进行PCR和PCR-Southern检测。

Southern Vectis, which operates buses on the Isle of Wight, off England's south coast, said it was to contact local language schools following several complaints about the behavior of young students over the summer vacation.

位于英国南海岸怀特岛的Southern Vectis巴士公司称，由于接到几个有关学生暑假期间上车不排队的投诉，公司决定与当地的语言学校取得联系，解决这一问题。

Southern hybridization, Northern hybridization and enzyme activity determination all confirmed the effective expression of sorghum Sorghum vulgare L.

经Southern杂交、Northern杂交和酶活性检测表明，高粱C4型NADP-ME可以在水稻中有效表达，酶活性可被提高1～7倍。

Southern blot analysis showed that there were two copies of the gene in the three accessions.

Southern blot分析结果表明，三种材料中该基因均含有两个拷贝。

METHODS Human IL 6 gene was reconstructed in retrovirus vector and transferred into incasing cells PA317 by lipofectamine mediated method. The clones of the cells transferred with hIL 6 were selected by G418. Targeted NIH3T3 cells were infected with the virus granules secreted from PA317 and also selected by G418. The insertion and expression of hIL 6 gene in NIH3T3 cells were analysed with Southern blot and Northern blot. RESULTS Human IL 6 retrovirus vector (pZIPIL-6) was successfully reconstructed.

利用重组载体构建技术将质粒pUCIL 6 cDNA的目的片段连接于逆转录病毒载体上，并以脂质体介导的方法将重组载体转染包装细胞PA317，以G418筛选克隆细胞，浓缩克隆细胞上清以制备重组病毒液，继之感染NIH3T3细胞后，进行Southern blot和Northern blot分析，检测目的基因在靶细胞的整合与转录水平。

Methods The expression of CD44 mRNA was examined in cancerous tissues and adjacent noncancerous mucosae from 26 patients with colorectal cancer and in colorectal polyps from 7 patients by reverse transcription-polymerase chain reaction and Southern blot hybridization.

应用逆转录、多聚合酶链反应和Southern杂交技术，检测26例大肠癌患者癌组织、正常大肠粘膜组织和7例大肠息肉CD44基因mRNA表达。

The novel PCR technology can accurately categorize the spectrum of FMR1 alleles, including alleles previously considered too large to amplify; reproducibly detect low abundance full mutation alleles; and correctly infer homozygosity in female samples, thus greatly reducing the need for sample reflexing to Southern blotting.

这种新型的PCR技术可以准确判断FMR1基因的类型，包括传统PCR不能扩增的大片段。该法还能检测低浓度的全突变条带，并且重复性好。同时该法还能准确判断女性样本中的纯合子，因此大大减少了需要Southern杂交的样本量。

The genomic DNAs of the transgenic plants carrying Ac:: GUS were probed by Ac DNA in Southern blot analysis. Ac had excised from its original positions in T-DNA and reinserted at other genomic sites.

Ac因子作探针，与上述转基因植株的基因组DNA进行Southern杂交，结果表明，Ac从T-DNA解离后，重新整合到烟草基因组的其它位点。

In this study, 105 TDFs were in silico mapped in the rice high-density linkage map. Nineteen TDFs were mapped to the quantitative trait loci regions for root growth under water-limited conditions in, at least, two of three rice populations derived from three crosses with the same parent of Azucena (Bala×Azucena, IR64×Azucena and IR1552×Azucena). Four of 19 genes (T37, L16, T17 and T7) were mapped based a RIL population of IR1552×Azucena by southern blot analysis. Five genes encode putative or hypothetical protein. Other 14 genes were similar with known genes in databases including expansin (OsEXP2), late embryogenesis-abundant gene , an SR-related protein essential for spliceosome assembly (SART1), autophagocytosis protein , bHLH protein, fruit-ripening protein similar to ASR, nickel-binding protein 2A, DNA-binding protein, pyruvate dehydrogenase kinase , stomatin-like protein, SR1 induced by sucrose starvation, vacuolar protein sorting protein (VSP33a), gibberellin action negative regulator and retroelement.

根据核苷酸序列将105个基因电子定位到水稻的高密度连锁图谱上，其中19个差异表达基因定位在Bala×Azucena、IR64×Azucena和IR1552×Azucena中至少两个群体共同的与根生长相关的QTLs区间，并用Southern杂交将其中的4个定位到IR1552×Azucena群体遗传连锁图谱相应的位点上。19个基因中的5个编码推断的未知功能蛋白质，其余14个编码已知功能蛋白，分别为膨胀素（Os-EXP2）、胚胎后期丰富蛋白、剪接体安装必需的SR相关蛋白（SART1）、自吞噬蛋白、碱性螺旋-环-螺旋转录因子、与ASR相似的果实成熟蛋白、镍结合蛋白、DNA结合蛋白、丙酮酸脱氢酶激酶、stomatins类蛋白、蔗糖调节蛋白SR1、液泡蛋白分类蛋白（VSP33a）、GA负调节因子、逆转座元件。

On closer examination, though, this is not a vote for multilateralism but just the opposite.

仔细审视后我们发现，这并非是对多边主义投出的赞成票，而是恰好相反。

Uncovering their weak spots, so I can defeat them.

揭露出他们的弱点，这样我就可以打败他们了。