查询词典 sera

Serum samples from cases with schistosomiasis or other helminth infections were tested. Results The positive rate of MPAIA was 96.7%（116/120） with the sera of S. japonicum -infected cases. No cross reaction was observed with sera of trichinellosis, paragonimiasis or cysticercosis cases.

结果 用磁微粒分离酶联免疫法检测日本血吸虫虫卵抗体，阳性检出率为96.7%（116/120），与旋毛虫、并殖吸虫、嚢尾蚴等其他寄生蠕虫抗体无交叉反应现象，检测试剂4 ℃可保存12个月。

There are high level of specific IgG and IgA against Fe-SOD in sera and lavage of intestines in mice intranasally administrated with S. typhi Fe-SOD, but there are high level of specific IgG in sera only in mice immunized with S. typhi Fe-SOD peritoneally.

结果 当用IL-1作为佐剂时，经鼻腔接种伤寒杆菌Fe-SOD的小鼠血液和肠液中可产生高水平特异性IgG和IgA；而腹腔注射仅在血液中产生高水平特异性IgG。

Methods The present study included twelve undialyzed ESRD patients with anemia.The sera from the uremic patients were added to CFU-E and BFU-E culture in the concentrations ranging from 1.25% to 5%.In vitro CFU-E and BFU-E growth in the presence of sera from ESRD patients was compared with in the presence normal human subjects with the use of normal mice bone marrows.

选择12例尿毒症肾性贫血未透析患者为试验组，另选正常健康体检者12例为正常对照组，将实验组、正常对照组血清分别稀释成不同终浓度，采用甲基纤维素细胞培养的方法对小鼠骨髓细胞进行培养，对比不同浓度血清组红系集落形成单位、红系爆式形成单位的集落数。

For evaluating the vaccinal effect,This indirect ELISA was used to detect sera collected from 160 dogs; after one year, 60dogs among these dogs was also collected sera for detection in this indirect ELISA.

为了评估犬细小病毒的免疫情况，将建立的间接ELISA方法应用于140份犬细小病毒血清抗体的检测，而且一年后，对其中的60只犬再次采集血清进行检测。

Owing to the variability of HVR1, the method to check anti-HVR1 antibodies need to cloning and expressing the gene of HVR1 of every sera and that poses some problem. For example, it needs lots of time and money, and the sample must come from viremic patients. It is urgent to acquire a broadly cross-reactivity HVR1 antigen to check the antiHVR1 Ab in the sera of HCV infected patients by ELISA.

由于HVR1的高变性，因此要想测定血清中抗HVR1水平，通常要克隆表达待测血清中的HVR1基因，但该方法时间长、费用高，并仅对HCV、RNA阳性血清适用，所以急需一种具有极高交叉反应性的HVR1抗原，才可通过ELISA对病人的HVR1抗体进行检测。

Results In 30 sera with anti-Toxoplasma IgM antibodies and 28 with IgG antibodies, the sensitivity for IgM and IgG antibody detection was 90.0%（27/30） and 85.7%（24/28） respectively, the specificity was all 100% in examining 40 healthy control sera, and the Youden index was 0.9 and 0.86 respectively.

结果 用免疫印迹试剂盒检测30份弓形虫IgM和28份IgG阳性血清，敏感性分别为90.0%（27/30）和85.7%（24/28），40份健康对照者血清的弓形虫IgM和IgG抗体均为阴性，特异性均为100%，Youden指数分别为0.9和0.86。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

To exam Kala - azar patients, sera and healthy persons, sera from epidemic area.

目的用从新疆不同地区分离得到的利什曼原虫前鞭毛体提取抗原，观察ELISA法检测黑热病病人和疫区健康人血清抗体时的敏感性和特异性，为制备ELISA诊断试剂盒和进一步提高这种检测方法的敏感性和特异性，提高黑热病现场流行病学调查结果的准确性和可靠性提供实验依据。

Under the Precondition of relative standard Salmonella typing and phase induction, home-made diagnostic sera combined with Thailand diagnostic sera may be applied in primary laboratory to accomplish an efficient and cost-effective serotyping.

建议基层实验室在掌握正确的使用习惯和建立相对标准化的沙门菌分型及相位诱导方法的前提下，使用国产血清搭配部分泰国因子血清的组合，即能达到低成本和高效率的分型效果。

12O Tibete foi,é e sempre será uma parte da China!13 Tíbet fue, es y siempre será parte de China!

我们不会放弃每一寸国土，即便他还很落后。

He became prime minister in 1997 and won international acclaim for his role in the Northern Ireland peace process.

他在1997年成为总理，并且因为在北爱尔兰的和平进程中发挥的作用受到国际社会的赞许。

Mixed Page Sorting Method Using the cuttable user model, this thesis proposes a mixed page sorting method by using cutted user model in server-side and complete user model in client-side.

隐私分级利用的混合式页面排序方法针对个性化搜索中搜索结果排序的问题，本文利用隐私可控开放的用户偏好模型，提出一个在客户端和服务器端分步进行的隐私分级利用的混合式页面排序方法。