查询词典 sequential estimation

In this study, we performed sequential combined PI and DAPI staining and FISH with two different 45S rDNA clones on meiotic pachytene and mitotic metaphase chromosomes in tomato.

采用CPD染色对番茄减数分裂粗线期和有丝分裂中期染色体进行了显带分析，随后用两种不同的45S rDNA 克隆在相同的分裂相进行了荧光原位杂交定位分析。

The system includes a sequential manual mode: by moving the shift level to the M gate, the driver can choose to select gears using the shift lever or paddle shifts on the steering wheel.

该系统包括一个顺序手动模式：通过移动转移水平的M门，司机可以选择使用选择齿轮换档杆或桨方向盘上的变化。

The engine is connected to either a seven-speed manual gearbox or a seven-speed sequential automatic transmission which is available with or without steering wheel mounted paddle shifters.

发动机是连接到一个七速手动变速箱或七速顺序自动变速器，可有或没有安装方向盘换档。

No, Pairwise does not use sequential elimination.

没有，成对不使用顺序消除。

Sequential and parallel ports, interface for floppy- drive and PS/2 can be realized optionally.

时序和并口，接口软传动及PS/2能实现可选择。

Passive power filters is adopt widely because it is simple configuration, convenient maintenance, low cost and etc. Otherwise, passive power filters is easy affect by change of system parameters, can' t remove sequential harmonics, and easy arose series and parallel resonance with system.

无源电力滤波器由于其结构简单，维护方便，造价低廉等优点仍然成为目前普遍采用的一种滤波手段，但是无源电力滤波器受到系统结构变化影响较大，不能消除连续谐波，且容易和系统阻抗发生相互作用，引起串联、并联谐振，严重时甚至使系统崩溃。

Sequential spline function fast algorithm and a dimension-reducing algorithm for the parametric variable spline for data processing are deduced, whose advantage in memory and computing speed is discussed.

推导了数据处理的序贯样条快速算法及参变量样条凝聚算法，讨论了它们在存储量和运算速度方面的优点。

Target cells in bone for parathormone and calcitonin are different: enrichment for each cell type by sequential digestion of mouse calvaria and selective adhesion to polymeric surfaces［J］.

改良组织块混合酶消化法成骨样细胞培养［J］。中国骨伤，2003，16(11)：661-663。［6］高飞，霍建忠。不同龄小鼠成骨细胞OPG和RANKL表达的变化［J］。

In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

为了研制副结核病敏感、特异的诊断试剂和新型、高效的预防制剂，尤其是DNA疫苗，本研究筛选了M.paratuberculosis主要保护性抗原SOD基因，以M.paratuberculosis C-2染色体DNA为模板，以SOD基因的特异性引物进行PCR扩增，获得了624bp的SOD基因，通过T-A克隆技术，将PCR产物克隆至pMD18-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pMD18-T-SOD，序列测定及DNASTAR分析表明，所获得的M.paratuberculosis C-2 SOD基因与Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致，两者核苷酸序列的同源性为99％，氨基酸序列的同源性为99.5％，表明该基因在副结核分枝杆菌中是高度保守的。

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能，本研究选择了MAP的主要保护性抗原Hsp65蛋白，以副结核分枝杆菌C-2株染色体DNA为模板，以hsp65基因的特异性引物进行PCR扩增，获得了1 626bp的hsp65基因，通过T-A克隆技术，将PCR产物克隆至pGEM-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pGEM-T-hsp65，以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列，结果表明，所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致，两者核苷酸序列的同源性为99.1％，氨基酸序列的同源性为99.3％，表明该基因在副结核分枝杆菌中高度保守。

Don not attempt to do something which you can not to do.

不要企图做那些办不到的事情。

The expression of CTGF and TNF-αweredetected by immunochemistry and the number of Clara Cells was calculated.

光镜下观察肺组织的病理变化，采用免疫组化染色观察肺组织中结缔组织生长因子和肿瘤坏死因子-α的表达和Clara细胞的数量。