查询词典 sequencing problem

Direct sequencing of DNA amplified by PCR was used with the primers designed according to the sequence of control region and the neighbor region. Sequences were analyzed and compared base on Sequencing Analysis 3.4 and Seq/Ede software. DP of mtDNA in Yao ethnic group was calculated. MAGA2 software was also employed to analyze the genetic distance between samples and construct the phylogenentic tree.

以南方民族瑶族为研究对象，根据mtDNA控制区及周围区域的序列设计引物，用PCR产物直接测序对105份无关瑶族样本mtDNA控制区进行了测序，通过Sequencing Analysis 3.4和Seq/Ede软件分析比对，得到的序列结果，计算瑶族mtDNA单倍型的识别率，并应用MEGA 2软件对数据做进化距离分析，构建系统发育树。

These specific cDNAs and DNAwere subcloned into vector for sequencing.The sequencing results demonstrated thatthe specific cDNA from P.chinensis eyestalk consists of 203 base pairs,and all thespecific cDNAs from the shrimps T.curvirostris,P.stylirostris and the crab E.sinensis consist of 215 base pairs.

分别将这些片段亚克隆到载体中进行测序，测得中国对虾特异性cDNA片段由203个碱基组成，中华绒螯蟹、鹰爪虾、蓝对虾的特异性cDNA片段及蓝对虾的特异性DNA片段由215个碱基组成，其中蓝对虾的特异性cDNA与由蓝对虾的基因组DNA扩增得到的特异性DNA片段的碱基序列几乎完全相同。

RESULTS suc2 gene fragment of about 1.5 kb was amplified and cloned. DNA sequencing confirmed that , compared with the published sequence, the cloned suc2 gene had correct sequ ence except for one point mutation, but that did not alter the amino acid sequence o f t he encoded peptide. By transformation of yeast cells with a constructed gene dis ruption vector, four yeast clones were obtained which showed correct auxotrophy. PCR and DNA sequencing indicated that these yeast clones had the expected genot ype.

结果用 PCR从酵母基因组DNA中扩增出大小约1.5 kb的suc2基因片段，序列测定表明除585位有一点突变外，所得suc2基因与文献报道相同，构建的定位突变载体导入酵母细胞后，得到4株营养型符合的突变体，PCR表明这些突变体中均有suc2基因的同源重组，取其中1株的PCR产物进行序列测定证实了同源重组的发生。

In the process of the rapid sequencing and merging the two sorting algorithms are correct for the test, with the manual input of data, the procedure is basically completed the data entry for the rapid sequencing and merging order!

在对本程序的快速排序和归并排序这两种算法的正确与否进行验证时，用手动输入的数据，本程序基本上完成了对输入数据进行的快速排序和归并排序！

This innovative DNA-sequencing technology was invented by Dr. Jingyue Ju, professor of Chemical Engineering and head of DNA Sequencing and Chemical Biology at the Judith P. Sulzberger, M.D.

这项革命性的DNA测序技术是由化学工程教授Jingyue Ju博士和哥伦比亚大学哥伦比亚基因组中心DNA测序和生物化学系主任Judith P。

The sequencing of design schemes was acquired through sequencing theory of fuzzy numbers.

根据模糊数排序理论对设计方案进行排序并得到最优设计方案。

Because of no signal from N-terminal protein sequencing for 43kDa protein directly, a resulting 18kDa peptide from the partial cleavage of 43kDa protein by BrCN had its N-terminal sequences of AFTFKK determined by N-terminal protein sequencing assay. Then, the 43kDa protein was convinced to the enzyme of 3phosphate glycerate kinase (PGK1) by searching YPD.

由于43kDa蛋白直接进行蛋白质氨基端序列测定时没有信号，因而用溴化氰部分化学裂解43kDa蛋白，得到的18 kDa多肽经蛋白质氨基端序列测定，得到其氨基端前6个氨基酸残基序列为AFTFKK，通过计算机检索YPD，确定43 kDa蛋白是3—磷酸甘油酸激酶（PGK1）。

Methods: total rna was isolated from hepatocellular carcinoma cell line hepg2. subsequently, a 1kb fragment of hccr 2 encoding region was amplified by rt pcr and cloned into promega teasy vector. after confirmed by dna sequencing, the full length encoding region of hccr 2 was amplified by pcr and cloned into pires2 egfp. the recombinant eukaryotic expression vector was confimed by dna sequencing.

从人肝癌细胞株hepg2提取rna，利用rt pcr方法，先克隆出一包括hccr 2编码区的长约1 kb的片段，并将其与t载体连接并转化，测序证实无误后，以hccr 2/t载体为模板，再通过pcr克隆出hccr 2的编码区序列，将其连接到pires2 egfp真核表达载体，并通过测序获得证实。

METHODS: Total RNA was isolated from hepatocellular carcinoma cell line HepG2. Subsequently, a 1kb fragment of HCCR2 encoding region was amplified by RTPCR and cloned into Promega TEasy vector. After confirmed by DNA sequencing, the fulllength encoding region of HCCR2 was amplified by PCR and cloned into pIRES2EGFP. The recombinant eukaryotic expression vector was confimed by DNA sequencing.

从人肝癌细胞株HepG2提取RNA，利用RTPCR方法，先克隆出一包括HCCR2编码区的长约1 kb的片段，并将其与T载体连接并转化，测序证实无误后，以HCCR2/T载体为模板，再通过PCR克隆出HCCR2的编码区序列，将其连接到pIRES2EGFP真核表达载体，并通过测序获得证实。

The universal primers of ITS1 and ITS4 for fungi were used in amplification of genomic rDNA sequences. A target fragment was obtained by ITS-PCR. Then, the fragment is cloned into the vector of pMD18-T before sequencing. The result of sequencing showed that the fragment is 692 bp in length. The results of Blast in Genbank and the analysis of fungal rDNA systemic development show that the relation between this fragment to the fungi of Sporisorium is closest, while the relation between it to the fungi of Ustilago is much far. It suggests that the name of Ustilago scitaminea for sugarcane smut isn't consistent to the result of rDNA systemic development analysis. It still needs more experiments to support this hypothesis.

用真菌rDNA序列分析的通用引物ITS1和ITS4，通过ITS-PCR得到目的片段，克隆在pMD18-T载体上后进行测序，结果显示该片段的长度为692 bp，与Genebank中已报道的真菌序列进行Blast和系统发育树分析，表明所获得的序列与Sporisorium属真菌具有更高的亲缘关系，而与Ustilago属真菌的亲缘关系较远，这与一直沿用的甘蔗黑穗病菌的命名似乎并不吻合，有待进行更多的研究以证实。

A backer of an Afghan law that critics say legalizes marital rape has rejected the international outcry as foreign meddling.

饱受批评的一项法律的拥护者说合法化的婚内暴力不会受国际舆论的干涉。

Years ago, a ancient Egyptian fleet went to the Land of punt for seeking a kind of flavor named "myrrh", and aromatic plants which has dense exotic style for the queen Hatshepsut.

3500年前的一个古埃及的舰队到&彭特之地&寻找一种叫没药的香料。