查询词典 sequencing

They include the variation of the degradement velocity of different sample, the inhibition product of PCR, the amount of DNA extraction, quality of DNA and the heteroplasmy of some tissue. Correct sequencing, comparison of the result of forward and reverse primers sequencing should be done to rule out wrong signals, and prevent contamination. In addition to gene sequencing, low grade heteroplasmy could be dealt by Denaturing high performance liquid chromatography. Multiplex Ligation-dependent Probe Amplification. The analysis of mitochondrial HV Ⅰ, HV Ⅱ sequencing is able to identify the maternal sanguinity, then it could assist in the human identification. It also complements the genomic STR system in distribution of population, the origin of human, and analysis for fossil.

分析粒线体DNA时必须考虑各类检体腐败DNA降解速度之不同、PCR的抑制物质、DNA的萃取量及品质、特定组织的异质现象、萤光序列分析图及正、反向引子定序结果比对、排除杂讯干扰、防治污染等，除以基因定序外，低程度的异质现象也可用Denaturing high performance liquid chromatography、Multiplex Ligation-dependent Probe Amplification等方法分析，粒线体HV Ⅰ、HV Ⅱ碱基序列的分析可鉴别一个人的母系血缘关系、辅助人类身分鉴定，对於族群分布、人类起源探讨、远古检体的分析可作为辅助STR系统的利器。

Specific applications include de novo sequencing and re-sequencing of genomes, metagenomics, RNA analysis, and targeted sequencing of DNA regions of interest. The hallmarks of 454 Sequencing? are its simple, unbiased sample preparation and long, highly accurate sequence reads, including paired reads.

具体的应用包括基因组的从头测序和重测序、宏基因组学、RNA分析和DNA目标区域的定向测序。454测序系统的特点是样品制备简单且无偏向性，读长超长且准确度高。454测序系统的技术已经催生了各个研究领域的几百项经同行评议的研究文章，例如癌症和传染病研究、药物筛选、海洋生物学、人类学、古生物学等各方面研究。

Through grey relation estimation matrix, the threat sequencing is transformed into a MADM problem. The algorithm for the weight of each threat criteria is proposed by information entropy to reduce the effect of the extreme numerical value. Applied the grey related projection value, the treat sequencing is carried out from the aspect of vector projection. In addition, the effects of the identification coefficient and the criteria weights on threat sequencing are investigated respectively.

首先通过建立灰色关联判断矩阵将威胁排序转化为多指标决策问题；其次为缩小极端值的影响提出了基于信息熵的威胁指标权重算法；最后从矢量投影角度应用灰色关联投影值进行威胁排序；并分别研究了分辨系数和指标权重对威胁排序的影响。

The central struggle was between the publicly funded international Human Genome Project, led by Francis Collins, a distinguished scientist and devout Christian, which pursued a slow-and-steady approach to sequencing until Celera, a private company led by Craig Venter, appeared on the scene and boldly claimed that its revolutionary new technique, whole-genome shot-gun sequencing, could do the job in less than half the time.

集中的斗争是与公共基金资助的国际人类整组基因工程有关系的，这个工程是由一个叫Francis Collins领导的，他既是一位卓越的科学家，也是一位虔诚的基督教徒。一个由 Craig Venter领导的私人公司 Celera出现在人们的视野里，并且突如其来地宣称该公司的革命性新技术——整体整组基因散射排序，能够事半功倍；在此之前，国际人类整组基因工程一味追求慢而稳的方法来排序。

Direct sequencing of DNA amplified by PCR was used with the primers designed according to the sequence of control region and the neighbor region. Sequences were analyzed and compared base on Sequencing Analysis 3.4 and Seq/Ede software. DP of mtDNA in Yao ethnic group was calculated. MAGA2 software was also employed to analyze the genetic distance between samples and construct the phylogenentic tree.

以南方民族瑶族为研究对象，根据mtDNA控制区及周围区域的序列设计引物，用PCR产物直接测序对105份无关瑶族样本mtDNA控制区进行了测序，通过Sequencing Analysis 3.4和Seq/Ede软件分析比对，得到的序列结果，计算瑶族mtDNA单倍型的识别率，并应用MEGA 2软件对数据做进化距离分析，构建系统发育树。

These specific cDNAs and DNAwere subcloned into vector for sequencing.The sequencing results demonstrated thatthe specific cDNA from P.chinensis eyestalk consists of 203 base pairs,and all thespecific cDNAs from the shrimps T.curvirostris,P.stylirostris and the crab E.sinensis consist of 215 base pairs.

分别将这些片段亚克隆到载体中进行测序，测得中国对虾特异性cDNA片段由203个碱基组成，中华绒螯蟹、鹰爪虾、蓝对虾的特异性cDNA片段及蓝对虾的特异性DNA片段由215个碱基组成，其中蓝对虾的特异性cDNA与由蓝对虾的基因组DNA扩增得到的特异性DNA片段的碱基序列几乎完全相同。

RESULTS suc2 gene fragment of about 1.5 kb was amplified and cloned. DNA sequencing confirmed that , compared with the published sequence, the cloned suc2 gene had correct sequ ence except for one point mutation, but that did not alter the amino acid sequence o f t he encoded peptide. By transformation of yeast cells with a constructed gene dis ruption vector, four yeast clones were obtained which showed correct auxotrophy. PCR and DNA sequencing indicated that these yeast clones had the expected genot ype.

结果用 PCR从酵母基因组DNA中扩增出大小约1.5 kb的suc2基因片段，序列测定表明除585位有一点突变外，所得suc2基因与文献报道相同，构建的定位突变载体导入酵母细胞后，得到4株营养型符合的突变体，PCR表明这些突变体中均有suc2基因的同源重组，取其中1株的PCR产物进行序列测定证实了同源重组的发生。

In the process of the rapid sequencing and merging the two sorting algorithms are correct for the test, with the manual input of data, the procedure is basically completed the data entry for the rapid sequencing and merging order!

在对本程序的快速排序和归并排序这两种算法的正确与否进行验证时，用手动输入的数据，本程序基本上完成了对输入数据进行的快速排序和归并排序！

The dynamic aircraft sequencing problem in the terminal area was discussed and a mathematical model of aircraft sequencing in the terminal area was established.

讨论了终端区飞机动态排序问题，并利用进化算法建立了终端区航班排序的数学模型。

Proposing a good research thought to resolve the scientific optimizing sequencing problem on air traffic control system based on fuzzy Petri net sequencing algorithm.

算法简单易行，优化效率高，更适合实际的要求，解决空中交通管制系统的科学优化排序问题提供了较好的研究思路。

Some of them were ligated to the plasmid vector pCR 2.1 andsequenced,respectively.

将所获DNA片段克隆到pCR2.1载体进行测序，并与基因组数据库进行同源性比较。

Thinking since Google owns Blogger, I would be able to escalate this issue to the support team.

自谷歌的思考拥有Blogger的，我将能够缓解这一问题的支持团队。