查询词典 sequencing

The aircraft sequencing problem in the terminal area was discussed, a mathematical model of aircraft sequencing in the terminal area was established according to the aircraft wake separation.

讨论了终端区飞机排序问题，根据飞机尾流间隔要求，利用A算法建立了终端区航班排序的数学模型。

This innovative DNA-sequencing technology was invented by Dr. Jingyue Ju, professor of Chemical Engineering and head of DNA Sequencing and Chemical Biology at the Judith P. Sulzberger, M.D.

这项革命性的DNA测序技术是由化学工程教授Jingyue Ju博士和哥伦比亚大学哥伦比亚基因组中心DNA测序和生物化学系主任Judith P。

The sequencing of design schemes was acquired through sequencing theory of fuzzy numbers.

根据模糊数排序理论对设计方案进行排序并得到最优设计方案。

Because of no signal from N-terminal protein sequencing for 43kDa protein directly, a resulting 18kDa peptide from the partial cleavage of 43kDa protein by BrCN had its N-terminal sequences of AFTFKK determined by N-terminal protein sequencing assay. Then, the 43kDa protein was convinced to the enzyme of 3phosphate glycerate kinase (PGK1) by searching YPD.

由于43kDa蛋白直接进行蛋白质氨基端序列测定时没有信号，因而用溴化氰部分化学裂解43kDa蛋白，得到的18 kDa多肽经蛋白质氨基端序列测定，得到其氨基端前6个氨基酸残基序列为AFTFKK，通过计算机检索YPD，确定43 kDa蛋白是3—磷酸甘油酸激酶（PGK1）。

Methods: total rna was isolated from hepatocellular carcinoma cell line hepg2. subsequently, a 1kb fragment of hccr 2 encoding region was amplified by rt pcr and cloned into promega teasy vector. after confirmed by dna sequencing, the full length encoding region of hccr 2 was amplified by pcr and cloned into pires2 egfp. the recombinant eukaryotic expression vector was confimed by dna sequencing.

从人肝癌细胞株hepg2提取rna，利用rt pcr方法，先克隆出一包括hccr 2编码区的长约1 kb的片段，并将其与t载体连接并转化，测序证实无误后，以hccr 2/t载体为模板，再通过pcr克隆出hccr 2的编码区序列，将其连接到pires2 egfp真核表达载体，并通过测序获得证实。

METHODS: Total RNA was isolated from hepatocellular carcinoma cell line HepG2. Subsequently, a 1kb fragment of HCCR2 encoding region was amplified by RTPCR and cloned into Promega TEasy vector. After confirmed by DNA sequencing, the fulllength encoding region of HCCR2 was amplified by PCR and cloned into pIRES2EGFP. The recombinant eukaryotic expression vector was confimed by DNA sequencing.

从人肝癌细胞株HepG2提取RNA，利用RTPCR方法，先克隆出一包括HCCR2编码区的长约1 kb的片段，并将其与T载体连接并转化，测序证实无误后，以HCCR2/T载体为模板，再通过PCR克隆出HCCR2的编码区序列，将其连接到pIRES2EGFP真核表达载体，并通过测序获得证实。

The universal primers of ITS1 and ITS4 for fungi were used in amplification of genomic rDNA sequences. A target fragment was obtained by ITS-PCR. Then, the fragment is cloned into the vector of pMD18-T before sequencing. The result of sequencing showed that the fragment is 692 bp in length. The results of Blast in Genbank and the analysis of fungal rDNA systemic development show that the relation between this fragment to the fungi of Sporisorium is closest, while the relation between it to the fungi of Ustilago is much far. It suggests that the name of Ustilago scitaminea for sugarcane smut isn't consistent to the result of rDNA systemic development analysis. It still needs more experiments to support this hypothesis.

用真菌rDNA序列分析的通用引物ITS1和ITS4，通过ITS-PCR得到目的片段，克隆在pMD18-T载体上后进行测序，结果显示该片段的长度为692 bp，与Genebank中已报道的真菌序列进行Blast和系统发育树分析，表明所获得的序列与Sporisorium属真菌具有更高的亲缘关系，而与Ustilago属真菌的亲缘关系较远，这与一直沿用的甘蔗黑穗病菌的命名似乎并不吻合，有待进行更多的研究以证实。

The central struggle was between the publicly funded international Human Genome Project, led by Francis Collins, a distinguished scientist and devout Christian, which pursued a slow-and-steady approach to sequencing until Celera, a private company led by Craig Venter, appeared on the scene and boldly claimed that its revolutionary new technique, whole-genome shot-gun sequencing, could do the job in less than half the time.

主要竞争存在于公众基金的国际人类基因组项目及Celera公司之间。前者由弗朗西斯。克林斯领导，克林斯本人是位杰出的科学家和虔诚的基督徒。它一开始是寻求一种慢而稳定的方法来测序，但是这种情况在Celera公司出现之后就改变了。Celera公司在Craig Venter的领导下，大胆地主张它的革命性的新技术&whole-genome shot-gun&测序法，该法可以节省一多半的时间。

Dna sequencing of the representative pcr products of carboxyl-terminus of lmp1 gene was analyzed by using four-colored fluorescence terminator sequencing technique.

具有代表性的lmp1基因羧基末端pcr产物采用四色荧光终止序列技术进行dna序列分析。

By mitochondrial DNA control region sequencing the relationship of haplotype phylogenies can be reconstructed to distinguish the Taiwan ring-necked pheasant and imported ring-necked pheasant. Additionally, the sequencing can be used to analyze genetic diversity and genetic structure. The results are expected to provide the reference base for identifying and conserving Taiwan ring-necked pheasant.

本论文研究结论建议，未来进行原生环颈雉族群保育或人工复育计画时，可利用外部形质测量分析、粒线体DNA基因型与遗传多样性分析，做为环颈雉各原生或外来族群的个体辨识工具，并提供拟订族群保育及复育经营措施之参考依据。

Don not attempt to do something which you can not to do.

不要企图做那些办不到的事情。

The expression of CTGF and TNF-αweredetected by immunochemistry and the number of Clara Cells was calculated.

光镜下观察肺组织的病理变化，采用免疫组化染色观察肺组织中结缔组织生长因子和肿瘤坏死因子-α的表达和Clara细胞的数量。