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The nucleotide sequences of the PAP gene and PAPⅡ gene in the root of Phytolacca americana were determined by reverse transcription polymerase chain reaction and PCR. The gene comprises 942bp and 933bp respectively, encoding 313 amino acids and 310 amino acids. When PAP gene and PAPⅡ gene sequences in the root were compared with those in the spring leaves and summer ones, the highest similarity 100% and 99. 8% were observed respectively. They both had one open reading frame . Analysis of the two sequences showed, in fact, they belonged to one species.

以美洲商陆根为材料,提取其总RNA、反转录,用一对PAP基因的特异性引物和一对PAPⅡ的特异性引物,分别对反转录产物进行PCR扩增,将扩增的目的片段进行序列分析,结果扩增出来的PAP基因与已经报道的序列完全相同,扩增出来的PAPⅡ基因与已经报道的序列同源性也在99.8%,而且它们都各自编码一个开放阅读框架,可以说美洲商陆根中肯定存在PAP和PAPⅡ基因,并已经转录。

The nucleotide sequences of the PAP gene and PAPII gene in the root of Phytolacca americana were determined by reverse transcription polymerase chain reaction and PCR. The gene comprises 942bp and 933bp respectively, encoding 313 amino acids and 310 amino acids. When PAP gene and PAPII gene sequences in the root were compared with those in the spring leaves and summer ones, the highest similarity 100% and 99.8% were observed respectively. They both had one open reading frame. Analysis of the two sequences showed, in fact, they belonged to one species.

以美洲商陆根为材料,提取其总RNA、反转录,用一对PAP基因的特异性引物和一对PAPⅡ的特异性引物,分别对反转录产物进行PCR扩增,将扩增的目的片段进行序列分析,结果扩增出来的PAP基因与已经报道的序列完全相同,扩增出来的PAPⅡ基因与已经报道的序列同源性也在99.8%,而且它们都各自编码一个开放阅读框架,可以说美洲商陆根中肯定存在PAP和PAPⅡ基因,并已经转录。

All homologous partial sequences of COI and 16S in Portunidae were collected from GenBank and aligned with the homologous sequences of P.trituberculatus. The NJ tree and MP tree were constructed using Kimura 2-parameter model. Homologous sequences (429 bp) of sampled specimen and genus Charybdis were aligned.

结合GenBank中收集的梭子蟹科COI,16 S rRNA两个基因所有同源序列信息,使用Kimura双参数模型采用邻接法、最大简约法构建梭子蟹科分子系统发育树;将样品蟹测得的COI基因序列和已知属的其他蟹同源序列(429 bp)进行比对分析。

The main results are as follows:(1) In Chapter 2, the proof of the main theorem on quadratically related m-geometric sequences in [5] is simplified and consummated; A family of GMW-geometric sequences derived from GMW sequences are constructed, and their correlation functions and linear complexities are given.

本文也正是从扩频码序列的相关性能和安全性的角度来构造和研究了几类扩频码序列集合,得到以下的结论:(1)第二章首先简化并且完善了文献[5]中关于二次相关m-几何序列的主要定理;其次,在GMW序列的基础上构造了GMW-几何序列,计算了GMW-几何序列的相关函数;最后给出了GMW-几何序列的线性复杂度。

The authors took the Maocaojie section as a typical Permian section, which includes 13 grade 3 sequences that can be divided into two groups: the early craton basin sequences and the late interpolate rifted basin sequences.

以建始县茅草街二叠系剖面为典型研究剖面,将研究区二叠系划分为13个Ⅲ级层序,可分为早期克拉通盆地层序和晚期板内裂陷盆地层序两种类型。

This thesis explains why non-GDJ Golay sequences arise. We found that non-GDJ Golay sequences can be constructed by concatenating or interleaving from a Golay complement pair with two sequences in different Golay cosets.

本论文解释了这些Non-GDJ格雷序列所形成的原因,发现这些Non-GDJ格雷序列是透过Golay所提出的Recursive Constructions,由两组长度为8、四进位跨格雷共集的GDJ格雷互补对所连接出来的。

Sequences were aligned by BLAST method. Results showed that 309bp fragments shared high similarity with orfl38 in Ogu CMS radish and Brassica cybrids of Ogu CMS radish, which 172 nucleotide sequences and 58 amino acids were same among them. And 689bp fragments shared high similarity (100%) with Pol orf224 in B.napus, which 677 nucleotide sequences and 225 amino acids were same among them.

同源性分析结果表明,利用orf138引物所获得的309bP大白菜mtDNA特异片段均与萝卜Ogu CMS、甘蓝型油菜Ogu CMS萝卜体细胞杂种所具有的Ogu orf138高度同源,二者有172个核苷酸完全相同,有58个氨基酸完全相同;orf224引物所获得的689bP大白菜mtDNA特异片段与甘蓝型油菜的Pol orf224高度同源,二者有677个核苷酸完全相同,有225个氨基酸完全相同,同源性均达到100%。

Results Fifty-one clones screened from 160 clones on the basis of Hae III digestion patterns were sequenced, and their sequences were deposited in the GenBank. Clone sequences (52.9%) belonged to Acidimicrobidae and 5 suborders of Actinobacteridae. The other clone sequences (47.1%), which formed one large distinct clade in phylogenetic tree among phylum Actinobacteria, may represent one new suborder or new class.

所获得的51个克隆序列属于39个OTUs,其中52.9%的克隆序列分布于放线菌门放线菌亚的5个亚目和酸微菌亚纲中,并且在这两个亚纲中有大量克隆序列属于放线菌的新类群,另外47.1%的克隆序列以极高的自展值在放线菌门内支持形成一个独立的大分支,极有可能代表一个新亚目或更高级分类单元的类群。

The invention discloses a multi-cell pilot frequency distribution method applied in an orthogonal frequency-division multiplexing system, which comprises the following steps: generating at least two different original frequency hopping sequences according to a predetermined pilot frequency domain; generating a combined frequency hopping sequence (having length greater than that of the original frequency hopping sequences) from at least two different original frequency hopping sequences; generating different pilot frequency structures satisfying collision threshold requirements based on the combined frequency hopping sequence; and distributing the generated pilot frequency structures to each cell.

本发明公开了一种多小区导频分配的方法,用于正交频分复用系统,按照预定的导频频域间隔生成至少两种不同的原始跳频序列;将至少两种不同的所述原始跳频序列生成组合跳频序列,所述组合跳频序列的长度大于所述原始跳频序列的长度;基于所述组合跳频序列生成满足碰撞门限要求的不同导频结构;将所述生成的导频结构分配给各小区。

Ten of them were new sequences and the other 7 were duplicated sequences. These sequences represented respectively: depeptide ABC transporter periplasmic dipeptide-binding protein, permease protein, et al. Conclusion, Gene fragments specific to MTZ-resistant H.

对以上17个差异片段进行单向测序,根据最高同源性比对结果,分别代表二肽ABC载体渗透酶、二肽ABC载体结合蛋白等10个基因的同源基因片段在耐药株中存在高拷贝数。

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