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The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

In the meantime, we got several achievements during the process:Firstly, a sequence encryption algorithm of real-time video in use of one dimension piece-wise linear chaotic map is carried out. This algorithm makes use of Inter' s SSE2 special dictates and makes nonlinear change of chaotic sequences.As a result, the algorithm not only encrypts faster, but also resists several cipher analysis methods. Secondly, this paper presents a block encryption algorithm of real-time video based on chaotic neural network which makes use of numerical transient chaotic neural network to produce chaotic sequences and uses these sequences to do encryption.

同时，在实现系统的过程中，取得了以下研究成果：(1)提出了一种利用一维的分段线性混沌映射来对实时视频进行序列加密的算法，该算法利用Inter的SSE2专用指令集来实现，并对产生的混沌序列进行了有效的非线性变换，从而使该算法不仅加密速度快，并且能有效的抗多种密码分析；(2)提出了一种利用混沌神经网络对实时视频进行分组加密的算法。

Thus a complete section of stratigraphic sequences from Oligocene to Quarternary is obtained, showing 5 second order sequences and 20 third order sequences.

自 1985年起，中国海洋石油东部公司微体古生物分析鉴定人员对珠江口盆地近 5 0口钻井的有孔虫化石进行了定量统计，为高分辨率生物地层划分与对比提供了丰富的资料。

Locations of transgenes in the recovered plasmids were determined by southern blotting. And then adjacent sequences of transgenes integration sites in three recovered aberrant classes were subcloned and analyzed. Transgene of A-2 was inserted into common carp DNA sequences homologous to the mouse phosphoglycerate kinase-1 gene and those of A-3 and A-6 were inserted into common carp DNA sequences homologous to the human epidermal keratin 14 gene and sequence of common carp β-actin gene, respectively.

通过Southern杂交对转植基因在回收质粒上的位置进行了定位分析，并对三种回收到的变异型转植基因旁侧顺序进行了亚克隆和顺序测定。A-2的转植基因整合到了与小鼠磷酸甘油酸盐激酶-1基因同源的鲤鱼基因组顺序旁侧，A-3的转植基因整合到了与人表皮角蛋白14基因同源的鲤鱼基因组顺序旁侧，A-6的转植基因整合到了普通鲤鱼β-actin基因顺序中。

Throughcalculation, we find that there are remarkable differences between coding and non-coding sequences, and vector from random sequences is closely related to that fromthe coding sequences.

通过计算，我们发现，编码区和非编码区所对应的4维向量有很大不同，并且随机序列与编码区对应的向量较为接近。

In order to get suitable binary sequences for key streams, binary generalized cyclotomic sequences of order eight on the two-prime residue class ring were considered. By means of the polynomial theory over finite fields, minimal polynomials and linear complexity of these sequences were obtained.

为了从剩余类环上的二元广义割圆序列中寻求满足需要的密钥流序列，考虑了双素数积剩余类环Z上的一类二元广义8阶割圆序列，利用有限域理论，给出了该序列在不同情形下的极小多项式，进而得到了它的线性复杂度。

Each of the other third-order sequences can be further divided into three system tracts. Based on the identification and correlation of sequence stratigraphic units, and researches on the history of structural evolution, the thickness of sequences and the paleotopography of the area, we proposed the sequence stratigraphical mode of the studied area, and discussed the relations between the sequence stratigraphic model and the oil-gas potentials and estimated the oil-gas potentials of each of the third-order sequences.

针对研究区目的层段所划层序发育的特点，在层序地层单元划分和对比的基础上，结合构造发育史和地层厚度的变化反映出古地形特征，建立了该区的层序地层学模式，并初步探讨了层序地层学模式与含油气性的关系，对各三级层序的含油性做出了初步预测。

A set of equally long finite sequences is said to be complementary sequences if the sum of autocorrelation functions of the sequences in that set is zero except for a zeroshift term.

补码是一种由多个等长的序列组成的、具有良好的自相关性特性的序列集。补码集中每个序列的自相关函数的和在除了零点外的其他任何点上均为零。

The methods for determination of the complete genome sequences of potyviruses, carlaviruses and potexviruses were also established. The complete genome sequences of 13 viruses(20 isolates) were determined while 10 of them were the first report in the world.Five of them including Lily symptomless virus (NC_005138), Lily mottle virus(NC_005288),Onion yellow dwarf virus (NC_005029), Lily virus X (NC_ 007192) and Shallot yellow stripe virus(NC_007433) were selected as the standard sequences of those viruses by National centery of Biotechnology Information, USA.

建立了马铃薯Y病毒属、麝香石竹潜隐病毒属和马铃薯X病毒属成员的基因组全序列扩增技术，完成了13种不同植物病毒近20个分离物的基因组全序列测定，其中10个病毒的基因组全序列为国际首次报道，百合无症病毒(NC_005138)、百合斑驳病毒(NC_005288)、洋葱黄矮病毒(NC_005029)、百合X病毒(NC_ 007192)和葱黄条病毒(NC_007433)等5条序列被美国国家生物技术信息中心列为相关病毒标准序列的有。

The BLAST programs were used for searching protein and DNA databases for sequence similarities. The BLAST result showed that it was a new sequence and similar to zebra fish DNA sequence and some fish reverse transcriptase. But it has small homology of CYP 19 gene sequences that have been in Genbank.In addition, sequences were amplified by RT-PCR with using degenerate primers that weredesigned against CYP4 sequences.

另外，还根据昆虫CYP4家族的特异性氨基酸序列及P450超家族的共同特征序列，采用另一对简并引物，同样进行有关研究，结果显示：疣荔枝螺不同组织的RNA中都含有一长468bp左右的序列，这同样是一段新序列，与部分物种的CYP4存在一定的相关性，可能在螺体内起一定重要的作用，但具体功能有待进一步研究。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。