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Sequences analysis indicated 26 unique cDNA sequences that encoded putative proteins showing similarities to HL35 antigen U, hlim3, heat shock protein, NADH dehydrogenase subunit 1 and calreticulin of H. longicornis, disulfide isomerase of Ambiyomma variegatum and Ixodes scapularis.

结果表明：在长角血蜱雌蜱唾液腺cDNA表达文库中获得26个长角血蜱免疫相关cDNA序列，其编码蛋白与长角血蜱HL35、黏附素hlim3假定蛋白、热休克蛋白、NADH脱氢酶、钙网蛋白以及杂色花蜱和肩突硬蜱的二硫异构酶等具有同源性。

Therefore, the LsHNXs genes were cloned in this study, and their function was studied by RNAi. The main results were shown as follows:1 The conservative sequences of three LsHNXs genes (LsHNX1, LsHNX2, and LsHNX3), and the 3'-terminal specific sequences of LsHNX2 and LsHNX3, were cloned by RT-PCR and 3'-RACE.2 The plant RNAi vectors were constructed and the transformation of Limonium sinense was done. The RNAi lines of three single-genes and one double-gene were obtained and were propogated rapidly for the molecular and the stress tolerance analysis.3 To be compared with wild type, the Na+ and K+ contents secreted by the leaves of LsNHX1 transgenic plants were remarkably higher.

主要实验结果如下：1采用RT-PCR和3'-RACE方法从补血草中克隆获得LsNHXs基因家族三个成员LsNHX1、LsNHX2和LsNHX3的保守区序列以及LsNHX2和LsNHX3的3'端特异序列。2构建植物基因RNAi沉默表达载体并转化补血草，获得三个单基因和一个双基因的RNAi沉默表达植株，快繁转基因植株供分子鉴定和耐逆分析。3LsNHX1转基因植株叶片表面分泌物中的Na+、K+离子含量比野生型对照显著增加；叶组织内Na+离子含量和K+离子含量均略高，但差异不显著；K+/Na+比没有显著差异。4LsNHX2转基因植株叶片表面分泌物中的Na+离子含量比野生型对照有所下降，但差异不显著；K+离子含量比对照显著降低。

The amino acids sequences alignment confirmed that 145 LRR stucture was highly conserved in all aligned PGIP sequences. The results of phylogenetic analysis showed that BoPGIP1 and PGIP from Brassica napus and Arabidopsis thaliana clustered together firstly, and then came from Gossypium barbadense, Lycopersicon esculentum and Phaseolus vulgaris in turn. The clustering results basically accorded with morphological classification system.

将BoPGIP1氨基酸序列与数据库中的其他植物PGIP进行同源序列比对，构建系统进化树，发现所比对基因都含有LRR结构，在145位点的LRR结构域在所有物种中极其保守；系统树显示，该序列最先与甘蓝型油菜和拟南芥聚类，其次和棉花、番茄和豆科作物的PGIP聚类，基本符合按形态特征分类的亲缘关系。

RESULT ①Two different ITS2 sequences (GenBank accession number: AF416783, AF416784) and D3 sequences (AF416782, AF415594), named as An. minimus A and An. minimus C, were detected in this study. They differed by 5. 8％ in the ITS2 region with 22 fixed nucleotide substitutions and indels, and by 1. 5％ in D3 gene with 5 site mutations. There exists individual variation in the COII gene, but this variation is less than that among other members of Myzomyia Series such as An.

结果 ①分别发现两种不同的ITS2序列（GeneBank登录号：AF416783，AF416784）和D3序列（GeneBank登录号：AF416782，AF425594），与微小按蚊A和C同源。A、C两者ITS2序列22个固定位点存在碱基置换和插入／缺失，序列差异为4.8％；D3基因存在5个固定点突变，突变率1.5％。mtDNA-COII基因存在个体差异，但远小于同系其它成员如乌头按蚊、杰普尔按蚊间的变异。

Coding and non-coding sequences are expected to have very different patterns of sequence changes because of the following characteristics of coding sequences: biased codon composition, higher conservation, absence of stop codons and frameshifts, higher fraction of synonymous than nonsynonymous substitutions, higher frequency of substitutions corresponding to changes of chemically similar amino acids, higher proportion of mutable contexts.

我们可以预期可转录的序列及不可转录的序列会有相当不同的序列改变特徵，因为可转录的序列有以下特色：偏颇的密码子组成，较高的保存度，有终止密码子和移码的出现，相较於异义取代有较高比例的同义取代，较高的频率出现化学结构相似的胺基酸取代，较高比例的可变之前后序列。

The sequences of internal transcribed spacer regions of nuclear ribosomal DNA of 9 samples of Nymphaea and related water lilies as outgroups were sequenced and assembled with the sequences in GenBank to analyze the specific and infraspecific relationships.

本文测定了睡莲属9个样品的核核糖体DNA ITS区序列，结合GenBank中获得睡莲类ITS序列，分析了这些样品间的亲缘关系。

Two independent molecular sources were used to reconstruct phylogeny: the 16S rRNA gene on the mitochondrial genome and the 28S rRNA gene on the nuclear genome. A comparison of the sequences showed that the obtained 28S rDNA sequences have evolved at a much slower rate than the 16S rDNA, and that the former is better than the latter for resolving deep branching in the Odonata.

两种独立的分子资源用于重建系统发育发展史：线粒体基因组的16s rRNA基因和核基因组的28s rRNA序列比对表明：获得的28s rDNA序列以远低于16s rDNA的速率进化，并且28s rDNA比16s rDNA更适于分析蜻蜓目的深度分支。

Based on the analyses of the obtained sequences and the sequences from other three representative species of Oscillatoriaceae, and two species of Microcystis , the evolutionary affiliation among the genera of Oscillatoriaceae was discussed.

并通过计算机联网从国际分子生物学数据弹库中获取颤藻科其它种的rDNA基因间隔区序列，通过比较分析，从分子水平对颤藻科Oscilatoriaceae属间的某些分类学问题进行了讨论，并根据序列中核苷酸差异值探讨了颤藻科属间界定的分子标准。

Based on sequence stratigraphy theory and method,and the comprehensive analysis of cores,seismic and logging data,the depo-sitional filling styles of Palaeogene rifting period in Yuanyanggou area of west Liaohe Depression were divided into 4 second-order sequences and 8 third-order sequences.

运用层序地层学的理论与方法，综合分析岩心、测井以及地震等资料，将辽河坳陷西部凹陷鸳鸯沟地区古近系裂陷期的沉积充填划分为4个超层序和8个三级层序。

Based on the identification of the sequence boundry with the comprehensive analyses of the seismic, well logging and well drilling data, the palaeogene in Jiyang depression can be divided into one lst-order and three 2nd-order sequences, of which six 3rd-order sequences can be subdivided from the third stage of Shahejie formation to Dongying formation.

本文在综合利用地震、声波时差测井、地层倾角测井、电阻率测井、岩心、地球化学等方法进行层序地层界面识别的基础上，将济阳坳陷古近系划分出1个一级层序，3个二级层序，其中沙三段-东营组分出6个三级层序。

The French are not suggesting a new EU treaty, but they have plenty of other wheezes.

法国人并不是在建议签署一个新的欧盟条约，但他们有许多其他的阴谋。

This letter was written by my brother.

这封信是我弟弟写的。