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Cells inosculation was carried out by standard method.The hybridoma supernatants were selected by indirect ELISA.The selected cells were subcloned three times by limiting dilution .Three hybridoma lines against TGEV rMP were obtained , which were designated as 1B3、3C2、4A5. The isotypes of the McAbs were IgG2a、IgG1、IgG1. The three hybridoma lines can secrete antibodies steadily after culturing and saving for a long time.

并以纯化的M蛋白为抗原，免疫BALB/c小鼠，按常规方法进行融合，间接ELISA方法进行筛选，采用有限稀释法，经过三次克隆，最终获得三株抗重组TGEV M蛋白的杂交瘤细胞系(命名为1B3、3C2、4A5)，分泌的单克隆抗体分别为IgG2a、IgG1、IgG1类，三株杂交瘤细胞在体外长期培养和长期冻存时都稳定地分泌单克隆抗体。

New additional features such as weather information, safety spot warners in selected countries, extended country coverage for traffic info service and premium POI content for travel, selected events and cinema

新的附加功能，如气象信息，安全点，华纳在选定的国家，扩大国家覆盖面的交通信息服务和优质蒲的旅游内容，选择活动和电影

Ramin Haerizadeh 1975生于伊朗德黑兰生活与工作在德黑兰 selected SOLO EXHIBITIONS 2008 Men of Allah, GaleriArtist, Istanbul, Turkey 2007 Wonders of Nature, B21 Gallery, Dubai, UAEMelancholy of the Everyday, Studio 1.1, London 2003 Silk Road Gallery, Tehran, Iran selected GROUP EXHIBITIONS 2007 Within and Without, NoMoreGrey Gallery, London 2006 Iran.com, Iranian art today, Museum fur Neue Kunst, Freiburg, Germany ...

Skreber 的画作在介于抽象和写实之间的缓冲带嬉闹玩耍并参加实质性的表演，这场表演似乎更多的是关于放弃用以表现图像的颜色或线条的权宜之计，而非关于讽刺性或者让人发出惊叹的效果。

The location of the anti-kickback pawl relative to the hand guard is selected such when the hand guard is positioned a selected distance from a workpiece, the location of the anti-kickback pawls are automatically set.

这样选择抗反冲爪(36，38)相对手护件(32)的位置，使得当手护件(32)定位在离工件一个选定的距离时，自动给定抗反冲爪(36，38)的位置。

There is a new report stated : the people always selected as parters congiously which selected gene construct and difference person with themself.

巴西一项最新研究表明，人们总是下意识地选择基因构成和自己相异的人做伴侣。

Oil painting: Winter Solstice , selected for the 9th National Art Exhibition; winner of the 1st prize of art works of Guangxi; selected in the exhibition album.

多幅作品发表于《美术》、《中国油画》、《美术界》、《美术大观》、《连环画报》，广西日报、香港文汇报、黑龙江日报等杂志报纸。

With the inlet diameter selected, the shaft speed is selected to limit the inducer tip speed to approximately 550 ft/sec.

与进气道直径选定，轴的速度是选定的限制诱导提示的速度大约550英尺/秒。

When any data has been selected via the interface and the sensor (22) detects a change in the attitude of the mobile telephone terminal or a physical movement thereof, the mobile telephone terminal performs a predetermined operation corresponding to the selected data to be processed.

当已经由该界面选择了任何数据并且传感器(22)检测到移动电话终端的姿态变化或其物理移动时，移动电话终端执行与要处理的所选数据相对应的预定操作。

Mr. Wang Chang has participated in national exhibitions and award-winning, major achievements include: 1999,"the snow-covered gold wind map" was the second capital of the Arts Award for outstanding works Fair,"olgensis First Snow" by the Hong Kong Heritage Museum collection,"Beyond the Great Snow Ji" and three works appeared in the "Contemporary Painting" book,"Snow Hokkoku Ji" by Mr. Ma Ji master comic collection; in 2000,"the North snow bath" is of Jiangsu Wu arts collection,"village day" selected works focus on the Chinese Artists,"Guardian of the North" building seventy-fifth anniversary of the National Excellence Award Exhibition; in 2002,"no place to look for Changbai Waterfall" National Landscape Exhibition selected; in 2004 "the dream of Everest," paintings by the Jilin Province Art Research Association Gold Award; in 2005 "the dream of Everest," the fourth school to participate in snow and ice show; in 2006,"Rural Snow" Exhibition of Fine Arts to participate in the State of Belarus; in 2007,"snow" is the Chinese Cultural Institute collection.

王昌先生多次参加全国性大展并获奖，主要成就有：1999年《雪域金风图》获第二届首都艺博会优秀作品奖，《长白初雪》被香港文化艺术馆收藏，《塞外雪霁》等三幅作品入选《当代绘画艺术》画集，《北国雪霁》被相声大师马季先生收藏；2000年《北国浴雪》被江苏吴门艺术机构收藏，《山村正月》入选中国美术家作品集中，《北国卫士》获全国建军七十五周年艺术大展优秀奖；2002年《长白瀑布无处寻》入选全国山水画大展； 2004年《珠峰之梦》获吉林省书画艺术研究会金奖；2005年《珠峰之梦》参加冰雪画派第四届展；2006年《乡雪》参加白俄罗斯国家美术大展；2007年《融雪》被中华文化学院收藏。

The rabbits were immobilized according to the standard of X_knife before CT scanning. The interrelation of the silica gel tube and bony structures was analyzed, a nerve segment was selected, which could be localized according to the related bony structures as irradiation target. A radiosurgery treatment planning was performed on the selected nerve segment, its changes of pathology and ultrastructure were observed. Results: Base on the dissection, the rabbits' sciatic nerve was composed of the seventh lumbar nerve and first and second sacral nerves.

通过解剖分析兔坐骨神经主干的解剖位置和行径，选择照射靶点，放置硅胶管进行标记，按X_刀标准行CT扫描，从CT图像上分析硅胶管与荐骨和髂骨的位置关系，确定可以用骨性结构定位的神经段作为照射靶点，利用立体定向技术单次大剂量照射坐骨神经，观察其病理及超微结构变化。

Bob Eckert has been chairman and chief executive of Mattel for eight years.

美泰董事长兼首席执行官鲍勃·埃克尔特在任已8年。

The outlook for honors and disgraces carries on and surpasses the traditional outlook for honors and disgraces theoretically, and carries on and develops the theory of construction of socialist mora...

社会主义荣辱观的提出在理论上是对传统荣辱观的一次继承和超越，是对社会主义道德建设理论的继承和发展，在实践上有利于形成良好的社会风尚。