查询词典 sample

Methods: The protein and other impurity in the sample were precipitated by zinc acetate and potassium ferrocyanide after dipping of the sample. N,N-dichlorocyclohexylamine derived from sodium cyclamate by sodium hypochlorite in the filtrate was extracted by hexane. The HPLC separation was carried out with a phenomenex Gemini-C18 column using acetonitrile-water(7+3) or methanol-water (8+2) as mobile phase and detected by UV monitor at 314 nm. External standard method was used for quantification.

样品中甜蜜素浸提后，用乙酸锌和亚铁氰化钾试剂沉淀蛋白质等干扰杂质，过滤，用次氯酸钠将样品溶液中的甜蜜素转化为N，N-二氯环己胺经正己烷萃取后，在phenomenex Gemini-C18柱上，以乙腈－水(7+3)或甲醇－水(8+2)为流动相进行分离，HPLC-UV检测器在314 nm测定，外标法定量。

C blowing air into solution according to a certain flow rate by means of aeration mode, oxidating and breaking sulfocarbon bond, removing organic sulfur from coal, finally filtering treated coal sample and recovering alkaline solution and using it to treat the subsequent coal sample.

本发明在碱性溶液条件催化下，采用低温空气氧化的方法可以高效脱除煤中的有机硫，同时对无机硫的脱除也取得了比较好的处理效果，处理条件温和、处理费用低，同时不会对环境形成二次污染。

Sample 1 had no phenomenon of pulling-out and debonding of fibres under vertical compression load and the interface bonding intensity is strong between the fibres and the matrix. The material shows brittle fracture: if σ(subscript ⊥)=60.7MPa. However, sample 1 has the phenomenon of fibres debonding because of the shearing stress between the fibres and the matrix under parallel compression load. The material shows tough fracture:σ(subscript ∥)=52.6 MPa.

试样l在垂直纤维层方向压缩载荷作用下，没有出现纤维的拔出、脱粘等现象，界面结合较强，材料呈现脆性断裂：σ=60.7MPa；而在平行纤维层的压缩载荷作用下，纤维与基体存在剪切作用，存在纤维的脱粘，试样呈现韧性断裂：σ=52.6MPa。

The pitch refinement algorithm improves the resolution of the pitch from one half sample to one quarter sample around the pitch estimated by HPEM.

基音修正是在HPEM的基础上进一步提高基音估计的精确度，其目的是提高高频谱的拟合程度。

Char sample with different burn off radio were obtain with a tube furnace reactor, burn off radio of the char sample were 0, 30%, 50%, 70% and 100%. Oxygen functionalities of the samples were studied by using XPS method.

分别取污泥质量分数为0、10%、20%、30%和100%配制成5组样品，在管式炉反应器中分别得到各样品在0、30%、50%、70%和100%燃尽率下的焦样。

Results Well-resolved,reproducible 2-DE patterns of SGC7901/VCR and SGC7901 were established.Silver staining was better when protein sample amount was low,overloaded protein will interfere resolution of the maps.Gels stained with colloidal Coomassie brilliant blue had more protein spots numbers and abundance without apparent trails when increased loading protein sample.

结果 获得了背景清晰、重复性好的双向凝胶电泳图谱，两种染色凝胶相比，硝酸银染色在样品少时显示更佳，过量则影响图像质量，而胶体考马斯亮蓝染色在上样量增加时的凝胶蛋白质点数目及丰度均增加，并无明显拖尾。

objective to investigate staining methods for two-dimensional gel(2-de)electrophoresis in multidrug resistance of gastric cancer.methods cultured vincristine-resistant human gastric cancer cell line sgc7901/vcr and its parental cell line sgc7901.variant amount protein of those cells were separated by 2-de.gels were stained with silver nitrate or colloidal coomassie brilliant blue,and scanned by image scanner.results well-resolved,reproducible 2-de patterns of sgc7901/vcr and sgc7901 were established.silver staining was better when protein sample amount was low,overloaded protein will interfere resolution of the maps.gels stained with colloidal coomassie brilliant blue had more protein spots numbers and abundance without apparent trails when increased loading protein sample.conclusion two staining methods were influenced largely by the sum of protein samples,properly selection may be helpful for further study with proteomics in multidrug resistance of gastric cancer.

低甲氧基果胶的胶凝机理及防止预凝胶。。。他扎罗汀凝胶与克林霉素凝胶治疗痤疮。。。注射隆乳后经乳晕切口取出聚丙烯酰胺。。。目的分析利用蛋白质组学方法研究胃癌耐药相关蛋白质中双向电泳凝胶的染色显示。方法培养胃癌细胞sgc7901和长春新碱诱导的耐药胃癌细胞sgc7901/vcr，用双向凝胶电泳技术分离总蛋白，银染及胶体考马斯亮蓝染色，image scanner扫描仪扫描凝胶。结果获得了背景清晰、重复性好的双向凝胶电泳图谱，两种染色凝胶相比，硝酸银染色在样品少时显示更佳，过量则影响图像质量，而胶体考马斯亮蓝染色在上样量增加时的凝胶蛋白质点数目及丰度均增加，并无明显拖尾。结论两种显色方法受样品量影响较大，恰当选用有利于通过蛋白质组学研究胃癌耐药机制工作的进一步开展。

The sample data of impinging pressure of irrigating water column impinging to seedbed furrow can been direct collected by computer,and the water power characteristic of impinging water column can been studied by analyze the sample data.

要解决这一问题、彰显穴灌坐水播种的优势就必须正确认识灌溉冲击水柱在沟内的水力特性，特别是灌溉水注对种沟沟底的冲击压力特性。

Be familiar with the sample trial procedure, execute tooling setting for sample trial, and solve on-site problems during the manufacturing in time.

熟悉样品试制流程，完成试生产调机工作，并及时解决现场出现的生产加工问题

Refrigerating engineer: Lead the refrigerating group to develop new refrigerating system, improve the performance of current refrigerating system, Responsible for the testing of engineering sample and trial run production sample, provide test result, and confirm final refrigerating configuration.

Job Summary工作摘要：领导制冷小组负责开发新的制冷系统，改进当前的产品性能，负责对工程样品和试产样品进行制冷性能实验，总结测试结果，并由此确定制冷系统的配置。

Don not attempt to do something which you can not to do.

不要企图做那些办不到的事情。

The expression of CTGF and TNF-αweredetected by immunochemistry and the number of Clara Cells was calculated.

光镜下观察肺组织的病理变化，采用免疫组化染色观察肺组织中结缔组织生长因子和肿瘤坏死因子-α的表达和Clara细胞的数量。