查询词典 regulatable

Transgene expression could be efficiently controlled by using the inducible lentivectors incorporating regulatable system,which expands the potential of lentivectors for a wide array of clinical gene transfer application, therefore it becomes a potential viral vector in gene therapy.

然而，发展应用于临床基因治疗的慢病毒载体，要求能够严格控制目的基因的表达，并确保治疗有效性和减少毒副作用。

Secondly,two regulatable current sources for exicitation of magenetic powder dynamic brake are studied respectively with buck and interleaving two-transistor forward topology. In the power supply with interleaving two-transistor forward topology design,simulations are implemented with Saber simulator,thus the best parameters are achieved which ensured the stability and dynamic characteristic.

二、分别用 BUCK 式和交错并联的双管正激式电路拓扑，研制了磁粉制动器的直流励磁电流源，其中对于交错并联双管正激式的电路进行了基于 Saber 的仿真，确保了电路参数的选取能够保证电路稳定性与动态性能要求。

The expression of AT2R gene can be regulated efficiently in vivo by local delivery of MSC with dual-stable expression of AT2R gene, thus reduces the formation of intimal hyperplasia.④AT2R gene can influence the expression of ECM and significantly promote the cellular apoptosis in the injury rat carotid artery.⑤These data demonstrate the clinical potential of AT2R regulatable expression to prevent restenosis after PCI.

研究结论：(1)与VSMC直接接触可以诱导MSC向血管成分细胞分化；(2)构建的Dox-on可调控的MSC细胞株诱导活性可靠，使外源AT2R基因的表达处于有效的主动控制下；(3)局部导入MSC不影响球囊损伤后大鼠颈动脉新生内膜增生；(4)由MSC介导的AT2R可调控表达受到Dox的良好调控，可有效抑制球囊损伤后大鼠颈动脉新生内膜增生；(5)AT2R影响血管损伤后修复时ECM成分的表达，并促进细胞凋亡的发生，可能是AT2R介导抑制新生内膜形成的机制。

TUNEL method was used to detected the apoptosis in the artery. Results:①The MSC cocultured with VSMC expressed smooth muscle a-actin, calponin and CD31, no cells positive for calponin and CD31 were detected in the control group; and a lot of filaments were observed in the co-cultured MSC by electron microscopy.②We gain dual-stable expression of AT2R gene medicated by doxycycline regulatable system of mesenchymal stem cells.

单独培养及VSMC条件培养液培养的MSC仅SM-α-actin表达阳性；(2)Dox-on系统由四个质粒组成：调节质粒pUHD17-1 hyg，荧光报告质粒pUHC 13-3，筛选质粒pSV2neo以及含目的基因AT2R的应答质粒pUHD10-3/AT2R，以上质粒经鉴定均与其背景资料相符合，可用于实现目的基因片段在靶细胞中可调控表达研究；(3)本实验通过连续两个回合的转染及抗生素压力筛选，经Dox诱导表达后获取出低背景、高诱导表达AT2R基因的克隆。

I hope that this success is just the first in a long series.

我希望这只是成功的开始。

Three dimensional stresses on the interface of tire and soil, tire deflection and soil sinkage are measured.

测试轮胎——沙土界面上的三向应力、轮胎变形和沙土沉陷；建立考虑剪切应力对沙土承载特性产生影响的车轮牵引性能预测模型；分析车轮作用下土壤的纵向稳定性。