查询词典 regulatable

In our laboratory a chemically regulatable male sterile expression system had been constructed based on the antisense actin strategy and putatively transgenic tobacco plants with Kan-resistance had been obtained.

本实验室在自行研究成功的反义肌动蛋白雄性不育策略的基础上，构建成由铜调控的在绒毡层细胞特异表达anti-actin基因的植物表达系统，转化烟草获得由kan抗性筛选的烟草植株。

This thesis tries to set up a effective regulatable mechanism to deal with terrorism crime by using the method of culture study.

本文试图从文化研究的视角出发，构建一个可行之有效的对恐怖主义犯罪的调控机制。

To construct a expression vector induction by RU486 and confirm the regulatable effect in vitro.

构建可经RU486诱导表达载体，并证实其对基因表达的调控作用。

We demonstrated the regulatable expression of this vector after transfection in HEK293 cells by fluorescence microscopy and flow cytometry.

在转染HEK293细胞后，运用荧光显微镜和流式细胞技术证实了该载体的调控能力。

Fluorescence microscopy and flow cytometry were used to observe the activation of this regulatable vector after transfection in MFC cells.

在转染MFC细胞后，运用荧光显微镜和流式细胞技术检测该载体的调控表达。

Our experiment has laid a foundation for the further study of in vivo regulation of TPO gene expression, it is likely to provide a safe and regulatable way for TPO gene therapy.

所建立的逆转录病毒整合的NIH3T3细胞株，可利用四环素及其衍生物对其进行定时，定量调节多种外源基因的表达，从而增加基因治疗的精确性和有效性。

Our experiment has laid a foundation for the further study of in vivo regulation of TPO gene expression, it is likely to provide a safe and regulatable way for TPO gene therapy.

本实验为进一步进行TPO基因表达调控的体内研究奠定基础，有望为TPO基因治疗提供一条可控的安全途径。

Usually, the interconnections can be devided into two parts: fixed part and regulatable part. The regulatable part can be assigned directly in an open-loop way while the closed-loop feedback control is implemented in a coordinate way.

因此，可将系统关联关系分为两个部分：固定部分和直接可调部分，可以针对可调部分进行开环的直接调节，并与闭环反馈控制协调使用。

Objective: The purposes of the present study are as follows: 1 To study the effect of direct contact with VSMC on transdifferentiation of MSC; 2 To construct MSC of Dual-stable expression of AT2R gene medicated by tetracycline regulatable system.; 3 To investigate the effects of AT2R gene medicated by tetracycline regulatable system on neointimal hyperplasia in rat carotid arteries after balloon angioplasty.

本实验体外观察MSC与VSMC在直接接触培养下的分化情况，并以MSC为载体，通过体外细胞基因转染及筛选，获得双重稳定受到强力霉素调控表达AT2R基因的MSC，将此受到调控表达的AT2R基因导入大鼠颈动脉球囊损伤动物模型中，观察MSC细胞移植实现AT2R基因在体可调控表达在新生内膜中形成的作用及潜在意义。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

This is not an ordinary box, which is used as a background picture of the dialog box, very pretty.

详细说明：这可不是一个一般的对话框，它是用图片作为背景的对话框，非常好看。

Conceal me what I am,and be my aid for such disguise as haply shall become the form of my intent.

遮掩我的身份，帮助我，我的面具将成为我的目的。