查询词典 real time

Objective To develop a real-time fluorescent quantitative PCR for rapid detection of aspergillus in blood.

目的 建立并评价从血标本中检测曲霉菌基因的real-time PCR方法。

In this study, real-time PCR technology was used to dectect the expression of PRL mRNA and its receptor in the reproductive tissues,which is hypothalamus-pituitary-ovary-oviduct four important tissues. The aim was to compare the difference of PRL mRNA expression in the different state of ovulatory, broody and after broody, and to analyse the relation between expression and reproduction.

本试验利用real-time PCR技术检测雪山草鸡下丘脑、垂体、卵巢、输卵管四个繁殖轴组织中PRL基因及其受体的表达量，以确定处在生产、抱窝、醒抱三个不同状态母鸡PRL基因表达的差异，并分析基因表达量的差异与生产性能的相关性。

To study the role of FoxO1 on myoblast proliferation and differentiation and the transformation of muscle fiber type in porcine,here we use yorkshire as experimental materials and detect the expression of genes related myogenesis and muscle fiber type in pporcine myoblast that was knockout or activated FoxO1,adopting the technology of RNAi,real time-PCR and Western blotting et al..

为探讨FoxO1对猪成肌细胞增殖分化及肌纤维类型转化的作用及机理，本试验以大白猪为研究材料，利用RNAi、real-time PCR、免疫细胞化学和Western blotting等技术，通过在原代培养的猪成肌细胞中敲除和激活内源活性FoxO1，检测肌肉形成与肌纤维类型相关基因的表达。

Take SYBR Green I as the intercalating fluorescence dye in Real-time PCR,the overexpression of WIPK and SIPK,which were the key genes of MAPK cascades,and the resistant gene PR1 in tobacco treated with copper and magnesium solution were studied.

采用SYBR GreenⅠ作为荧光染料的Real-time PCR方法，明确了铜、镁素能调控烟株体内蛋白激酶级联信号转导途径的关键基因WIPK、SIPK及抗病信号通路末端PR1基因的过量表达，进一步揭示了矿质营养调控烟草对病毒抗病性的分子机制。

Bifidobacteria in feces samples can be directly quantitated or semiquantitated by real-time PCR method or common PCR method.

利用PCR、real-time PCR直接半定量和定量粪便中的双歧杆菌可行。

Therefore it is interesting to clone genes related to lemma and palea. In this thesis, six genes from the lemma/palea related gene pool which is established by Liu (2003) were further studied. Those genes are salT gene (salt-induced protein), GA-SPY gene (gibberellin action negative regulator SPY-related protein), U2AF gene (U2 snRNP auxiliary factor, small subunit-related protein), kinesin-like gene, DnaJ-like gene, and EF hand gene. At first, the gene expression in leaves and 1~4 cm inflorescences of normal lemma/palea, smaller lemma/palea, and stunted lemma/palea was compared by Real Time RT-PCR.

从刘(2003)所建立水稻颖花发育相关候选基因库中，挑选6个基因：salT基因(salt-induced protein)、GA-SPY基因(gibberellin action negative regulator SPY-related protein)、U2AF基因(U2 snRNP auxiliary factor，small subunit-related protein)、kinesin-like基因、DnaJ-like基因、及EF hand基因，以水稻正常型颖花、小颖花突变体及内外颖退化突变体之叶片及1~4公分幼花序为材料，利用Real-Time RT-PCR分析，发现salT基因在突变体幼花序中大量表现。

Giemsa staining was used to observe the growth of C.andersoni,and transmission electron microscope was used to observe the developmental stages of C.andersoni.MTT assay was used to evaluate the toxicity of nitazoxanid and ginkgoic acids to HCT-8 cells.C.andersoni parasites growing in HCT-8 cells were treated with different concentrations of NTZ and GAs,and the copies of COWP gene were determined by Real-time PCR.

安氏隐孢子虫卵囊感染人结肠癌(HCT-8)细胞，Real-time PCR技术测定RPMI-1640培养液中分别加入不同浓度胎牛血清(fetal calf serum，FCS)、葡萄糖(glucose，GLU)、维生素C(ascorbic acid，ASC)、叶酸(folic acid，FOLIC)、泛酸钙(calcium pantothenate，CAPAN)和胰岛素(insulin，INSULIN)后虫体的增殖效果。

In mouse peritoneal macrophages. Methods Mouse peritoneal macrophages were cultured in the presence of heat-killed yeast-phase PM for 24 h, and the average fluorescence intensity of TLR-2, TLR-4, and Dectin-1 in the macrophages was detected using flow cytometry. Fluorescent staining of the macrophages was performed to observe the fluorescence of TLR-2, TLR-4, and Dectin-1 with confocal microscopy. TNF-α mRNA in the cell culture supernatant was measured with real-time PCR, and TNF-α protein detected using enzyme-linked immunosorbent assay.

马尔尼菲青霉酵母相菌液与小鼠腹腔巨噬细胞共培养24h，采用流式细胞技术检测巨噬细胞TLR-2、TLR-4及Dectin-1的平均荧光强度；共聚焦显微镜观察荧光染色的受体；ELISA法测定培养液上清中TNF-α的浓度；Real time PCR检测不同时间段TNF-α的mRNA表达。

The established event-specific real-time PCR detection systems in this study are suitable for the identification and quantification of these GM maizes.

再辅以即时聚合酶链锁反应( real-time PCR )检测基因改造玉米含量，将有助於建立基因改造玉米产品标示管理。

However, RFID stands for Radio Frequency Identification, and is also called as Electronic Label. It goes through radio signals to identify specific targets, and reading and writing information, as well as don't need identity system and specific targets to create such as electronic, optics or machinery. Properties of wireless communication, space-penetrating, information tracking and real-time location system/ RTLS will apply for increasing the system capability of safety and protection to Robot.

而「RFID， Radio Frequency Identification 」称之为「无线射频识别」，又称「电子标签」，是一种通信技术，可透过无线电讯号识别特定目标并读写相关资料，而无需识别系统与特定目标即可建立含电子、光学或机械接触，其特性：无线通讯、空间穿透性、资讯历程可追踪与「即时定位系统 RTLS， Real Time Location System」等，必然将成为机器人、机械手臂安全设计与防护的新应用。

Objective To investigate the effects of interleukin-1 β converting enzyme gene on the biologic characteristics of ovarian cancer cells.

目的 探讨白细胞介素-1 β转换酶的表达，对卵巢癌细胞生物学特性的影响。

Campylobacter: This illness is the most commonly identified cause of diarrheal illness in the world.

弯曲：这种病是最常见的原因查明腹泻病，在世界上。