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The content of Happy Cuber is based on the geometric learning theory, and 3D real-time rendering is used to provide the freely using of viewing angle control. Besides, the game factors such as competition, conflict and reward, are embeded in the courseware in order to arouse students' interest in studying.

教材内容的设计是以空间几何学习理论为基础，搭配三维即时描绘(3D real-time rendering)来提供视角自由操作，并融入数位游戏式学习的内涵来提高学童的学习兴趣。

Quantitative method for mRNA level of IBP: The standard preparation of objective gene IBP and house-keeping geneβ-actin were used to get standard curve of real time PCR.

mRNA水平定量方法：利用IBP和管家基因β-actin的质粒标准品绘制real time PCR标准曲线，建立检测IBP的real time PCR标准曲线定量法。

To establish a real-time PCR method for the rapid detection of Listeria monocytogenes in simulated clinical specimens.

本研究利用荧光定量PCR(real-time PCR)技术，建立针对临床标本中单增李斯特菌的快速检测方法。

Lysis solutions from imported and domestic genomic DNA purification kits,and 2% Trition X-100 and 5%guanidinium isothiocyanate were added into the oocysts,followed by different combining forms of freeze-thawing,proteinase K and sonication.Real-time PCR was used to determine the copies of Cryptosporidium oocyst wall protein gene.HCT-8 cells were infected by C.andersoni oocysts.Real-time PCR was used to determine the enhancement effect of fetal calf serum,glucose,ascorbic acid,folic acid,calcium pantothenate and insulin while added into RPMI-1640 medium.

在隐孢子虫卵囊悬液中分别加入进口试剂盒和国产试剂盒的裂解液以及2%Triton X-100和5%异硫氰酸胍，辅以不同组合方式使用的冻融法、蛋白酶K法和声裂法裂解卵囊，通过Real-time PCR法测定裂解产物中隐孢子虫卵囊囊壁蛋白(Cryptosporidium oocyst wall protein，COWP)基因拷贝数。

METHODS: RAW264.7 macrophages growth inhibition was measured by MTT assay. The apoptosis effect of RAW264.7 murine macrophages induced by ox-LDL was analyzed by flow cytometric analysis and DNA agarose electrophoresis. After expression of Daxx was silenced by special siRNA, the macrophages apoptosis was observed by AO/EB fluorescence staining. Oil Red O Dyeing experiment was used to show the cellular lipid droplets in lipid-loaded cells. High performance liquid chromatography analysis was performed to determine the content of cellular cholesterol. Real time RT-PCR was used to detect the mRNA expressions of Daxx and SCAP.

用不同浓度的氧化型低密度脂蛋白处理RAW264.7细胞48h，MTT法检测ox-LDL对RAW264.7细胞生长的影响；流式细胞术和DNA断裂片段分析法研究ox-LDL诱导的细胞凋亡；用特异性siRNA沉默Daxx在RAW264.7细胞中的表达，通过AO／EB染色观察基因沉默后的细胞凋亡形态学改变；高效液相色谱检测细胞内胆固醇含量；油红O染色观察细胞内脂滴的形成情况；Real time RT-PCR检测细胞内Daxx mRNA、SCAP mRNA的表达情况；用Western Blot印迹法检测caveolin-1蛋白的表达。

Real Time Controls Laboratory - RTiC-Lab is a semi-detached, open source software designed to run on both Linux and RTLinux.

Real Time Controls Laboratory是一个半分离的，开源软件，设计为可以在Linux 和RTLinux上运行。

RT-PCR and Real-time PCR analysis revealed that mRNA levels of the FK506-binding protein 12 (FKBP12) and two unknown genes were higher in the diapausing pupae, whereas the ecdysteroid-regulated 16kDa protein (esr16), NADH dehydrogenase 1 alpha subcomplex subunit 6, and two unknown genes were higher in the pupae of diapause termination.

通过RT-PCR和Real-time PCR的方法，鉴定了滞育解除过程中有3个基因表达量在下调，有4个基因表达量在上调。这7个差异表达基因中有3个和已知的基因有较高的同源性：FKBP12，esr16和NADH dehydrogenase 1 alpha subcomplex subunit 6。

Methods Five groups (n=24) of SD rats were randomly assigned to received intrapleural injection of dexamethasone, dipopolysaccharide, erythromycin, hypertonic saline and normal saline, respectively. The AQP1 protein in pleural was detected with immunohistochemistry. The mRNA expression of AQP1 under stimulations at different time points was measured by real time RT-PCR.

SD大鼠按实验设计分为5组(n=24)：地塞米松组、内毒素组、红霉素组、高渗盐水组及对照组，通过免疫组织化学染色测定AQP1在胸膜上的表达和定位；采用Real-time RT-PCR方法对不同刺激因子作用下不同时间胸膜组织AQP1的基因表达进行定量分析。

Objetive:To investigate the histopathological features and detect the mutation of JAK2V617F in PV、ET and IMF;to analyze the effects of the stages of myelofibrosis and the mutation on the number of leucocyte in PV and ET patients;Methods: the morphological features of 69 patients who dignosed in the first time were studied by HGF and Gomori stains ; the mutation of JAK2V617F was detected in 38 blood or marrow samples of PV、ET and IMF patients by real-time PCR;the effects of the stages of myelofibrosis and the mutation on leucocytes were analyzed;Results: Different stages of reticulin were observed in 15 PV and 15 ET patiens'marrow sections,and the cytosis、the pleomorphism and the bone trabecula side location of megakaryocytes were observed in all PV、ET and IMF patients;the means of leucocyte in patients with and without JAK2V617F were 20.4×109/Land 11.4×109/L respectively;the P value was 0.367 as comparing the leucocytes among different stages of reticulin increasing.

目的：探讨PV、ET、IMF患者的骨髓病理特点并且检测JAK2V617F突变的发生率，分析骨髓中纤维增生程度以及JAK2V617F对PV、ET患者白细胞数的影响；方法：69例初诊患者的骨髓切片经HGF染色以及Gomori染色后进行形态学研究；并利用real-time PCR法测38例患者的骨髓或者外周血的JAK2V617F突变；分析骨髓切片中纤维组织增生程度以及JAK2突变对患者白细胞数的影响；结果：15例PV和15例ET患者的骨髓切片中可以观察到不同程度的纤维组织增生，所有PV、ET、IMF患者骨髓病理中可见到巨核细胞增多、巨核细胞多形性改变以及巨核细胞定位于骨小梁旁；12例PV、4例ET、4例IMF中检测到JAK2V617F；有突变和无突变患者发病时白细胞数均值分别为20.4×109/L和11.4×109/L，比较不同纤维增生程度的PV、ET患者的白细胞数均值，P值为0.367。

The real-time PCR used SYBR GreenⅠsystem as control group to compare semi-quantitative PCR system. When detecting a lot of samples, semi-quantitative PCR system could separate them from more than 7% or less than 3% to decrease the numbers of samples for quantitating by real-time PCR.

实验同时使用real-time PCR之 SYBR Green Ι法作为对照检测系统，以此系统与半定量PCR检测系统比较，於大量检测样品时，可将样品初步区分基因转殖含量低於3%及高於7%，以减少必须使用real-time PCR定量之样品数，进而可缩短检验的时间及降低检验所需费用。

Graf, without question, is the greatest women's player in history.

格拉芙，现在，毫无疑问是女子网坛历史上最伟大的选手。

If not, you will have to look around at all your important data.

如果没有，您将需要检查所有的重要数据。