查询词典 ras

Objective To decide the base sequences of target position for gene therapy, the K-ras point mutation and its style at codon 12 in human pancreatic cancer cell line Patu 8988 was detected.

目的 检测人胰腺癌细胞株Patu 8988 K-ras基因点突变及其突变方式，明确基因治疗靶点的碱基序列。

The experiment shows that human pancreatic cancer cell line Patu 8988 has GGT→GTT mutation at codon 12. The method of PCR-SSP is rapid, convenient, economical and high specific for detecting K-ras gene point mutation. The goal in the long term is to develop a simple and useful clinical procedure for early diagnosis and differential diagnosis of pancreatic cancer.

明确了人胰腺癌细胞株Patu 8988 K-ras基因第12位密码子点突变方式，为胰腺癌的下一步基因治疗研究打下了坚实的基础；PCR-SSP方法检测K-ras基因点突变简便、快速、经济且特异性高，有望在临床上成为早期诊断和鉴别诊断胰腺癌的实用检测方法。

Methods K-ras gene point mutation and its style at codon 12 of Patu 8988 cell were detected by using PCR-SSP and sequence analysis.

方法顺序特异引物聚合酶链反应法和基因测序检测Patu8988细胞K-ras点突变形式，根据点突变形式设计并合成硫代反义寡核苷酸（K-ras mutation ASODN）作用Patu 8988，通过噻唑蓝比色法检测细胞生长情况；流式细胞术检测K-ras蛋白表达和细胞凋亡；逆转录-聚合酶链反应检测K-ras mRNA表达水平；以Patu8988细胞建立裸鼠胰腺癌模型观察K-ras mutation ASODN在体内的抗肿瘤效果。

Methods Three kinds of special sequence primers for polymerase chain reaction with regard to the mutation styles (CGT、GTT、GAT) at codon 12 of K-ras were used to study the human pancreatic cancer cell line Patu 8988.The amplification products were studied with 12% polyacrylamine gel eletrophoresis to detect the style of point mutation.

针对K-ras基因第12位密码子点突变方式(CGT、GTT、GAT)设计顺序特异性引物，对人胰腺癌细胞株Patu 8988进行聚合酶链反应，扩增产物经12%非变性聚丙烯酰胺凝胶电泳后判断该细胞株有无K-ras基因点突变及其突变方式。

Erve growth factor combined with its receptor, activated Ras - MAP kinase , mitogen - activated protein kinase kinase is the important regulating factor that make IkB kinase, IKK, phosphated, IkB kinase make the IκBα:(the subunit of NF -κB ) phosphated, the phosphated IkBα degraded, and p65 - p50 heterodimer can be formed, then the heterodimer translocated to nucleus and combined with the promoter domain or other consensus sequence.

GF与其受体相结合，最后可以激活Ras-有丝分裂激活的蛋白激酶途径，有丝分裂激活的蛋白酶激酶(MEKK1)是IγB激酶发生磷酸化的重要调节因子，IKK使NF-κB的亚单位IKBα发生磷酸化，磷酸化的IKBα发生降解，而最后留下p65-p50二聚体，该二聚体然后转位到细胞核内与κB基因的增强子区域或与他的顺式作用序列结合。

Methods Expression of the survivin mRNA was evaluated by reverse transcriptase polymerase chain reaction in 76 NSCLC tumor samples,20 benign phymatoid lesion and 21 adjacent normal lung tissue samples Immunohistochemical assay was to detect the expression of P53,c myc,k ras proteins.

研究细胞凋亡的调节机制对于弄清肿瘤包括肺癌的发病机制及新的防治方法具有重要意义。survivin是最近发现的一种凋亡抑制蛋白，在已检测的几种肿瘤组织中表达上调[1 3] ，在肺癌中的表达目前报道甚少，其与

C. When the simulation results exported from HEC-RAS is imported back into HEC-GeoRAS model of ArcGIS, the results will present the flood areas of each returned period. Besides, in the diagram, the rationality of the physiographical data and the simulation results from the cut cross sections can be discussed to cut more cross section or not.

由HEC-RAS之水理演算所获得成果於转汇回ArcGIS之HEC-GeoRAS后，可展现各重现期距之洪水到达区域，且由其图示更可讨论切制断面所获得之地文资料及其水理演算成果之合理性，并判释是否须加密切制其断面。

This paper introduces a new method by taking full advantage of Internet and RAS technology, which can implement remote medical treatment prefectly. This method has been proved to be practical and will facilitate chinese hospital digital and information construction.

介绍了一种通过Internet和RAS实现远程医疗的解决方案，该方法切合我国目前的国情，可以较好地实现远程诊断，又有助于实现医院医疗系统的数字化、信息化。

It is practical that using the spermatogonial cells as the vector to produce the transgenic mice of HBx and ras gene, and could play a role in establishing transgenic animal model of tree shrews.

以精原细胞作为载体，建立带HBx及ras基因的小鼠模型是可行的，为建立树的转基因动物模型的研究打下基础。

Results Left and right RAS were 18 and 30 cases. The stenotic cases were slight 26, moderate 15 and severe 7 respectively.

结果 发现左、右RAS各为18、30例，轻、中、重度狭窄各26、15、7例；5例行RAS扩张成形介入治疗。

We stopped by the old fashion ice cream parlor Manhattan Beach Creamery for some refreshment. The "No Shoes, No Shirt, No Problem" sign on the wall epitomizes the beach lifestyle.

我们去商店区的一家老式冰淇淋店坐坐，墙上挂著「没鞋，没上衣，没问题」的牌子完全捕捉了海滩生活的随性及风格。

"If therefore", argues Bellarmine we can offer our prayers and our satisfactions in behalf of those detained in purgatory, because we are members of the great body of Christ, why may not the Vicar of Christ apply to the same souls the superabundant satisfaction of Christ and his saints--of which he is the dispenser?

" "因此，如果"，认为贝拉明（德indulgentiis ，十四）"我们可以提供我们的祈祷和我们满意的代表那些被拘留在炼狱，因为我们是成员的伟大基督的奥体，为什麼可能不是牧师基督适用於同样的灵魂满意的过剩是耶稣和他的圣人-他是在配药？