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BmK IT2 associateed with and dissociated from its binding sites on insect synaptosomes quickly in two phase kinetic manner, and its specific binding was independence of membrane potential, but could be significantly inhibited by native BmK IT2, BmK AS and BmK AS-1 in a dose-dependent manner, and only partially by BmK Ⅰ or veratridine at high concentration.

BmK IT2能以两相动力学方式快速与昆虫突触体结合和解离，且其结合不依赖于膜电位，可分别被天然毒素BmK IT2，BmK AS和BmK AS-1剂量依赖地抑制，另也可被高浓度BmK Ⅰ和藜芦碱部分抑制。

A civil obligation is one which has a binding operation in law, vinculum juris, and which gives to the obligee the right of enforcing it in a court of justice; in other words, it is an engagement binding on the obligor.

民事责任是一个具有法律约束力，vinculum操作，并给出两个向债权人正确执行它在法庭的；换句话说，它是一个订婚的消息对债务人。

Study on the binding site and binding amount of a number of antirumor metal complexes, cisplatin, Cp2TiCl2 and (CH3)2SnCl2, to target molecule mononucleotides dGMP in aqueous solution, by virtue of a 1H NMR recognition probe,trans-[en2Os(η2-H2)(CF3SO3)](CF3SO3) in a competitive mode.

具有抗肿瘤活性的试剂作用的靶分子可能是核酸和蛋白，用此锇胺分子氢络合物作为探针，在竞争模式下，研究了具有抗癌活性的Me_2SnCl_2与丙氨酸的作用，并得出了它们的最小结合量。

After the identification of SARs in Attacus ricini rRNA gene, its rRNA gene and NTS were also found to have the ability binding with the nuclear scaffold of Saccharomyces cerevisiae. The 1kb NTS fragment which can bind clearly with nuclear scaffold of Attacus ricini was the chief fragment binding with yeast nuclear scaffold. This indicated the homology between this two kinds of SARs and provided an evidence for the evolutionary conservation of SARs.

在鉴定蓖麻蚕rRNA基因中具有骨架结合区域之后，利用体外结合的方法发现，蓖麻蚕rRNA基因及其非转录间隔区片段也具有与酵母核骨架结合的能力，尤其是能与蓖麻蚕核骨架结合的非转录间隔区1kb片段，也能明显地结合于酵母核骨架，这一结果表明二者的SAR顺序具有一定的同源性，为骨架结合区域具有进化保守性提供了又一证据。

Chapter 5: The fluorescence quenching reactions of barbaloin with serum albumin in solution were studied. The quenching mechanism of serum albumin by barbaloin was interpreted using the Stern-Volmer mechanism. The binding constant K values and the number of binding sites were calculated, respectively.

第五章：首次研究了芦荟甙与血清蛋白在溶液中的相互作用，利用Stern-Volmer方程解释了芦荟甙对血清蛋白的荧光淬灭，并测定了不同温度下的结合常数及结合位点数。

Absorption spectral studies show that binding to the serum albumins leads to a bathochromic shift of the monomer band together with a notable intensity change. Furthermore, the number of binding sites was identified by the absorption spectra.

吸收光谱数据表明，与血清蛋白结合后，噻菁染料单体的吸收峰发生红移，同时强度也有很大变化；还通过吸收光谱计算确定了噻菁染料与血清蛋白的结合位点数。

Seven colorimetric methods Biuret, Lowry, a modified Lowry technique using bicinchoninic acid, Coomassie brilliant blue G (CBBG dye-binding in phosphoric acid, perchloric acid or hydrochloric acid, and bromophenol blue dye-binding were evaluated for determination of protein concentration in human whole saliva.

本实验以七种定量蛋白质之方法（Biuret法，Lowry法，BCA法，CBBG染料结合法、BPB染料结合法），利用三种参考蛋白质评估测定人类唾液蛋白质浓度之差异性。

Using HPLC separation and receptor binding assay, the present study demonstrated that the principle in the water extract of Huangqin acted on the BDZ binding site was not baicalin, wogonin or chrysin. In vivo study, WEH had no significant effect on pentylenetetrazol-induced clonic seizures in mice, although it could inhibitmaximal electroshock-induced tonic seizures with an ED50 of 3.6g/Kg.

本研究中，藉由高效率液相层析术与接受器结合实验评估，证实黄苓水抽提物中作用在BDZ结合处之主要成分，并不是黄苓甘、泛黄苓素或chtysin。

All experimental results show that the mode of binding of Sm3 to DNA is mainly electrostatical binding.

实验综合表明，Sm3与DNA之间的结合主要为外部静电作用方式。

Objective To express and locate a fusion gene consisting of c-Myb DNA-binding domain and Myeloid elflike factor AML1-binding region by using baculovirus vector.

目的 将C-Myb的DNA结合域与MEF的AML1结合区组合成的MM融合基因在杆状病毒中进行表达和细胞定位。

Don not attempt to do something which you can not to do.

不要企图做那些办不到的事情。

The expression of CTGF and TNF-αweredetected by immunochemistry and the number of Clara Cells was calculated.

光镜下观察肺组织的病理变化，采用免疫组化染色观察肺组织中结缔组织生长因子和肿瘤坏死因子-α的表达和Clara细胞的数量。