查询词典 protoplast

The results showed that the Saccharopolyspora spinosa SP06081 protoplast yield was the highest under these conditions: the collected mycelium from SP06081 grown in Tryptic Soy Broth medium with 0.2% glycin for 48 h was treated by 0.1 mg/mL lysozyme at 28oC for 20 min, then plated on the R2YE medium with sucrose as osmotic stabilizer, the number of regeneration protoplast was up to 108/mL. The protoplast-regenerated strains exhibited changes in morphology and antibiotic production, 29.3% protoplast-regenerated strains was characterized by loose mycelium and abundant broken branches as did their parent. Among them, 58.2% strains presented the trend to positive variation in spinosad yield, with the highest spinosad yield of up to 582.0 mg/L, 85.6% higher than that of their parent.

结果表明：菌体在添加0.2%的甘氨酸的TSB培养基中培养48 h收集， 0.1 mg/mL 溶菌酶， 28oC作用20 min制备原生质体，将原生质体涂布于以蔗糖为渗透压稳定剂的R2YE培养基中，原生质体再生数目最多，达108个/mL以上；原生质体再生菌株在形态和抗生素产量上产生分化， 29.3%的再生菌株形态上保持与亲本菌株一致，具有菌丝松散，断裂分枝多的特点，其中53.2%的再生菌株多杀菌素产量变异向正方向移动，最高产量达到582.0 mg/L，比亲本菌株提高85.6%。

The best combination of isolating protoplast of Mozzie buster was the enzyme mixture containing 2.0% Cellulase R-10, 0.4% Pectinase, 0.5M mannitol as osmoticpressure, 27℃under dark 80r/m rotating enzymolysis 8h, and the highest yieldachieved to 1.67×10~6per gram, the protoplast vitality was 87.4%; The bestcombination of isolating protoplast of Ocimum basilicum was the enzyme mixturecontaining 1.6% Cellulase R-10, 0.4.% Pectinase, 0.5M mannitol as osmotic pressure,27℃under dark 80r/m rotating enzymolysis 8h, and the highest yield achieved to1.67×10~6 per gram, the protoplast vitality was 87.4%.

本研究对酶解最佳条件进行研究，得出分离驱蚊草原生质体酶解条件以CPW-13进行质壁分离1h，2.0％纤维素酶和0.4％果胶酶，0.5M甘露醇为渗透压，27℃黑暗下80r／m酶解处理8h为好，产量可达1.67×10~6个／g，原生质体活力为87.4％；分离罗勒原生质体酶解条件以CPW-13进行质壁分离1h，1.6％纤维素酶和0.4％果胶酶，0.5M甘露醇为渗透压为好，27℃黑暗下80r／m酶解处理8h，产量可达2.14×10~6个／g，原生质体活力为67.8％。

To find out the suitable medium, the optimization groups of hormone of NAA content and ZT content and the appropriate cultural density, the protoplast of two species of Jujuba trees have been cultivated by taking as subject the protoplast of non-kernel small date and egg-like dates embryonic suspension cells obtained by zymolysis, and by employing different mediums with different combination content of NAA, ZT hormones and culture density of protoplast.

以无核小枣和鸡蛋枣两个枣树品种的胚性悬浮细胞经酶解获得的原生质体为材料，采用不同种类的培养基、不同含量的NAA和ZT激素组合及不同原生质体培养密度对两个枣树品种的原生质体进行培养，以获得适宜的培养基种类、较佳NAA和ZT的激素组合及适宜的培养密度。

Since Arabidopsis thaliana guard cell protoplast is quite small (6~9μm), it had brought lots of difficulty to protoplast isolating and patch clamping.

由于拟南芥保卫细胞原生质体个体较小(6～9μm)，给分离提取保卫细胞原生质体带来了一定的困难。

The latest developments on protoplast culture and protoplast fusion are focused in this review.

本文综述了近年来世界各国果树原生质体培养和细胞融合的研究进展。

The protoplast fusion between hyperparasitic Streptomyces strain F46 and Actinomyces strain SC11 was studied in order to improve the effect of wild actinomyces strains to control plant diseases, solve the problems in application of the biocontrol strains, explore the method of protoplast fusion and get the recombinants with some excellent characteristics of both parents.

为了提高野生生防放线菌株的防病效果，克服以往生防菌应用方面存在的诸多弊端，探索简单易行的人工改良生防放线菌的途径，我们选用重寄生链霉菌F46和放线菌SC11作为亲本，进行了双亲原生质体融合研究，以期得到整合双亲多种优良性状的工程菌株。

On the basis of protoplast culture, we performed protoplast fusion between Astragalus melilotoides Pall, and Zygophyllum xanthoxylum Maxim using a modified PEG high-pH, high-Ca2 method. The protoplasts of A. melilotoides were pretreated with Rhodamine-6G and those of Z.

在成功培养原生质体的基础上，用改进的PEG-高pH高钙法诱导草木樨状黄芪和木本霸王原生质体融合，得到了科间体细胞杂种融合细胞。

In the mating test, mycelial protoplast pairs between single spore isolates of different strains formed colonies with clamp connection, while mycelial protoplast pairs between the same single spore isolates formed colonies without clamp connection, suggesting that the two nuclei of the spores possessed identical mating type factors.

在交配试验中，源于不同菌株单孢分离物的菌丝原生质体的配对形成具锁状联合的菌落，而源于同一单孢分离物的菌丝原生质体的配对则形成无锁状联合的菌落，暗示担孢子中的两个核具有相同的交配型。

2The protoplast formation and regeneration and fusion conditions of strain ISW330 and AS 1.495 were studied in this thesis. One L-isoleucine high-producing strain SW0370 was screened through protoplast fusion, shake flask screening, genetic marker test and single clone isolation and continuous cultivation for generations.

2确定了亲株ISW330和AS1.495原生质体形成和再生及进行原生质体融合的最佳条件，采用原生质体融合技术最终筛选出一株带有目的遗传标记的L-异亮氨酸高产菌SW0370(Leu~-+Met~-+AEC~r+á-AB~r)。

He terminal cell apoptosis depended on the time of complete degradation of protoplast in cell.During PCD process,all kinds of cell organelles in protoplast promoted secondary wall deposition at different degree.

EM观察结果表明纤维、基本薄壁组织细胞、导管分子的分化过程均是典型的衰退过程，它是由次生壁的沉积和原生质体的衰退这两个密切相关的过程组成。

But GST= 0.156, Nm=1. 588. As a result, the foundation of Youyongchi Avicennia marina population was the result of the migration of hypocotyles and human factors.

这项工作可以为海岸防护林中新引进种类的判定以及为研究种群建立者效应方法的确定提供科学依据。

The two-dimensional CDRC-ADI-FDTD update equations for collision unmagnetized plasma are induced. The unconditional stability of the CDRC-ADI-FDTD formulation for collision unmagnetized plasma is obtained by the examples.

推导了碰撞非磁化等离子体中的二维CDRC-ADI-FDTD迭代公式，并用算例验证了碰撞非磁化等离子体CDRC-ADI-FDTD算法也是无条件稳定的。