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Methods The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase The clpE gene was replaced completely by erm cassette.

用长臂同源多聚酶链式反应方法失活clpE基因，用PCR、测序鉴定缺失菌株，通过动物实验观察clpE基因缺失株毒力改变情况，同时用细胞实验比较clpE基因缺失株和野生菌对宿主细胞的粘附和侵袭能力，最后用实时荧光定量PCR分析自溶素(major autolysin A，lytA)、表面黏附素A(pneumococcal surface adhesion A，psaA)、溶血素(pneumolysin，ply)、肺炎球菌表面蛋白A（pneumococcal surface protein A， pspA和神经氨酸酶（neuraminidase， nanA）的表达。

Objective To investigate the effect of preconditioning high dose vitamin C for the dialyzer on advanced oxidation protein products in hemodialysis patients.

目的 研究大剂量维生素C预冲透析器对血液透析患者晚期氧化蛋白产物(Advanced oxidation protein products，AOPP)的影响。

C reaction albumen (C-reactive Protein, because its can remove precipitation to react with the C polysaccharide of the cellular wall of pneumonic diplococcus and CRP) gets a name [1] , it is the serum β globulin that is 115-140KD relative to molecular quality.

梗概： C反应蛋白(C-reactive protein，CRP)因其能和肺炎双球菌的细胞壁的C多糖起沉淀反应而得名[1]，是相对分子质量为115-140KD的血清β球蛋白。

Objective To observe the effect of Shenghui Keli on AD encephalon neural degeneration induced by A β(β-Amyloid protein) from neural function, biochemical and gene expression. Research the degeneration related to apoptosis of cell and gene expression correlate to apoptosis. Then investigate the mechanisms of Shenghui keli treating AD .

目的 从神经功能形态、生化与基因表达等方面，观察生慧颗粒对β-淀粉样蛋白(β-Amyloid protein，Aβ)诱导脑神经细胞变性的干预作用，以及与细胞凋亡、凋亡相关基因表达的关系，探讨生慧颗粒治疗Alzheimer病的作用机制。

In order to detect the distribution of silica-binding proteins in rice and other graminaceous plants, specific polyclonal antibodies against a silica-binding protein from rice, namely SBP117, are successfully raised by synthesized peptides Which are conjugated with Keyhole Limpet Hemocyanin and used as antigens to immunize rabbits.

为了检测硅结合蛋白在水稻和禾本科植物体内的分布，根据硅结合蛋白(silica-binding protein 117， SBP117)的氨基酸保守片段和天然抗原表位，合成了SBP117的两个肽段做抗原，与匙血蓝蛋白载体偶联后免疫兔子，获得了抗SBP117的专一性抗体。

In this work, hempseed protein isolate were prepared from defatted hempseed and its physicochemical property, functionality, and nutritional property were comprehensively studied.

本课题以脱壳汉麻籽为对象，研究了汉麻籽分离蛋白(hempseed protein isolate，简称HPI)的制备工艺、营养特性和功能特性，通过有限酶法修饰改善其功能特性，并将其应用到焙烤制品中提高面粉的营养价值、改善面团特性和面包的感官品质。

Results One of the cDNA sequences and it's deduced amino acid sequence in deer share high homology with a group of cDNA sequences encoding ribosomal protein L27 in human,rat,Canis and mouse and their corresponding amino acid sequence respectively. It has the completed open reading frame as well.

结果 其中的一个序列在核酸及推导的氨基酸序列水平上与编码人、犬、大鼠、小鼠核蛋白体蛋白L27(ribosomal protein L27)cDNA序列和对应的氨基酸序列高度同源，并具有完整的开放阅读框架。

Soy protein isolate is the most refined form of soy protein, which is widely used in food industry and its functions vary with in different products.

中文摘 要：大豆分离蛋白（soy protein isolate， SPI）是大豆蛋白中最为精制的形式，广泛应用于食品工业，在不同的产品中表现出不同的功能性。

Here, we demonstrated in this work that CsrA performs a negative regulator of the cel expression. cel, encoding lysis protein (LysE7), is located downstream of the colicin E7 structural gene in the ColE7 operon. Lysis protein was essential for colicin release and causes a decline in culture turbidity as well as lethality of the host cell when overproduced. Western blotting analysis of the level of LysE7 in the wild-type and csrA mutant strains was examined.

在本论文中，我们进一步发现到CsrA对於cel 基因的表现具有抑制的现象。cel 基因位在质体ColE7-K317上的E7大肠杆菌操纵子，可以制造出溶菌蛋白质(lysis protein， LysE7)，协助大肠杆菌素及免疫蛋白质的复合体运送至菌体外；但当菌体产生过多的溶菌蛋白质，则会导致宿主细胞的死亡，所以溶菌蛋白质的表现必须受到严密的调控，避免菌体产生过量，而CsrA则是目前第一个被发现到能调控溶菌蛋白质表现的因子。

To explor to change of the protein expression of Mitogen activated protein kinase in rat airway smooth muscle.

目的 探讨缺氧情况下鼠气道平滑肌细胞丝裂原活化蛋白激酶(mitogen activated protein kinase， MAPK)的变化。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。