查询词典 original binding

The central issue of this paper is the study of DNA-binding proteins interacted with DNA replication origin. The paper presented here includes there parts which are related as well as independent of each other:(1). Identification and partially purification of DNA-binding proteins interacted with eukaryotic DNA replication origin;(2) Specific binding of Hela cell proteins to Samian Virus 40 replication origin with the cruciform structure (Stem-Loop structure);(3) The study of DNA-binding protein as a tumor marker.

本文对DNA结合蛋白的研究主要包括三个即有关联，又相对独立的方面：（1）猿猴肾脏细胞复制起始区DNA结合蛋白的鉴定和部分纯化；（2）Hela细胞中DNA结合蛋白对含茎环结构的SV40复制起始区DNA的特异性结合；（3）DNA结合蛋白做为肿瘤征兆物的研究。

Radiolabeled pharmacological binding assay showed: 1 BmK AS bound specifically to a single class of non-interacting binding sites on both rat brain and cockroach nerve cord synaptosomes with high affinity and low low capacity. The association of BmK AS with both synaptosomes was quick to reach equilibrium, but the dissociation seemed to be only partial. The specific binding of BmK AS was independent of membrane potential, and couldn't be modified by veratridine. Moreover, the binding of BmK AS could be inhibited by native BmK AS, BmK AS1, BmK IT2 and BmK IT on both synaptosomes, but not or partially inhibited by BmK Ⅰ and suggesting BmK AS type neurotoxins might bind to a distinct receptor site on mammal and insect sodium channels with a similar manner from β-scorpion toxins.

二。蝎毒素多肽配体与哺乳动物和昆虫靶离子通道受体的药理结合特性及其靶结合位点的研究：经放射性标记结合和竞争抑制实验表明：1）BmK AS在大鼠脑和蟑螂神经索突触体上有单一非协同性高亲和力低结合容量结合位点，能快速结合并达到平衡，但仅能部分解离，其结合不依赖于膜电位，也不受藜芦碱调节，天然的BmK AS，BmK AS-1，BmK IT2和BmK IT可抑制〓AS与两种突触体的特异结合，而BmK Ⅰ无或仅有部分抑制效应。

Of the three binding modes, the intercalative binding is thought to be the strongest binding because it is a type of binding caused by the intercalative molecule plane sandwiched between the aromatic heterocyclic and base group pairs of DNA.

本论文的主要目的是在研究鬼臼金属配合物和铁/锰原子簇化合物与DNA相互作用的基础上，采用紫外光谱、荧光光谱、凝胶电泳和粘度测量等多种研究手段研究了其与DNA的相互作用。

Of the three binding modes, the intercalative binding is thought to be the strongest binding because it is a type of binding caused by the intercalative molecule plane sandwiched between the aromatic heterocyclic and base group pairs of DNA.

本论文的主要目的是在研究鬼臼酰肼金属配合物和铁/锰原子簇化合物与DNA相互作用的基础上，采用紫外光谱、荧光光谱、凝胶电泳和粘度测量等多种研究手段研究了其与DNA的相互作用。

An antibody binding to IGF-IR and inhibiting the binding of IGF-I and IGF-II to IGF-IR which is characterized in that said antibody is a is ofIgG1 isotype, b shows a ratio of IC50 values of inhibition of the binding of IGF-I to IGF-IR to the inhibition of binding of IG-II to IGF-IR of 1:3 to 3:1, c inhibits for at least 80% at a concentration of 5 nM IGF-IR phospohrylation in a cellular phosphorylation assay using 3T3 cells providing 400,000 to 600,000 molecules IGF-IR per cell in a medium containing 0.5% heat inactivated fetal calf serum when compared to such an assay without said antibody, and d shows no IGF-IR stimulating activity measured as IGF-IR phophorylation at a concentration of 10 M in a cellular phosphorylation assay using 3T3 providing 400,000 to 600,000 molecules IGF-IR per cell in a medium containing 0,5% heat inactivated fetal calf serum when compared to such an assay without said antibody has improved properties in antitumor therapy.

一种已经提高了抗肿瘤治疗的特性的抗体，所述抗体结合IGF-IR并且抑制IGF-I和IGF-II与IGF-IR结合，其特征在于所述抗体a是IgG1同种型，b显示其对IGF-I与IGF-IR结合的抑制的IC 50 值与其对IGF-II与IGF-IR结合的抑制的IC 50 值的比率为1∶3-3∶1，c当与没有所述抗体的这种测定比较时，其在5nM的浓度上，在包含0.5％的热灭活胎牛血清的培养基中，使用3T3细胞的细胞磷酸化测定中，抑制至少80％的IGF-IR磷酸化，所述3T3细胞提供400，000-600，000分子IGF-IR/细胞，和d当与没有所述抗体的这种测定相比，在包含0.5％的热灭活胎牛血清的培养基中，使用3T3的细胞磷酸化测定中，其在10μM的浓度上没有显示作为IGF-IR磷酸化所测量的IGF-IR刺激活性，所述3T3提供400，000-600，000分子IGF-IR/细胞。

The experiments confirm that Nuclear fast red bindto DNA to form a 1:1 association complex with the binding constant 7.29×10~5 L/mol,and the major binding mode of Nuclear fast red to DNA is electrostatic binding.However, at high iron strength, the major binding mode changes into intercalativebinding. Part four is about investigation on the interaction of purpurine with DNA.

结果显示，核固红与DNA主要以静电作用模式结合，结合位点为1，结合常数为7.29×10~5L／mol；而在高离子强度下，其主要作用模式改变为嵌插作用；第四部研究了红紫素与DNA的相互作用。

The interaction between MT and AA was expressed by infrared spectra, the functional groups of -SH in MT had lone pair electrons, which could formπ-πwith -C=O in AA. The equilibrium binding experiment and Scatchard analysis showed, at least three kinds of binding sites would exist when MT-imprinted polymer formed, the most adsorption capacities were 3.3896mg/g, 0.9362mg/g, 1.6583mg/g respectively; most terminal thiolate sulfurs in MT were polymerized as binding sites in the process of polymerization, which could observed from TEM, binding capability of other thiolate sulfurs was weak since spatial hindrance existed.

采用红外光谱研究了加入金属硫蛋白后功能单体丙烯酸官能团谱峰的变化，结果表明金属硫蛋白分子中的-SH与丙烯酸中的C=O在氯仿溶液中能形成π-π键，由聚合物的吸附特性和Scatchard分析可以得出，金属硫蛋白印迹聚合物至少存在三种结合位点，其高、中、低的最大吸附量分别为3.3896mg/g、0.9362mg/g、1.6583mg/g；通过透射电镜可以观察到，金属硫蛋白在聚合过程中主要以分子两端的巯基作为结合位点，不同位置的巯基受到的空间位阻不同，导致产生了三种不同的结合位点。

The objective of the last part is to, primarily, check the binding potentiality of the brain tissues with PrPc and then to determine the brain subfractions which have the most abundant binding proteins for the sake of further isolation of binding proteins, and then to identify and characterize these binding candidates.

新发现、新观点、新创造：首先，目前发现的所有朊蛋白的结合分子由于获取方法所限尚未有证据表明其直接结合，而且对结合功能也多为推测，本研究的立意在于从功能着手，分离和鉴定朊蛋白受体，并进一步鉴定其生理功能。

He was only to happy to help out with the display shoes and shipped over a boxed pair of original metallic Jordan I's along with original Fire Red V's, original Carmine VI's, original Powder Blue IX's and original Chicago X's.

他也是唯一乐意帮助这次展览的人，他邮寄过来一箱鞋子，包括一双原版金属色的乔1，原版火红色的乔5，原版深红色的乔6，原文粉蓝色的乔9，以及在芝加哥推出的原版乔10。

They (1) describe the same thitlgs, behaviors or conditions opposite or contrary to the spatial direction as the original characters describe;(2) describe the images reverse to which the original characters describe;(3) give meanings completely opposite to those of original characters;(4) are used to distinguish similar but different things with the original characters;(5) give new meanings which are spread out from those of original characters;(6) expand more abstract meanings.

其意义大致有以下几种类型：（1描绘与原字所指事物、行为或状态在空间方向上呈相反或相背关系的同类事物、行为或状态；(2)与原字分别描绘同一事物的正反两面形象；(3)新字义是对原字义的完全否定；(4)新字与原字用以区别形象相似的两种不同事物；新字的意义由原字意义顺衍而来；(6)引申出较为抽象的含义。

Chinese traditional virtue is humility and wariness,the compliment and praise to the others should be declination

中国人的传统美德是谦虚谨慎，对别人的恭维和夸奖应是推辞。如

We bought this house on the never-never.

我们以分期付款的方式买下这座房子。