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Chapter 5: The fluorescence quenching reactions of barbaloin with serum albumin in solution were studied. The quenching mechanism of serum albumin by barbaloin was interpreted using the Stern-Volmer mechanism. The binding constant K values and the number of binding sites were calculated, respectively.

第五章：首次研究了芦荟甙与血清蛋白在溶液中的相互作用，利用Stern-Volmer方程解释了芦荟甙对血清蛋白的荧光淬灭，并测定了不同温度下的结合常数及结合位点数。

Absorption spectral studies show that binding to the serum albumins leads to a bathochromic shift of the monomer band together with a notable intensity change. Furthermore, the number of binding sites was identified by the absorption spectra.

吸收光谱数据表明，与血清蛋白结合后，噻菁染料单体的吸收峰发生红移，同时强度也有很大变化；还通过吸收光谱计算确定了噻菁染料与血清蛋白的结合位点数。

Seven colorimetric methods Biuret, Lowry, a modified Lowry technique using bicinchoninic acid, Coomassie brilliant blue G (CBBG dye-binding in phosphoric acid, perchloric acid or hydrochloric acid, and bromophenol blue dye-binding were evaluated for determination of protein concentration in human whole saliva.

本实验以七种定量蛋白质之方法（Biuret法，Lowry法，BCA法，CBBG染料结合法、BPB染料结合法），利用三种参考蛋白质评估测定人类唾液蛋白质浓度之差异性。

Using HPLC separation and receptor binding assay, the present study demonstrated that the principle in the water extract of Huangqin acted on the BDZ binding site was not baicalin, wogonin or chrysin. In vivo study, WEH had no significant effect on pentylenetetrazol-induced clonic seizures in mice, although it could inhibitmaximal electroshock-induced tonic seizures with an ED50 of 3.6g/Kg.

本研究中，藉由高效率液相层析术与接受器结合实验评估，证实黄苓水抽提物中作用在BDZ结合处之主要成分，并不是黄苓甘、泛黄苓素或chtysin。

All experimental results show that the mode of binding of Sm3 to DNA is mainly electrostatical binding.

实验综合表明，Sm3与DNA之间的结合主要为外部静电作用方式。

Objective To express and locate a fusion gene consisting of c-Myb DNA-binding domain and Myeloid elflike factor AML1-binding region by using baculovirus vector.

目的 将C-Myb的DNA结合域与MEF的AML1结合区组合成的MM融合基因在杆状病毒中进行表达和细胞定位。

Results: Mel specific binding sites were det ermined in 16 kinds of human embryonic tissue and this binding could be inhibit ed by GTPγS, supporting the theory that MR is coupled to inhibitory G-proteins system.

结果：经饱和分析、动力学分析及特异性分析证明人胚胎心、肝、肾及甲状腺等16种组织均存在Mel特异结合位点，亚细胞分布研究表明这些组织中以细胞核及线粒体MR含量最高；GTPγS能够抑制Mel与其受体的结合，使MR的结合容量或亲和力降低。

Esculetin had the highest affinity toward the binding site of XO, and this was due to hydrogen bonding interactions. The hydroxyl group at C-6 formed hydrogen bonds with the carboxyl group Glu802 that played critical role on XO binding.

在8种香豆素衍生物中发现esculetin与黄嘌呤氧化酶的亲和力最高，因为在esculetin的结构上，C-6的OH基团可与麸胺酸802的COOH基团形成氢键，此键结对於抑制黄嘌伶氧化酶活性是很重要的，因而增加了两者结合的稳定性。

As well using flameless atomic absorption spectophotometer toanalyse the amount of platinum binding to DNA,we can fand that after 1hour at 37℃ the amount of platinum binding to DNA for BGC-823 acted byCDDP 20ug/ml in hypotonic solution was 30.1±6.2ng/mg·DNA,whilethe isotonic groups were 6.4±1.5ng/mg·DNA.

采用非火焰原子吸收光谱法检测DNA结合铂含量发现，BGC-823经20μg／ml CDDP，37℃作用1小时，低渗给药组DNA结合铂含量为30.1±6.2ng／mg·DNA，等渗给药组DNA结合铂含量为6.4±1.5ng／mg·DNA，两者差异显著(P＜0.05)，该结果表明低渗溶液能显著增强CDDP与其作用靶点—DNA的交联。

However, the binding patterns between the HU-induced and uninduced HEL cells were different. The data in Southwestern-blot analysis showed that the molecular weights of the GATA factors binding to the HS2core DNA sequence were 43kd and 47kd respectively.

反义寡核苷酸技术及RT-PCR的结果表明，GATA-1的反义核苷酸抑制了羟基脲诱导的β-珠蛋白基因的表达，进一步证明了GATA-1因子在羟基脲诱导的HEL细胞分化过程中是不可缺少的促进因素。

We stopped by the old fashion ice cream parlor Manhattan Beach Creamery for some refreshment. The "No Shoes, No Shirt, No Problem" sign on the wall epitomizes the beach lifestyle.

我们去商店区的一家老式冰淇淋店坐坐，墙上挂著「没鞋，没上衣，没问题」的牌子完全捕捉了海滩生活的随性及风格。

"If therefore", argues Bellarmine we can offer our prayers and our satisfactions in behalf of those detained in purgatory, because we are members of the great body of Christ, why may not the Vicar of Christ apply to the same souls the superabundant satisfaction of Christ and his saints--of which he is the dispenser?

" "因此，如果"，认为贝拉明（德indulgentiis ，十四）"我们可以提供我们的祈祷和我们满意的代表那些被拘留在炼狱，因为我们是成员的伟大基督的奥体，为什麼可能不是牧师基督适用於同样的灵魂满意的过剩是耶稣和他的圣人-他是在配药？