查询词典 original binding

Radio-ligand binding assay was applied to determine the maximum binding and affinity changes of nicotinic acetylcholine receptor on five weeks old male Sprague-Dawley rat's lateral pterygoid muscle and superficial masseter muscle after the function mandibular advancement.

本研究在以往研究的基础上，首次对功能矫形的神经肌肉调控机理进行探索。应用放射受体分析法，从膜受体蛋白分子水平定量研究功能矫形前伸青春期SD大鼠（5周龄）下颌后，下颌前伸肌细胞膜烟碱样乙酰胆碱受体最大结合容量和亲和性的变化。

According to the thermodynamic parameters, the main binding forces between them are hydrogen bonding and Van der Waals interactions. Furthermore, the binding distance and transfer efficiency of the interaction energy between BSA and the pyrano [4,3-b] pyran derivative were obtained according to the theory of Forster non-radiation energy transfer.

根据Forster非辐射能量转移机理，测定了这种吡喃并[4，3-b]吡喃衍生物与牛血清白蛋白相互结合时，能量给体-受体间的作用距离和能量转移效率，并用同步和三维荧光技术讨论了其衍生物对牛血清白蛋白构象的影响。

Combination of pyrrole binding site and amide NH generated a new kind anions binding site, pyrrolic amide.

在尘命体内，蛋白质对阴离子的识别主要是通过肽链中酰胺NH与阴离子间的氢键来实现的。

Results Several amino acid substitutions located in antigenic sites or receptor binding sites were found more in the isolates than the current vaccine virus or the isolates from previous year. Amino acid substitution was found in 3, 140, 142, 144, 145, 158, 159, 189, 192, 193, 198, 204, 225, 226 and 227 positions in the isolates during 2003-2008, more amino acid substitutions took place in antigenic determinant A, B and receptor binding site.

结果 每个流行期分离的毒株都较当年的疫苗株或前一年的毒株出现了位于不同抗原决定簇或者受体结合位点的氨基酸替换。2003-2008年不同流行期分离株在3，140、142、144、145、158、159、189、192、193、198、204，225、226和227位点氨基酸发生了变化，多数的改变发生在抗原决定簇A、B及RBS。

SDS-PAGE and Western blot of culture supernatant showed that hsIL-1RⅠ was secretively expressed. Recptor-ligand binding assays showed that the recombinant hsIL-1RⅠ has receptorligand binding activity.

发酵液经SDS-PAGE和Western blot证实分泌表达了sIL-1RI，经蛋白印迹受体配基结合反应证实分泌表达的sIL-1RI具有配基受体结合的生物学活性。

Upon initiation of PCR after the denaturation phase, the single-stranded DNA fragments flanked by inverted terminal repeats may form either self-annealing "pan-like" structures (preventing the primer binding to its complementary binding sites and suppressing the PCR) or DNA/primer hybrid structures.

经聚合酶链反应开始后，变性阶段，单链 DNA片段两侧末端反向重复序列可能两种自我退火"泛，如"结构（防止引物结合到其互补性结合位点和制止的PCR ）或DNA /引物混合结构。

The calculated amino acids sequence matches the known 30aa peptide sequence which shows high homology with some monocotylous mannose-binding lectin from Epipactis helloborine, Listera ovata and snowdrop . Such results shows that GAFP maybe a kind of new mannose-binding lectin of Gastrodia elata Bl.

根据GAFP与火烧兰、二月兰和雪花莲中的单子叶甘露糖结合凝集素的序列高度相似这一现象，我们可以推测GAFP可能是天麻中的一种甘露糖结合凝集素。

The regulation of gene expression is to have relations to the assembly of the DNA binding proteins which bind to DNA to initiate the transcription. Some of these proteins have the ability to promote bending or looping of DNA. The FIS protein regulates gene expression in Escherichia coli by this property of bending DNA. There are many researches to discover the mechanism of FIS protein binds to DNA. However the experiment data only show the supposititious results and figure out the model of FIS-DNA complex structure. This research uses molecular dynamics simulation to compute the detail of the interaction between FIS and DNA. We observed FIS prominently affects the DNA bending by the type of local bending. Analyzing the DNA structure properties, roll and slide, also demonstrates the local bending type and region. The hydrogen bonds between FIS and DNA are analyzed to compare with the experiment data. Finally we compute the binding free energy between FIS and DNA to estimate the result of simulation and compare with the value of λ-repressor-DNA complex.

基因的表现与结合於DNA促使DNA转录作用起始的DNA结合蛋白组装有关，一些这类的蛋白质具有促使DNA扭曲或将DNA形成环状的能力，FIS蛋白质即是藉由扭曲DNA的特性调控大肠杆菌基因的表现，目前已有很多的研究发现FIS与DNA结合的机转，然而这些实验的数据仅能显示推测性的结果，进而利用这些结果推论出FIS与DNA聚合体结构的模型，本研究利用分子动态模拟的方式计算FIS与DNA交互作用的细节，我们观察到FIS主要影响DNA的扭曲方式为DNA区域扭曲的类型，并且分析DNA结构特性roll与slide也指出扭曲方式为区域扭曲与其扭曲的区域，我们也分析FIS与DNA之间的氢键，并且与实验的资料作比较，最后我们计算FIS与DNA之间的结合自由能评估模拟的结果，并且计算出的值和λ-repressor与DNA聚合体结构的实验值作比较。

Ca2+ and EGTA-containing gel electrophoresis, combined with the corresponding terbium fluorescence on the basis of selective occupation of calcium binding sites, were applied to identify the potential calcium binding domains in PS II particles.

采用含Ca2+和含EGTA的凝胶电泳，结合竞争性取代钙结合位点的钙离子的铽荧光的方法检测光系统II中的钙结合蛋白。

According to the fluorescence measurement results from mixed solution, the association constant estimated for thioridazine hydrochloride-BSA binding and the number of the binding site with Scatchard analysis were 1.2×104 L/mol and 0.98, respectively.

将盐酸硫利达嗪与牛血清白蛋白以不同比例混合并在37 ℃下培养，以流动注射透析采样技术从混合液中分离出游离盐酸硫利达嗪，在线氧化为强荧光化合物后测定其浓度；应用Scrathard方程分析所测数据，求得盐酸硫利达嗪和牛血清白蛋白的结合常数为1.2×104 L/mol，结合位点数为0.98；根据热力学参数推断，盐酸硫利达嗪与牛血清白蛋白之间以疏水作用力为主；进一步基于荧光猝灭现象和Fster非辐射能量转移理论，得到盐酸硫利达嗪小分子与牛血清白蛋白之间的能量转移效率( E =0.497)和结合距离( r 0=3.27 nm)。

There had been a moment during the breakfast meeting, though, after the backslapping and the small talk and when all of us were seated, with Vice President Cheney eating his eggs Benedict impassively and Karl Rove at the far end of the table discreetly checking his BlackBerry, that I witnessed a different side of the man.

那次早餐会期间，在表示过关心和寒暄以后，我们所有人坐了下来，我注意观察了一下，副总统切尼面无表情地吃着班尼迪克蛋（Eggs benedict，源自美国，以英式松饼、火腿、水煮蛋以及荷兰酱组合而成），卡尔。罗夫在餐桌的远端谨慎地经常查看他的黑莓手机，我目睹了他所不为人知的一面。

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